A5035309262- Wheat and Barley Genetics and Pathology
- Plant Disease Resistance and Genetics
- Genomics and Chromatin Dynamics
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Vector-borne infectious diseases
- Chromosomal and Genetic Variations
- Bacteriophages and microbial interactions
- Genetic Mapping and Diversity in Plants and Animals
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Plant Pathogens and Resistance
- DNA and Nucleic Acid Chemistry
- Plant Pathogenic Bacteria Studies
- Genetics and Plant Breeding
- Advanced biosensing and bioanalysis techniques
- Vector-Borne Animal Diseases
- Plant and Fungal Interactions Research
- Horticultural and Viticultural Research
- Plant tissue culture and regeneration
- Advanced Fluorescence Microscopy Techniques
- Nuts composition and effects
- Plant and fungal interactions
- Plant pathogens and resistance mechanisms
- Insect and Pesticide Research
Indiana University Bloomington
2020-2025
Memorial Sloan Kettering Cancer Center
2025
Indiana University
2021
Sichuan Agricultural University
2007-2015
University of Electronic Science and Technology of China
2007-2014
Bayerische Landesanstalt für Weinbau und Gartenbau
2000
Genome organization is important for DNA replication, gene expression, and chromosome segregation. In bacteria, two large families of proteins, nucleoid-associated proteins (NAPs) SMC complexes, play roles in organizing the genome. NAPs are highly abundant DNA-binding that can bend, wrap, bridge, compact DNA, while complexes load onto chromosome, translocate on extrude loops. Although capable traversing entire bound by various vivo, it unclear whether translocation influenced NAPs. this...
Significance How bacteria with multipartite genomes organize and segregate their DNA is poorly understood. Here, we investigate a prototypical genome in the plant pathogen Agrobacterium tumefaciens . We identify previously unappreciated interreplicon interactions: four replicons cluster through interactions at centromeres, two chromosomes, one circular linear, interact along replication arms. Our data suggest that these contacts play critical roles organization maintenance of genomes.
Abstract Structural maintenance of chromosomes (SMC) complexes organize genomes by extruding DNA loops, while replisomes duplicate entire chromosomes. These essential molecular machines must collide frequently in every cell cycle, yet how such collisions are resolved vivo remains poorly understood. Taking advantage the ability to load SMC at defined sites Bacillus subtilis genome, we engineered head-on and head-to-tail between replisome. Replisome progression was monitored marker frequency...
Multipartite bacterial genome organization can confer advantages including coordinated gene regulation and faster replication but is challenging to maintain. Agrobacterium tumefaciens lineages often contain a circular chromosome (Ch1), linear (Ch2), multiple plasmids. We previously observed that in some stocks of the lab model strain C58, Ch1 Ch2 were fused into dicentric chromosome. Here we analyzed natural isolates from French Collection for Plant-Associated Bacteria (CFBP) identified two...
ABSTRACT Histone-like nucleoid structuring (H-NS) and H-NS-like proteins serve as global gene silencers work with antagonistic transcriptional activators (counter-silencers) to properly coordinate the expression of virulence genes in pathogenic bacteria. In Brucella , MucR has been proposed a novel silencer, but direct experimental evidence is lacking. Here, we show that serves an silencer abortus encoding polar autotransporter adhesins BtaE BmaC, c-di-GMP-specific phosphodiesterase BpdB,...
Eleven weedy or wild species subspecies of the genus Secale L. were compared with a set cultivated rye accessions, based on inter-simple sequence repeat (ISSR) markers to analyze their phylogenetic relationships. A total 846 bands amplified from reactions using 12 screening primers, including 79 loci mean 10.1 alleles per locus. The number for each primer ranged 134, 70.5 primer. presence and distribution in different accessions demonstrate that rapid evolutionary trend microsatellite...
CN17 is a functional stay-green wheat variety that exhibits delayed leaf senescence and enhanced photosynthetic competence. To better understand these valuable traits, levels of chlorophyll b, soluble proteins, unsaturated fatty acids, other components were assayed. In addition, chloroplast ultrastructure, number, differences in gene expression between control variety, MY11, examined. By 21 d post-anthesis (DPA), leaves exhibited significantly higher maximal photochemical efficiency for...
In this study, a new disomic addition line, 12-5-2, with 44 chromosomes that was derived from BC 3 F 2 descendants of the hybridization between Triticum aestivum cv.CN19 and Aegilops biuncialis created reported.12-5-2 immune to both powdery mildew stripe rust has stable fertility.Fluorescence in situ C-banding revealed 12-5-2 1U b line (ADL1U ).The seed storage protein electrophoresis showed presented all high molecular weight glutenin subunits (7 + 8 12) CN19 were designated Ux...
About 10% of sequenced bacteria have multiple replicons, also known as multipartite genomes. How these genomes are maintained is still poorly understood.
RodZ of rod-shaped bacteria functions to link MreB filaments the Rod peptidoglycan (PG) synthase complex that moves circumferentially perpendicular long cell axis, creating hoop-like sidewall PG. Ovoid-shaped bacteria, such as Streptococcus pneumoniae (pneumococcus; Spn) lack MreB, use a different modality for peripheral PG elongation emanates from midcell dividing cells. Yet, S. encodes homolog similar in bacteria. We show here helix-turn-helix and transmembrane domains RodZ(Spn) are...
Abstract Wheat (Triticum aestivum) lines harboring the wheat-rye 1BL/1RS chromosomal translocation have become unpopular for genetic improvement as disease resistance genes carried on rye (Secale cereale) chromosome arm 1RS are no longer effective and yield advantage has only been seen in certain backgrounds. We developed several wheat with translocated from various inbreds. In this study, cultivars or inbred line L155 R12 were evaluated reaction to races CYR31 CYR32 of Puccinia striiformis...
Rye (Secale cereale L.) chromosome arm 1RS in the wheat (Triticum aestivum genetic background has potential significance for yield improvement without affecting its resistance to several diseases. A new cultivar, Chuannong17 (CN17), carrying wheat-rye 1BL/1RS translocated chromosome, exhibited “stay green” phenotypes. To determine behavior and physiological effect, MY11, CN17, BC 1 F , 2 populations of MY11/CN17 were grown normal growing seasons 2004–2005 2005–2006. Analysis photosynthetic...
The 1BL.1RS wheat-rye translocation contained in the Russian cultivar Aurora has been most widespread alien wheat-breeding programs all over world.However, following prevalence of new biotypes pathogens, disease-resistance genes this chromosome have overcome and consequently they eliminated modern programs.In paper, we report on 12 primary lines derived from crosses a Chinese high yield wheat cv.Mianyang 11 with three rye cultivars collected China.GISH, C-banding PCR techniques using...