A5061595252- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Hematopoietic Stem Cell Transplantation
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Antenna Design and Analysis
- Immunodeficiency and Autoimmune Disorders
- Acute Lymphoblastic Leukemia research
- Full-Duplex Wireless Communications
- Multiple Myeloma Research and Treatments
- Parvovirus B19 Infection Studies
- Polyomavirus and related diseases
- Glycosylation and Glycoproteins Research
- Lymphoma Diagnosis and Treatment
- T-cell and Retrovirus Studies
- CRISPR and Genetic Engineering
Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes Angers
2010-2022
Université d'Angers
2017-2022
Nantes Université
2008-2022
Inserm
2006-2018
Centre National de la Recherche Scientifique
2017-2018
Centre Hospitalier Universitaire de Nantes
2005-2006
Université de Tours
2006
Centre Hospitalier Universitaire de Tours
2006
Abstract Recent studies have demonstrated that a large fraction of human gamma delta PBL recognize Ag prokaryotic and eukaryotic origins, respectively found in hydrosoluble mycobacterial extracts on the Daudi Burkitt's lymphoma cells. The structural basis recognition these been presently studied detail, through analysis panel thymus- peripheral blood-derived T-cell clones. Our results suggest mycobacteria-reactive subsets are strictly overlapping hence responses against two closely related....
In the context of transplantation, donor and virus-specific T-lymphocyte infusions have demonstrated dramatic potential T cells as immune effectors. Unfortunately, most attempts to exploit T-cell system against nonviral malignancies in syngeneic setting been disappointing. contrast, treatments based on monoclonal antibodies (Abs) clinically successful clinical relevance several antigens therapeutic targets importance antibody-dependent cellular cytotoxicity (ADCC) pathway. present study, we...
We report herein the results we obtained and limitations experienced during production use of a bank Epstein-Barr virus (EBV)-transformed human cytotoxic T lymphocytes (EBV-CTLs). To assess feasibility toxicity this strategy, selected stored, in liquid nitrogen, 4 billion EBV-CTLs from each 13 donors. Subsequently, multicenter phase I/II study, 11 patients with EBV-associated lymphoma resistant to conventional treatments received 1–3 doses 5 million EBV-CTLs/kg 0–4 compatibilities for...
Human memory T cells are comprised of distinct populations with different homing potential and effector functions: central that mount recall responses to Ags in secondary lymphoid organs, confer immediate protection peripheral tissues. In the present study we demonstrate a proportion express FcgammaRIIIa (CD16), perforin positive, directly mediate Ab-dependent cytotoxicity ex vivo. This particular alphabeta lymphocyte subset has morphology large granular lymphocytes, increases...
To take advantage of the large number well-characterized mouse immunoglobulins (IgGs) for study antibody-dependent cell-mediated cytotoxicity (ADCC) in human cells, we armed cytotoxic lymphocytes with a receptor Fc portion IgG antibodies. The ΝΚ-92 natural killer cell line was transduced gene (mCD16), which stably expressed on surface (referred to as NK-92 (mCD16) ). When tested against B-lymphoblastoid (BLCL) coated anti-CD20 IgG1, IgG2a or IgG2b monoclonal antibodies (mAbs), newly enabled...
Analysis of a large number unrelated bone marrow transplantations (BMT) has shown that HLA-DP incompatibility did not detectably influence the risk for acute graft-versus-host disease (aGVHD). Accordingly, it was proposed determinants function as transplantation antigens in same way HLA-A, -B, or -DR. We have previously (as well -DQ, -DR)-specific T cells could be isolated from skin biopsies patients who developed an aGVHD after semiallogeneic BMT. Nevertheless, whether single mismatched...
The present work was designed to compare two mechanisms of cellular recognition based on Ab specificity: firstly, when the anti-HER2 mAb trastuzumab bridges target cells and cytotoxic lymphocytes armed with a Fc receptor (ADCC) and, secondly, HER2 positive are directly recognized by chimeric antigen (CAR). To these mechanisms, we used same effector (NK-92) signaling domain (Fc ε RI γ ). NK-92 cell line transfected either RIIIa-Fc (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
We exploited traceable gene tagging in primary human T cells to establish the composition and dynamics of seven canonical TCR-induced protein signaling complexes (signalosomes) using affinity purification coupled with mass spectrometry (AP-MS). It unveiled how LAT adaptor assembles higher-order molecular condensates revealed that proximal TCR-signaling network has a high degree qualitative quantitative conservation between CD4+ CD8+ cells. Such systems-level also extended across mouse...
Abstract The two main constraints that currently limit a broader usage of T cell therapy against viruses are the delay required to obtain specific cells and safety selection procedure. In present work we developed generally applicable strategy eliminates need for APC timing reasons, infectious viral strains concerns. As model, used lymphocytes immunodominant CMV phosphoprotein pp65. PBMC from healthy seropositive donors were first depleted IL-2R α-chain CD25+ then stimulated 24–96 h with...
Twenty percent of breast cancer adenocarcinomas overexpress the oncogene c-erb-2 that is recognized by humanized anti-Her2/neu monoclonal antibody Herceptin. Results from clinical studies suggest antibody-dependent cellular cytotoxicity (ADCC) involved in response Herceptin-treated patients. The purpose current study was to evaluate possibility amplifying vitro CD3−/CD16+ natural killer (NK) cell subset mediates ADCC patients after chemotherapy. Peripheral blood mononuclear cells six taken 2...
Abstract Polyclonal T cell lines specific for EBV proteins have proved efficient in preventing EBV-related immunoblastic lymphoma after allogeneic bone marrow transplantation. To gain insight into the composition of EBV-specific repertoire that ensured patient protection, we performed first time an extensive characterization eight cytotoxic selected vitro against EBV-transformed autologous lymphoblastoid (BLCL). These consist 50–100 distinct clones, which 32–96% are BLCL. Moreover,...
During serial assays designed to amplify natural killer (NK) cells in vitro, we observed that when peripheral blood mononuclear (PBMCs) from human immunodeficiency virus positive (HIV+) patients were stimulated for 2 weeks with an Epstein-Barr virus-infected B lymphoblastic cell line and interleukin-2, a well known procedure NK 44.4±18% CD3+CD16pos T lymphocytes recovered together cells, of which 78.2% expressed αβ T-cell receptor (TCR). To identify the compartment they originated (naive,...
Summary Epstein-Barr Virus (EBV)-transformed B lymphoblastoid cell lines (BLCL) are currently used for numerous applications in cellular immunology. Where protocols destined clinical application concerned, the final choice of assay is made according to a risk/benefit ratio analysis. In this balance use xenogenic or allogenic serum has always been major concern, as it carries both an infectious and immunological risk. So far, unknown whether can be omitted from entire BLCL selection...
To combine the immune potential of T cells and Ab therapy, we others have previously shown that transduction with a fusion receptor binds Fc portion human Ig enable them to mediate Ab-dependent cellular cytotoxicity (ADCC). The receptors described included FcγRIIIa (CD16) coupled different chains intended translate signal. In this work, questioned whether transfection CD16 alone into lymphocytes NK could be sufficient for expression function, or cotransfection transducing chain was...