A5091687285- Hormonal and reproductive studies
- Muscle metabolism and nutrition
- Pharmacology and Obesity Treatment
- Analytical Chemistry and Chromatography
- Pharmacogenetics and Drug Metabolism
- Pharmacological Effects and Assays
- Nuclear Issues and Defense
- Hormonal Regulation and Hypertension
- Crystallization and Solubility Studies
- Twentieth Century Scientific Developments
- Veterinary Equine Medical Research
- Mass Spectrometry Techniques and Applications
- Veterinary Pharmacology and Anesthesia
- Metabolomics and Mass Spectrometry Studies
- Analytical Methods in Pharmaceuticals
- Radiation Effects and Dosimetry
Australian National University
2014-2021
The untargeted detection of phase II metabolites is a key issue for the study drug metabolism in biological systems. Sensitive and selective mass spectrometric (MS) techniques coupled to ultrahigh performance liquid chromatographic (UHPLC) systems are most effective this purpose. In study, we evaluate different MS approaches with triple quadrupole instrument bis-sulfate metabolites. Bis-sulfates 23 steroid were synthesized their behavior was comprehensively studied. ionized preferentially as...
The hydrolysis of sulfate ester conjugates is frequently required prior to analysis for a range analytical techniques including gas chromatography‐mass spectrometry (GC‐MS). Sulfate may be achieved with commercial crude arylsulfatase enzyme preparations such as that derived from Helix pomatia but these contain additional activities glucuronidase, oxidase, and reductase make them unsuitable many applications. Strong acid can also used hydrolyze esters this lead analyte degradation or...
In 2012, seized capsules containing white powder were analyzed to show the presence of unknown steroid-related compounds. Subsequent gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) investigations identified a mixture 3α- 3β- isomers novel compound; 3-chloro-17α-methyl-5α-androstan-17β-ol. Synthesis authentic reference materials followed by comparison NMR, GC-MS chromatography-tandem mass (GC-MS/MS) data confirmed finding new 'designer' steroid. Furthermore,...
In vitro technologies provide the capacity to study drug metabolism where in vivo studies are precluded due ethical or financial constraints. The metabolites generated by can assist anti‐doping laboratories develop protocols for detection of novel substances that would otherwise evade routine screening efforts. addition, professional bodies such as Association Official Racing Chemists (AORC) currently permit use in‐vitro‐derived reference materials confirmation purposes providing additional...
Abstract Calcium dobesilate (CD) is a synthetic venoactive drug used in veterinary medicine to treat equine navicular disease. Etamsylate haemostatic agent horses for the treatment of exercise‐induced pulmonary haemorrhage. Both etamsylate and CD dissociate circulatory system with 2,5‐HBSA as active drug. The aim research was be able provide detection time (DT) advice from pharmacokinetic (PK) studies Thoroughbred better inform trainers, their surgeons, prescribing these substances...
Abstract Hemapolin (2α,3α‐epithio‐17α‐methyl‐5α‐androstan‐17β‐ol) is a designer steroid that an ingredient in several “dietary” and “nutritional” supplements available online. As unusual chemical modification to the A‐ring could allow this compound pass through antidoping screens undetected, metabolism of hemapolin was investigated by vivo equine drug administration study coupled with GC‐MS analysis. Following synthetically prepared thoroughbred horse, madol...
Samples of the 'dietary supplement' Furazadrol sourced through internet have been reported to contain designer anabolic androgenic steroids [1',2']isoxazolo[4',5':2,3]-5α-androstan-17β-ol (furazadrol F) and [1',2']isoxazolo[4',3':2,3]-5α-androstan-17β-ol (isofurazadrol IF). These an isoxazole fused A-ring were designed offer activity while evading detection, raising concerns over potential for abuse this preparation in sports. The metabolism (F:IF, 10:1) was studied by vivo methods...