A5082832237- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Plant nutrient uptake and metabolism
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Bacterial Identification and Susceptibility Testing
- Advanced Biosensing Techniques and Applications
Indiana University
2025
Indiana University Bloomington
2022-2024
Significance How bacteria with multipartite genomes organize and segregate their DNA is poorly understood. Here, we investigate a prototypical genome in the plant pathogen Agrobacterium tumefaciens . We identify previously unappreciated interreplicon interactions: four replicons cluster through interactions at centromeres, two chromosomes, one circular linear, interact along replication arms. Our data suggest that these contacts play critical roles organization maintenance of genomes.
Abstract Research on chromosome organization and cell cycle progression in spherical bacteria, particularly Staphylococcus aureus , remains limited fragmented. In this study, we established a working model to investigate dynamics S. using Fluorescent Repressor-Operator System (FROS), which enabled precise localization of specific chromosomal loci. This approach revealed that the replication are not coupled, with cells exhibiting two segregated origins at start cycle. The has...
Abstract Structural maintenance of chromosomes (SMC) complexes organize genomes by extruding DNA loops, while replisomes duplicate entire chromosomes. These essential molecular machines must collide frequently in every cell cycle, yet how such collisions are resolved vivo remains poorly understood. Taking advantage the ability to load SMC at defined sites Bacillus subtilis genome, we engineered head-on and head-to-tail between replisome. Replisome progression was monitored marker frequency...
About 10% of sequenced bacteria have multiple replicons, also known as multipartite genomes. How these genomes are maintained is still poorly understood.
Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay as a sensitive way assess impact KCK tag on property DNA-binding proteins. Using Bacillus subtilis ParB example, show that, although no noticeable changes were detected by vivo fluorescence imaging and chromatin immunoprecipitation (ChIP) assays,...
Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added proteins for visualization. In this study, we employed