A5089356701- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Ultrasound Imaging and Elastography
- Ultrasound and Cavitation Phenomena
- Advanced MRI Techniques and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Ultrasonics and Acoustic Wave Propagation
- Advanced Fiber Optic Sensors
- Acute Ischemic Stroke Management
- Infective Endocarditis Diagnosis and Management
- Anesthesia and Neurotoxicity Research
- Photodynamic Therapy Research Studies
- Coronary Interventions and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Molecular Biology Techniques and Applications
- Nanoplatforms for cancer theranostics
- Spinal Cord Injury Research
- Spinal Dysraphism and Malformations
- Photonic and Optical Devices
- Optical Coherence Tomography Applications
- Thermoregulation and physiological responses
- Fluid Dynamics and Mixing
- Oral microbiology and periodontitis research
- Central Venous Catheters and Hemodialysis
- Cardiovascular and Diving-Related Complications
Indian Institute of Technology Gandhinagar
2020-2025
Johns Hopkins Medicine
2023
Johns Hopkins University
2023
Johns Hopkins Bayview Medical Center
2023
University of Cincinnati
2014-2020
Sabin Vaccine Institute
2016-2018
University of Rochester
2010-2017
Histotripsy, a form of therapeutic ultrasound that uses the mechanical action microbubble clouds for tissue ablation, is under development to treat chronic deep vein thrombosis (DVT). We hypothesize combining thrombolytic agents with histotripsy will enhance clot lysis. Recombinant plasminogen activator (rt-PA) and rt-PA-loaded echogenic liposomes entrain octafluoropropane microbubbles (OFP t-ELIP) were used in combination highly shocked pulses. Fully retracted porcine venous clots, similar...
Passive cavitation imaging (PCI) derived metrics can serve as surrogates for the outcome of bubble-mediated therapies. is limited by poor axial resolution and side lobe artifacts, particularly when algorithms such delay, sum integrate (DSI) beamforming are used. Methods to improve PCI performance remain an active research area given need balance with computational complexity. The current study evaluated pth root compression (prDSI) using in vitro vivo data collected insonation parameters...
Histotripsy is a non-invasive focused ultrasound therapy that liquifies tissue via bubble activity. Conventional imaging used in current clinical practice to monitor histotripsy. Developing surrogate metrics for successful treatment outcomes remains an unmet need. The goal of this work was twofold. First, we investigated whether histotripsy clouds detected with nonlinear (chirp-coded subharmonic and without Volterra filtering) can be assess the ablation zone vitro. Second, evaluated...
Echogenic liposomes loaded with the thrombolytic recombinant tissue-type plasminogen activator (rt-PA) are under development for treatment of ischemic stroke. These agents designed to co-encapsulate cavitation nuclei promote bubble activity in response ultrasound exposure, and enable localized delivery thrombolytic. Stable improves efficacy through enhanced fluid mixing. that encapsulate air-filled microbubbles nucleate scant stable 120 kHz intermittent have demonstrated equivalent rt-PA...
Abstract The development of adjuvant techniques to improve thrombolytic efficacy is important for advancing ischemic stroke therapy. We characterized octafluoropropane and recombinant tissue plasminogen activator (rt-PA)-loaded echogenic liposomes (OFP t-ELIP) using differential interference fluorescence microscopy, attenuation spectroscopy, electrozone sensing. loading rt-PA in OFP t-ELIP was assessed spectrophotometry. Further, it tested whether the agent shields against degradation by...
Sonodynamic therapy (SDT) is a promising alternative to photodynamic for achieving site-specific cytotoxic therapy. Porphyrin derivative molecules have been reported extensively in We previously shown that the glycosylation of porphyrin-based sonosensitizers can enhance their cellular uptake. However, sonodynamic potential these water-soluble glycosylated porphyrins has not investigated. In this study, we characterized response two porphyrin derivatives. Ultrasound (US) exposure was...
Purpose: Subharmonic intravascular ultrasound imaging (S‐IVUS) could visualize the adventitial vasa vasorum , but high pressure threshold required to incite subharmonic behavior in an contrast agent will compromise sensitivity—a trait that has hampered clinical use of S‐IVUS. The purpose this study was assess feasibility using coded‐chirp excitations improve sensitivity and axial resolution Methods: response Targestar‐p TM a commercial microbubble (UCA), (5%–20% fractional bandwidth) pulses...
