A5054250281- Redox biology and oxidative stress
- Selenium in Biological Systems
- Vitamin C and Antioxidants Research
- Trace Elements in Health
- Cancer Immunotherapy and Biomarkers
- Glutathione Transferases and Polymorphisms
- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Organoselenium and organotellurium chemistry
- Photosynthetic Processes and Mechanisms
- Iron Metabolism and Disorders
- Ion channel regulation and function
- Sulfur Compounds in Biology
- Colorectal Cancer Treatments and Studies
- Ubiquitin and proteasome pathways
- Photodynamic Therapy Research Studies
- Pancreatic and Hepatic Oncology Research
- Photoreceptor and optogenetics research
- Cardiac electrophysiology and arrhythmias
- Electrochemical sensors and biosensors
- X-ray Diffraction in Crystallography
- Metabolomics and Mass Spectrometry Studies
- Traditional Chinese Medicine Analysis
- Crystallization and Solubility Studies
- Inflammatory Biomarkers in Disease Prognosis
Harbin Medical University
2015-2025
Third Affiliated Hospital of Harbin Medical University
2015-2025
Cornell University
2020-2025
Beijing Polytechnic
2024
Capital Medical University
2024
Heilongjiang Academy of Sciences
2023
Xinyang College of Agriculture and Forestry
2022
Fuzhou University
2018-2020
Georgia State University
2017-2020
Division of Chemistry
2019
Thioredoxin reductases (TRs) are important redox regulatory enzymes, which control the state of thioredoxins. Mammals have cytosolic and mitochondrial TRs, contain an essential selenocysteine residue reduce In addition, thioredoxin/glutathione reductase (TGR) was identified, is a fusion N-terminal glutaredoxin domain TR module. Here we show that TGR expressed at low levels in various tissues but accumulates testes after puberty. The protein particularly abundant elongating spermatids site...
Abstract Background Although numerous studies have indicated that activated pyroptosis can enhance the efficacy of antitumour therapy in several tumours, precise mechanism colorectal cancer (CRC) remains unclear. Methods Pyroptosis CRC cells treated with agents was assessed using various techniques, including Western blotting, lactate dehydrogenase release assay and microscopy analysis. To uncover epigenetic mechanisms regulate NLRP3, chromatin changes NLRP3 promoter histone modifications...
ABSTRACT Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal disease with poor prognosis and limited treatment options. While the majority of PDAC cases harbor KRAS mutations, approximately 8%–10% are wild‐type (KRAS‐WT). These KRAS‐WT tumors often contain actionable mutations gene fusions, making them more suitable for precision therapies. Identifying these molecular alterations crucial improving outcomes in this subset patients. This retrospective study involved 34 patients PDAC....
3016 Background: SYSA1801 is a MMAE antibody-drug conjugate (ADC) targeting claudin 18.2 (CLDN18.2), tight junction protein broadly expressed in gastric, pancreatic, and other solid tumors. CLDN18.2 has highly selective cell surface expression profile that limited to normal gastric mucosa, making it promising ADC therapeutic target. shown significant vitro vivo anti-tumor activity multiple lines patient-derived xenografts expressing CLDN18.2. Methods: This an open-label, multi-center, phase...
Mice homozygous for an allele encoding the selenocysteine (Sec) tRNA [Ser]Sec gene (Trsp) flanked by loxP sites were generated. Cre recombinase-dependent removal of Trsp in these mice was lethal to embryos. To investigate role mouse mammary epithelium, we deleted this using transgenic carrying recombinase under control tumor virus (MMTV) long terminal repeat or whey acidic protein promoter. While both promoters target expression MMTV-Cre is also expressed spleen and skin. Sec amounts reduced...
Human erythrocytes contain an unidentified plasma membrane redox system that can reduce extracellular monodehydroascorbate by using intracellular ascorbate (Asc) as electron donor. Here we show human erythrocyte membranes a cytochrome b561 (Cyt b561) and hypothesize it may be responsible for this activity. Of three evolutionarily closely related Cyts b561, immunoblots of showed only the duodenal (DCytb) isoform. DCytb was also found in guinea pig but not from mouse or rat. Mouse lost...
Thioredoxin reductase (TR) and thioredoxin (Trx) define a major cellular redox system that maintains cysteine residues in numerous proteins the reduced state. Both cytosolic (TR1 Trx1) mitochondrial (TR3 Trx2) enzymes are essential mammals, but function of is less understood. In this study, we characterized subcellular localization three TR3 forms generated by alternative first exon splicing differ their N-terminal sequences. Only one these resides mitochondria, whereas two other isoforms...
Thioredoxin/glutathione reductase (TGR) is a recently discovered member of the selenoprotein thioredoxin family in mammals. In contrast to two other mammalian reductases, it contains an N-terminal glutaredoxin domain and exhibits wide spectrum enzyme activities. To elucidate reaction mechanism regulation TGR, we prepared recombinant mouse TGR form as well various mutants individual domains this enzyme. Using these proteins, showed that could independently catalyze reactions normally...
Viperin is a radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication by converting cytidine triphosphate (CTP) into 3′-deoxy-3′,4′-didehydro-CTP and additional undefined mechanisms operating through its N- C-terminal domains. Here, we describe crystal structures of viperin bound to SAM analogue CTP or uridine (UTP) report kinetic parameters for viperin-catalyzed reactions with UTP as substrates. orients the C4′ hydrogen atom similarly abstraction 5′-deoxyadenosyl radical,...
Cytochromes b 561 are a family of transmembrane proteins found in most eukaryotic cells. Three evolutionarily closely related mammalian cytochromes (chromaffin granule cytochrome , duodenal and lysosomal ) were expressed Saccharomyces cerevisiae Δ fre1 fre2 mutant, which lacks almost all its plasma membrane ferrireductase activity, to study their ability reduce ferric iron (Fe 3+ ). The expression each these was able rescue the growth defect mutant cells iron‐deficient conditions, suggesting...
