A5035470258- Pluripotent Stem Cells Research
- Renal and related cancers
- Organ Transplantation Techniques and Outcomes
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Liver physiology and pathology
- Mesenchymal stem cell research
- Renal Transplantation Outcomes and Treatments
- Organ Donation and Transplantation
- Biomedical Ethics and Regulation
- Neuroscience and Neural Engineering
- Liver Disease and Transplantation
- 3D Printing in Biomedical Research
- Congenital Anomalies and Fetal Surgery
- Congenital gastrointestinal and neural anomalies
- Radiation Dose and Imaging
- MicroRNA in disease regulation
- Cardiac Ischemia and Reperfusion
- Angiogenesis and VEGF in Cancer
- Alcohol Consumption and Health Effects
- CAR-T cell therapy research
- Electron Spin Resonance Studies
- Renal cell carcinoma treatment
- Cardiac electrophysiology and arrhythmias
Inserm
2015-2025
Université de Poitiers
2018-2025
Ischémie Reperfusion, Métabolisme et Inflammation Stérile en Transplantation
2023-2024
Hôpital Paul-Brousse
2013-2022
Fédération Hospitalo-Universitaire, Paris Center for Microbiome Medicine
2019-2022
Université Paris-Saclay
2019-2022
Universidade de São Paulo
2017-2021
Centre Hospitalier Universitaire de Poitiers
2018-2020
Université Paris-Sud
2013-2016
Bicêtre Hospital
2014
Cholangiocytes are biliary epithelial cells, which, like hepatocytes, originate from hepatoblasts during embryonic development. In this study we investigated the potential of human stem cells (hESCs) to differentiate into cholangiocytes and report a new approach, which drives differentiation hESCs toward cholangiocytic lineage using feeder-free defined culture conditions. After hepatic progenitors, were differentiated further growth hormone, epidermal factor, interleukin-6, then sodium...
Abstract The use of synthetic messenger RNAs to generate human induced pluripotent stem cells (iPSCs) is particularly appealing for potential regenerative medicine applications, because it overcomes the common drawbacks DNA-based or virus-based reprogramming strategies, including transgene integration in particular. We compared genomic integrity mRNA-derived iPSCs with that retrovirus-derived generated strictly comparable conditions, by single-nucleotide polymorphism (SNP) and copy number...
Rho family GTPases are molecular switches best known for their pivotal role in dynamic regulation of the actin cytoskeleton, but also cellular morphology, motility, adhesion and proliferation. The prototypic members this (RhoA, Rac1 Cdc42) contribute to normal kidney function play important roles structure various cells including tubular epithelial cells, mesangial podocytes. kidney's vital filtration depends on structural integrity glomerulus, proximal portion nephron. Within...
Abstract Human induced pluripotent stem (hiPS) cell technology has already revolutionized some aspects of fundamental and applied research such as study disease mechanisms pharmacology screening. The first clinical trial using hiPS cell‐derived cells began in Japan, only 10 years after the publication proof‐of concept article. In this exciting context, strategies to generate have evolved quickly, tending towards non‐invasive protocols sample somatic combined with “safer” reprogramming...
Abstract Due to organ shortage, clinicians are prone consider alternative type of donors among them deceased after circulatory death (DCD). However, especially using these organs which more graft dysfunction, there is a need better understand mechanistic events ocuring during ischemia phase and leading ischemia/reperfusion injuries (IRI). The aim this study provide dynamic transcriptomic analysis preclinical porcine model kidneys subjected ischemic stress mimicking DCD donor. We compared...
Using drugs to treat COVID-19 symptoms may induce adverse effects and modify patient outcomes. These events be further aggravated in obese patients, who often present different illnesses such as metabolic-associated fatty liver disease. In Rennes University Hospital, several drug hydroxychloroquine (HCQ) have been used the clinical trial HARMONICOV including patients. The aim of this study is determine whether HCQ metabolism hepatotoxicity are worsened patients using an vivo/in vitro...
Ischemia-reperfusion (IR) injury is unavoidable during organ transplantation and impacts graft quality. New paradigms are emerging including preservation at higher temperature than “hypothermia” or “cold”: although 4°C remains largely used for kidney preservation, recent studies challenged this choice. We others hypothesized that a temperature, closer to physiological regimen, could improve For purpose, we an in vitro model of endothelial cells exposed hypoxia-reoxygenation sequence...
Renal transplantation is increasingly associated with the presence of comorbidity factors such as dyslipidemia which could influence graft outcome. We hypothesized that hypercholesterolemia affect vascular repair processes and promote post-transplant renal remodeling through over-expression anti-angiogenic thrombospondin-1 interacting endothelial growth factor-A levels. tested this hypothesis in vitro, vivo a human cohort using (1) cells; (2) kidney auto-transplanted pig subjected (n = 5) or...
Abstract Background and Aims Pluripotent stem cell–derived hepatocytes differentiated in monolayer culture are known to have more fetal than adult hepatocyte characteristics. If numerous studies tend show that this immature phenotype might not necessarily be an obstacle their use transplantation, other applications such as drug screening, toxicological studies, or bioartificial livers reliant on functionality require full differentiation of hepatocytes. New technologies been used improve the...
Abstract Among strategies to limit ischemia/reperfusion (IR) injuries in transplantation, cell therapy using stem cells condition/repair transplanted organs appears promising. We hypothesized that a based on extracellular vesicles (EVs) derived from urine progenitor (UPCs) during hypothermic and normothermic machine perfusion can prevent IR‐related kidney damage. isolated characterized porcine UPCs their (EVs). Then these were used an ex vivo preservation model. Kidneys subjected warm...
Human induced pluripotent stem cells (hiPSCs) hold great promise for cell therapy through their use as vital tools regenerative and personalized medicine. However, the genomic integrity of hiPSCs still raises some concern is one barriers limiting in clinical applications. Numerous articles have reported occurrence aneuploidies, copy number variations, or single point mutations hiPSCs, nonintegrative reprogramming strategies been developed to minimize impact process on hiPSC genome. Here, we...