An outbreak of human illness caused by Mayaro (MAY) virus occurred in Belterra, Pará, Brazil the first half 1978. A total 55 cases were confirmed, 43 isolation and serology, 12 serology alone. The disease Belterra presented as a distinct clinical syndrome characterized fever, arthralgia exanthema. No fatalities could be attributed to MAY infection. Arthralgia, accompanied joint edema 20% cases, was very prominent sign which temporary incapacity many patients. Arthralgia present virtually all...

SUMMARY From December 1982 to March 1986 a group of 80 children between 0 and 3 years old who lived in the peripheral area Belém, Brazil, were followed up for episodes diarrhoea. A total 441 diarrhoeal recorded 36 (8·2%) associated with rotavirus. This agent was only pathogen 50% rotavirus-related acute diarrhoea, strains characterized by analysis RNA polyacrylamide gels. Forty-one belonged subgroup II (long pattern) five I. Reinfections rotavirus noted 12 involving either same or different...

Abstract The prevalence and potential zoonotic transmission of group C rotavirus (RVC) were examined by testing fecal samples collected from children during a longitudinal study that was carried out in the outskirts Belém, Brazil, December 1982 to March 1986. involved 30 who followed birth 3 years. Of 77 tested 29 children, 5 (6.5%) positive for human (4%) porcine RVC using nested PCR assay with primers specific VP6 gene or Southern hybridization probe both RVC. In addition, total 59...

Although viruses are well-established causes of acute gastroenteritis, few data on the circulation these pathogens in Porto Velho, state Rondônia, Brazil, available. Thus, faecal samples from hospitalised diarrhoeic children, under six years age, were collected and tested for presence norovirus (NoV), adenovirus (AdV) astrovirus (AstV) February 2010-February 2012. Specimens screened by reverse-transcription polymerase chain reaction found 10.7% (63/591) cases. NoV, AdV AstV detected 7.8%, 2%...

During July-August 1977, an outbreak of acute diarrhea occurred in unusually isolated population, the Tiriyó Indians, who live north Pará, Brazil, near border with Surinam. Diarrhea was reported by 157 (70%) 224 Indians living village during epidemic. There one fatal case a year old child. Rotavirus detected electron microscopy fecal specimen collected from diarrhea. Seroconversions were noted 127 out 168 (75.6%) paired serum samples tested for rotavirus antibody...

Picobirnavirus (PBV) belongs to the family Picobirnaviridae . Picobirnaviruses contain a bisegmented dsRNA genome that is non-enveloped. A total of 85 pooled faecal samples were collected from poultry 37 farms Metropolitan Mesoregion Belém (MMB), Pará state, Brazil. The viral RNA each sample was analysed by PAGE and reverse transcriptase PCR (RT-PCR). For county affected, at least one positive selected, cloned sequenced. showed positivity 15.3 % (13/85) 49.4 (42/85) RT-PCR. Sequencing these...

A total of 614 fecal specimens were obtained during a survey for rotavirus infection conducted between May 1996 and 1998 among 437 newborns admitted to special care nurseries at public hospital in the urban area Belém, Brazil. Routine stool samples taken weekly from all babies up age 28 days. Overall, 51 (11.7%) neonates excreted rotaviruses while hospital, whom 42 (82.3%) developed asymptomatic nosocomial infection; was also proved five nine patients with diarrhea. Three distinct RNA...

A human rotavirus strain (NB-150) was detected in stool samples from a neonate hospitalized for mild/moderate community-acquired diarrhoea. This baby lived the outskirts of Belém, Brazil, under poor sanitation conditions. The NB-150 displayed typical long electrophoretic pattern with 11 gene segments. It had two VP7 alleles, G1 and G4, belonged to VP6 subgroup II. close relatedness rotaviruses shown alleles: (96.9-100 % similarity at amino acid level) G4 (97.1-100 level). As VP6, 95.1-97.5...

Group D rotaviruses (RVs-D) have been documented in birds and, while they may be common these animals, few molecular studies are available for this specific group. In study, primers the gene that encodes RVs-D VP6 protein were designed and used a reverse transcription polymerase chain reaction (RT-PCR). Thirty pools of samples tested by polyacrylamide gel electrophoresis (PAGE) yielding 30% (9/30) positivity. These subjected subsequently to RT-PCR, with 53% (16/30) positivity rate. The...

