A5001284635- Pluripotent Stem Cells Research
- Renal and related cancers
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Cardiovascular Function and Risk Factors
- MicroRNA in disease regulation
- Fuel Cells and Related Materials
Universidade Nova de Lisboa
2017-2023
Universidade de São Paulo
2017
Abstract Human induced pluripotent stem (hiPS) cell technology has already revolutionized some aspects of fundamental and applied research such as study disease mechanisms pharmacology screening. The first clinical trial using hiPS cell‐derived cells began in Japan, only 10 years after the publication proof‐of concept article. In this exciting context, strategies to generate have evolved quickly, tending towards non‐invasive protocols sample somatic combined with “safer” reprogramming...
A human iPSC line was generated from exfoliated renal epithelial (ERE) cells of a patient affected with Congenital Heart Disease (CHD) and Laterality Defects carrying tshe variant p.R152H in the DAND5 gene. The transgene-free iPSCs were OSKM transcription factor using Sendai-virus reprogramming system. established had specific heterozygous alteration, stable karyotype, expressed pluripotency markers embryoid bodies that can differentiate towards three germ layers vitro. This offers useful...
Heart failure with preserved ejection fraction (HFpEF) represents a global health challenge, limited therapies proven to enhance patient outcomes. This makes the elucidation of disease mechanisms and identification novel potential therapeutic targets priority. Here, we performed RNA sequencing on ventricular myocardial biopsies from patients HFpEF, prospecting discover distinctive transcriptomic signatures. A total 306 differentially expressed mRNAs (DEG) 152 microRNAs (DEM) were identified...
A DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, transgene-free status analyzed confirmed. This cell can be exploited as control to better understand mechanisms involved DAND5-associated cardiac disease.