Many thioredoxin-fold proteins possess a conserved cis-proline located in their C-terminal portions. This residue, as well catalytic and resolving cysteines, is key functional group the active sites of these thiol−disulfide oxidoreductases. However, specific function proline poorly understood, some lack this residue. Herein, we found that mutation cis-proline, Pro75, human thioredoxin to serine, threonine, or alanine leads formation an Fe2-S2 cluster protein. Further mutagenesis studies...
Mammalian cytosolic and mitochondrial thioredoxin reductases are essential selenocysteine-containing enzymes that control functions. Thioredoxin/glutathione reductase (TGR) is a third member of this enzyme family. It has an additional glutaredoxin domain shows highest expression in testes. Herein, we found human several other mammalian TGR genes lack any AUG codons could function translation initiation. Although mouse rat TGRs have such codons, detected protein sequences upstream them by...
(Abstracted from Eur J Obstet Gynecol Reprod Biol 2024:302:104–110 Endometrial thickening without vaginal bleeding is frequently considered an indication for referral pathological evaluation, though gynecologists debate whether this necessary in women who are asymptomatic and postmenopausal. Current guidelines suggest biopsy when endometrial thickness reaches more than 4–5 mm individuals experience postmenopausal bleeding, even up to 15% of cancer cases occur bleeding.
Although the TNM staging system plays a critical role in guiding adjuvant chemotherapy for colorectal cancer (CRC), its precision risk stratification stage II and III CRC patients with proficient DNA mismatch repair (pMMR) remains limited. Therefore, precise predictive models research on postoperative treatments are crucial enhancing patient survival improving quality of life. This retrospective study analyzed 1051 pMMR who underwent radical resection were randomly assigned to training (n =...
ABSTRACT In children, hyper‐IgM syndrome type 1 (HIGM1) is a of severe antibody disorder, the pathogenesis which remains unclear. The diversity partially determined by alternative splicing (AS) in germline, mainly regulated RNA‐binding proteins, including Breast cancer amplified sequence 2 (Bcas2). However, effect Bcas2 on AS and production activated B cells, main immune cell unknown. To fill this gap, we created conditional knockout (cKO, cell‐specific AID‐Cre fl/fl ) mouse model performed...
Thioredoxin reductase 1 (TR1) is a key component in the thioredoxin system, one of major redox systems mammals that links NADPH and thiol-dependent processes. Mammalian TR1 genes are known to be regulated by alternative splicing. In this report, comparative genomic analyses were used identify characterize species-specific common forms mammalian genes. Six human isoforms identified derived from large number transcripts differed their N-terminal sequences. One isoform resulted exons located...
Fluorescent cofactors like flavins can be exploited to probe their local environment with spatial and temporal resolution. Although the fluorescence properties of oxidized two-electron-reduced states have been studied extensively, this is not case for one-electron-reduced state. Both neutral anionic semiquinones proven particularly challenging examine, as they are unstable in solution transient, short-lived species many catalytic cycles. Here, we report that nitronate monooxygenase (NMO)...
Garcinol is a natural product from the Garcinia Indica fruit and well-known as an antioxidant, anti-inflammatory, anticancer agent. However, understanding of its mechanism action still incomplete. It has been reported to be histone acetyltransferase (HAT) inhibitor. Here, we surprisingly found that garcinol potent deacetylase 11 (HDAC11) inhibitor (IC50 ∼ 5 μM in vitro with HPLC assay IC50 10 cellular SHMT2 fatty acylation assay), which comparable previously HDAC11 inhibitors. Additionally,...
Kinetic parameters (kcat and Km) derived from the Michaelis-Menten equation are widely used to characterize enzymes. kcat/Km is considered catalytic efficiency or substrate specificity of an enzyme toward its substrate. N-Myristoyltransferases (NMTs) catalyze N-terminal glycine myristoylation numerous eukaryotic proteins. Surprisingly, we find that in vitro human NMT1 can accept acetyl-CoA acetylation with kcat Km values similar myristoylation. However, when both myristoyl-CoA present...
Abstract As a free radical scavenger, and cofactor, ascorbate (ASC) is key player in the regulation of cellular redox processes. It involved responses to biotic abiotic stresses control enzyme activities metabolic reactions. Cytochromes (Cyts) b561 catalyze ASC-driven trans-membrane electron transport contribute ASC-mediated reactions subcellular compartments. Putative Cyts have been identified Arabidopsis (ecotype Columbia) on basis sequence similarity their mammalian counterparts. However,...
Ascorbate‐reducible cytochromes b561 (Cyts‐b561) are a class of intrinsic trans ‐membrane proteins. Tonoplast Cyt‐b561 (TCytb), one the four isoforms in Arabidopsis was localized to tonoplast. We demonstrate here that optical spectra, EPR spectra and redox potentials recombinant TCytb similar those well characterized bovine chromaffin granule Cyt‐b561. provide evidence for reduction ferric‐chelates by reduced TCytb. It is also shown capable electron transport from intracellular ascorbate...
In this study, we demonstrate a novel H2O2 activatable photosensitizer (compound 7) which contains diiodo distyryl boron dipyrromethene (BODIPY) core and an arylboronate group that quenches the excited state of BODIPY dye by photoinduced electron transfer (PET). The BODIPY-based is highly soluble remains nonaggregated in dimethyl sulfoxide (DMSO) as shown intense sharp Q-band absorption (707 nm). As expected, compound 7 exhibits negligible fluorescence emission singlet oxygen generation...