Abstract Background Tension in the spinal cord is a trademark of tethered syndrome. Unfortunately, existing tests cannot quantify tension across bulk cord, making diagnostic evaluation stretch ambiguous. A potential non-destructive metric for ultrasound-derived shear wave velocity (SWV). The sensitive to tissue elasticity and boundary conditions including strain. We use term Ultrasound Tensography describe acoustic with SWV. Methods Our solution Tethered Assessment (TAUT) was utilized three...
Purpose: Subharmonic imaging is of interest for high frequency (>10 MHz) nonlinear imaging, because it can specifically detect the response ultrasound contrast agents (UCA).However, conventional UCA produce a weak subharmonic at frequencies, which limits sensitivity imaging.We hypothesized that modifying size distribution agent enhance its high-frequency response.The overall goal this study was to investigate size-manipulated populations determine range sizes strongest frequencies (in case,...
Abstract Infective endocarditis (IE) is associated with high morbidity and mortality rates. The predominant bacteria causing IE Staphylococcus aureus ( S. ), which can bind to existing thrombi on heart valves generate vegetations (biofilms). In this in vitro flow study, we evaluated sonobactericide as a novel strategy treat IE, using ultrasound an contrast agent or without other therapeutics. We developed model of biofilm human whole-blood clots infected patient-derived (infected clots)....
Limited work has been reported on the acoustic and physical characterization of protein-shelled UCAs. This study characterized bovine serum albumin (BSA)-shelled microbubbles filled with perfluorobutane gas, along SonoVue, a clinically approved contrast agent. Broadband attenuation spectroscopy was performed at room (23 ± 0.5 °C) physiological (37 temperatures over period 20 min for these agents. Three size distributions BSA-shelled microbubbles, mean sizes 1.86 μm (BSA1), 3.54 (BSA2), 4.24...
The current excitation strategy for harmonic and subharmonic imaging (HI SHI) uses short sine-bursts. However, alternate pulsing strategies may be useful enhancing nonlinear emissions from ultrasound contrast agents. goal of this study was to corroborate the hypothesis that chirp-coded can improve performance high-frequency HI SHI. A secondary understand mechanisms govern response agents sine-burst schemes. Numerical simulations acoustic measurements were conducted evaluate a commercial...
While performing surgical excision for breast cancer (lumpectomy), it is important to ensure a clear margin of normal tissue around the achieve complete resection. The current standard histopathology; however, time-consuming and labour-intensive requiring skilled personnel.We describe Hybrid Spectral-IRDx - combination previously reported tool with multimodal ultrasound NIR spectroscopy techniques. We show how this portable, cost-effective, minimal-contact could provide rapid diagnosis using...
Introduction Porcine thrombi are employed routinely in preclinical models of ischemic stroke. In this study, we examined the differential lytic susceptibility porcine and human whole blood clots with without use microbubbles ultrasound (US) as an adjuvant. Materials methods An vitro system equipped time-lapse microscopy was used to evaluate recombinant tissue-plasminogen activator (rt-PA) lysis same species or cross plasma. Human were treated rt-PA echo contrast agent, Definity®, exposed...
Imaging plaque microvasculature with contrast-enhanced intravascular ultrasound (IVUS) could help clinicians evaluate atherosclerosis and guide therapeutic interventions. In this study, we evaluated the performance of chirp-coded ultraharmonic imaging using a modified IVUS system (iLab™, Boston Scientific/Scimed) equipped clinically available peripheral coronary catheters. Flow phantoms perfused phospholipid-encapsulated contrast agent were visualized at 12 MHz 30 transmit frequencies....
The global pandemic of antibiotic resistance is an ever-burgeoning public health challenge, motivating the development adjunct bactericidal therapies. Nitric oxide (NO) a potent bioactive gas that induces variety therapeutic effects, including and biofilm dispersion properties. short half-life, high reactivity, rapid diffusivity NO make delivery challenging. goal this work was to characterize NO-loaded microbubbles (MB) stabilized with lipid shell assess feasibility antibacterial therapy