Smooth muscle cells from rat heart secreted extracellular matrix components at high rates for many generations in culture. The proteins remained anchored to the culture dish and were characterized after removal of cellular material with sodium dodecyl sulfate. Sequential enzyme digestion demonstrated presence least three components, including glycoprotein(s), elastin, collagen. Prolonged extraction detergent under reducing conditions solubilized a fucosylated glycoprotein having an apparent...

The biosynthesis of the peptide antibiotic gramicidin S involves successive peptidyl transfer reactions between intermediates bound in thioester linkages to two active enzyme fractions, I and II. Fraction II activates recemizes phenylalanine, then initiates by catalyzing a reaction carboxyl group D-phenylalanine, an enzymic sulfhydryl group, free imino L-proline, one four L-amino acids all linked their functions separate groups on fraction I. Successive this type centers multienzyme complex...

1. The effects of added AMP on carbohydrate metabolism were investigated in pigeon-liver homogenates, which can degrade glucose and synthesize it from lactate. Suitable experimental conditions established for studying such effects, including the addition P(i) (20mm) to stabilize adenine nucleotides supplementation with NAD(+) (0.5mm). 2. Lactate increased rate oxygen consumption kept concentration ATP high that relatively low. 3. Added (1.25-5mm) raised net removal inhibited formation...

The retinyl palmitate fat tolerance test was used to measure chylomicron remnant clearance in 10 normal subjects (apolipoprotein E [apo E] isotypes 3 or 4 only), 6 normolipidemic apo E2/2 homozygotes and 5 familial hypercholesterolemic homozygotes. Skin fibroblasts with fully upregulated LDL receptors from the latter degraded rabbit 125I-beta VLDL vitro at rates ranging less than 10-48% of normal. Experiments vivo revealed no significant differences between homozygous (FHH) assessed on basis...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTyrocidine biosynthesis by three complementary fractions from Bacillus brevis (ATCC 8185)Fritz Lipmann, Robert Roskoski, Wieland Gevers, and Horst KleinkaufCite this: Biochemistry 1970, 9, 25, 4839–4845Publication Date (Print):December 8, 1970Publication History Published online1 May 2002Published inissue 8 December 1970https://doi.org/10.1021/bi00827a002RIGHTS & PERMISSIONSArticle Views175Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views...

1. The intracellular location and maximal activities of enzymes involved in phosphoenolpyruvate synthesis have been investigated pigeon liver. Enolase pyruvate kinase were cytoplasmic, the 50-60 180-210mumoles/min./g. dry wt. at 25 degrees respectively. Phosphoenolpyruvate carboxykinase was present exclusively, nucleoside diphosphokinase predominantly, mitochondria; particles had to be disrupted elicit activities, which 27-33 400-600mumoles/min./g. all four did not change significantly...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation of enzyme-bound peptide intermediates in tyrocidine biosynthesisFritz Lipmann, Robert Roskoski, Jr., Horst Kleinkauf, and Wieland GeversCite this: Biochemistry 1970, 9, 25, 4846–4851Publication Date (Print):December 8, 1970Publication History Published online1 May 2002Published inissue 8 December 1970https://pubs.acs.org/doi/10.1021/bi00827a003https://doi.org/10.1021/bi00827a003research-articleACS PublicationsRequest reuse...

The nucleic acid-independent biosynthesis of the peptide antibiotic gramicidin S results from interaction an enzyme bearing phenylalanine in activated form with a polyenzyme system charged other four component amino acids. After reaction ATP, magnesium, and any or all its acid substrates, (mol wt 280,000) yields complexes containing AMP respective acids proportion 1 to 2. Similar are formed by another 100,000) on incubation L- D-phenylalanine. probably bound as aminoacyl adenylates then...

Sodium butyrate is very often used to alter gene expression in cultured cells. In this study, we examined the effects of compound on various cellular events WI-38 human embryonic lung fibroblasts culture. During a 16-20-h treatment at sodium concentrations between 5 and 20 mM, no adverse cell morphology were observed. However, division DNA synthesis reversibly inhibited, latter by 85, 80, 70% 5, 10, respectively. Although overall protein synthetic activity was not significantly affected, RNA...

Peptide antibiotics are produced by a variety of microorganisms in special physiological circumstances (see Gottlieb and Shaw, 1967). Certain structural features these substances indicate that the biosynthetic mechanisms must differ from those proteins. For example, residues other than 20 or so L-α-amino acids found proteins frequently present (including D-amino acids, hydroxy fatty acids), molecules often cyclic, analog-type substitutions individual very common. Much evidence (well...

The binding and catabolism of low density lipoprotein (LDL) were studied in cultured skin fibroblasts and/or Epstein-Barr virus-transformed lymphoblastoid cells from 20 familial hypercholesterolemic (FH) homozygotes 14 Afrikaner kindreds. Cells available heterozygotes also analyzed. Good resolution between the normal, heterozygote, homozygote groups was obtained on basis these assays using either cell type. Results with allowed classification 13 kindreds as being typically...

Journal Article Loss of type I procollagen gene expression in SV40-transformed human fibroblasts is accompanied by hypermethylation these genes Get access M.Iqbal Parker, Parker * Department Medical Biochemistry, University Cape Town SchoolObservatory 7925, Republic South Africa To whom correspondence should be addressed. Search for other works this author on: Oxford Academic PubMed Google Scholar Karen Judge, Judge Wieland Gevers Nucleic Acids Research, Volume 10, Issue 19, 11 October 1982,...