A5064138399- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Cancer-related Molecular Pathways
- Melanoma and MAPK Pathways
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Protein Degradation and Inhibitors
- RNA Research and Splicing
- interferon and immune responses
Salk Institute for Biological Studies
2017-2018
Institute of Molecular Biology
2014
The role of individual subunits in the targeting and function mammalian BRG1-associated factors (BAF) complex embryonic stem cell (ESC) pluripotency maintenance has not yet been elucidated. Here we find that Bromodomain containing protein 9 (BRD9) Glioma tumor suppressor candidate region gene 1 (GLTSCR1) or its paralog GLTSCR1-like (GLTSCR1L) define a smaller, non-canonical BAF (GBAF complex) mouse ESCs is distinct from canonical ESC (esBAF). GBAF esBAF complexes are targeted to different...
ARID1A, a subunit of the SWI/SNF chromatin remodeling complex, is frequently mutated in cancer. Deficiency its homolog ARID1B synthetically lethal with ARID1A mutation. However, functional relationship between these homologs has not been explored. Here, we use ATAC-seq, genome-wide histone modification mapping, and expression analysis to examine colorectal cancer cells lacking one or both ARID proteins. We find that dominant role maintaining accessibility at enhancers, while contribution...
Cyclin-dependent kinase 7 (CDK7) activates cell cycle CDKs and is a member of the general transcription factor TFIIH. Although there substantial evidence for an active role CDK7 in mRNA synthesis associated processes, degree its influence on global gene-specific mammalian species unclear. In current study, we utilize two novel inhibitors with high specificity to demonstrate restricted but robust impact gene vivo vitro-reconstituted reactions. We distinguish between relative low- high-dose...