Norovirus (NoV), sapovirus (SaV) and human astrovirus (HAstV) are viral pathogens that associated with outbreaks sporadic cases of gastroenteritis. However, little is known about the occurrence these in relatively isolated communities, such as remnants African-descendant villages (“Quilombola”). The objective this study was frequency determination viruses children under 10 years, without gastroenteritis, from a “Quilombola” Community, Northern Brazil. A total 159 stool samples were obtained...

The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, the post‐rotavirus vaccination period, particularly with regard emerging, increasingly prevalent G12P[8] genotype. A total 488 fecal specimens from diarrheic and non‐diarrheic children were obtained between January December 2012. RVA detection was initially performed using enzyme‐linked immunosorbent assay (ELISA) method, followed by reverse‐transcription polymerase...

Enteric adenovirus (AdV), sapovirus (SaV), and human astrovirus (HAstV) are important pathogens involved in the gastroenteritis etiology. In this study, a total of 219 fecal samples sera were collected from children hospitalized for acute (AGE) two large pediatric hospitals Belém, March 2012 to April 2015. The analyzed by polymerase chain reaction (PCR) AdV HAstV (astrovirus) detection, Nested‐PCR qPCR SaV detection. was detected 50.2% (110/219) cases, with 42.7% (47/110) being sequenced...

Between January 1979 and December 1980, rotaviruses were detected in faecal samples from 122 (33.1%) of 369 diarrhoeic children less than six years old, living Belém, Brazil. In 55 (45.1%) the rotavirus-positive specimens, no bacteria or parasite associated with gastro-enteritis was found. Salmonella typhimurium, Shigella Escherichia coli found 92 (35%) 263 specimens examined for bacterial pathogens. Rotaviruses readily throughout year, which may indicate seasonal pattern incidence Belém...

Abstract Worldwide human astroviruses (HAstV) have increasingly been recognized as causative agents of viral gastroenteritis, mainly in infants and young children. The aim this study was to assess the epidemiology genotype diversity HAstVs detected children who participated a trial Belém, Brazil with rhesus reassortant rotavirus vaccine tetravalent (RRV‐TV). From April/1990 August/1992, 624 diarrheic stool samples were tested by enzyme immunoassay (EIA) for HAstV, positive rate 4.0%. Reverse...

Noroviruses (NoVs) are a common cause of acute gastroenteritis (AGE) and until now, little is known about its ability to spread outside the gut.We aim investigate role NoVs causing viremia in children hospitalized for AGE, as well correlate presence RNA serum with clinical severity stool viral load.Paired samples were collected from 85 pediatric patients under 6 years AGE March September 2012 Belém, Brazil. Enzyme-linked immunosorbent assay (EIA) reverse transcription quantitative PCR...

Abstract Noroviruses are the leading cause of epidemic, non‐bacterial outbreaks acute gastroenteritis, and also a major sporadic gastroenteritis in infants. The aim present study was to identify norovirus infections children not infected by rotavirus admitted hospital for Belém. A total 348 fecal specimens were obtained from with diarrhea aged less than 5 years, all whom had tested negative rotavirus, between May 2008 April 2010. Fecal samples screened antigen using enzyme‐immunoassay (EIA)....

Worldwide, norovirus (NoV) is a major cause of acute gastroenteritis (AGE) responsible for pandemics every ~3 years, and over 200,000 deaths per year, with the majority in children from developing countries. We investigate incidence NoV hospitalized AGE Belém, Pará, Brazil, also correlated viral RNA levels their blood stool clinical severity. For this purpose, paired serum samples were collected 445 pediatric patients, ≤9 years between March 2012 June 2015. Enzyme-linked immunosorbent assay...

A chronologically comprehensive 30-year study was conducted that involved children living in Belém, the Amazon region of Northern Brazil, who participated eight different studies from October 1982 to April 2011. The were followed either community or health units and hospitals order identify norovirus genotypes infections during this time. total 2,520 fecal specimens obtained subjected RT-PCR nucleotide sequencing for regions A, B, C, D P2 viral genome. An overall positivity 16.9% (n = 426)...