A5086942425- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Hepatitis Viruses Studies and Epidemiology
- Respiratory viral infections research
- Virus-based gene therapy research
- Child Nutrition and Water Access
- Animal Disease Management and Epidemiology
- Congenital Anomalies and Fetal Surgery
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Infant Nutrition and Health
- Genetic diversity and population structure
- Animal Genetics and Reproduction
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Plant Taxonomy and Phylogenetics
- Plant Diversity and Evolution
- Gut microbiota and health
- Insect and Pesticide Research
- Medicinal Plants and Bioactive Compounds
- TiO2 Photocatalysis and Solar Cells
- Body Composition Measurement Techniques
- Water Quality Monitoring and Analysis
- COVID-19 Impact on Reproduction
Instituto Evandro Chagas
2016-2025
Ministério da Saúde
2015-2025
Universidade Federal do Rio Grande do Sul
2023-2024
Ministério da Saúde
2005-2024
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2022
Universidade Católica Dom Bosco
2022
Genotype G3P[8] of rotavirus A (RVA) is detected worldwide, usually associated with Wa-like constellation and exhibiting a long RNA migration pattern. More recently, novel inter-genogroup, reassortant variant short electropherotype, has emerged in Asia, Oceania Europe, denoting an overall potential unusual strains. During RVA surveillance Brazil, strains were found displaying electropherotype pattern, which had not been before this region. This study aims to characterize the complete genome...
Abstract Rotavirus A (RVA) is the most common cause of severe acute gastroenteritis in infants and young children worldwide, causing 453,000 deaths annually. In Brazil, frequent genotype identified was G1 during almost three decades pre‐vaccination period; however, after anti‐rotavirus vaccine introduction, there a predominance G2 genotype. The aim this study to determine G P genotypes rotaviruses isolated from under 5 years age with Northern region discuss emergence G3P[6] total 783 stool...
A human rotavirus strain (NB-150) was detected in stool samples from a neonate hospitalized for mild/moderate community-acquired diarrhoea. This baby lived the outskirts of Belém, Brazil, under poor sanitation conditions. The NB-150 displayed typical long electrophoretic pattern with 11 gene segments. It had two VP7 alleles, G1 and G4, belonged to VP6 subgroup II. close relatedness rotaviruses shown alleles: (96.9-100 % similarity at amino acid level) G4 (97.1-100 level). As VP6, 95.1-97.5...
Norovirus (NoV), sapovirus (SaV) and human astrovirus (HAstV) are viral pathogens that associated with outbreaks sporadic cases of gastroenteritis. However, little is known about the occurrence these in relatively isolated communities, such as remnants African-descendant villages (“Quilombola”). The objective this study was frequency determination viruses children under 10 years, without gastroenteritis, from a “Quilombola” Community, Northern Brazil. A total 159 stool samples were obtained...
The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, the post‐rotavirus vaccination period, particularly with regard emerging, increasingly prevalent G12P[8] genotype. A total 488 fecal specimens from diarrheic and non‐diarrheic children were obtained between January December 2012. RVA detection was initially performed using enzyme‐linked immunosorbent assay (ELISA) method, followed by reverse‐transcription polymerase...
Acute gastroenteritis (AG) is a major illness in early childhood. Recent studies suggest potential association between human bocavirus (HBoV) and AG. HBoV, non-enveloped virus with single-strand DNA genome, belongs to the Parvoviridae family. This study aimed describe frequency of HBoV Northern Brazil using samples from patients AG collected 2017 2022. Fecal obtained viral surveillance network at Evandro Chagas Institute (IEC) were analyzed. suspensions (20%) prepared, genome was extracted....
We analyzed 364 children symptomatic or asymptomatic for respiratory symptoms, aged 0.1 month-17 years, selected from primary healthcare units of different municipalities Rondônia June 2021 to September 2022. Data were collected medical and electronic records epidemiological characterization. The positive cohort (n = 96) was quantified using a real-time (RT) qPCR sequenced by next-generation sequencing. Whole-genome sequences obtained, SARS-CoV-2 strains classified the Pango system, maximum...
It is important to understand which bacterial taxa are most abundant during SARS-CoV-2 infection and promote mitigation strategies for conditions subsequent infection. Nasopharyngeal swab samples were collected from patients infected with their family contacts (uninfected asymptomatic) the outbreak of P.1 variant in Parintins, Amazonas–Brazil, March 2021. The investigated by a shotgun sequencing metagenomic approach using NextSeq 500 Illumina® system. stratified according presence or absence...
Noroviruses (NoVs) are a common cause of acute gastroenteritis (AGE) and until now, little is known about its ability to spread outside the gut.We aim investigate role NoVs causing viremia in children hospitalized for AGE, as well correlate presence RNA serum with clinical severity stool viral load.Paired samples were collected from 85 pediatric patients under 6 years AGE March September 2012 Belém, Brazil. Enzyme-linked immunosorbent assay (EIA) reverse transcription quantitative PCR...
Abstract Rotaviruses (RVs) are the main cause of acute viral gastroenteritis in both humans and young animals various species such as calves, horses, pigs, dogs, cats, birds. The genetic diversity RVs is related to a variety evolutionary mechanisms, including point mutation, genome reassortment. objective this study was characterize molecularly genes that encode structural nonstructural proteins unusual RV strains. clinical specimens selected for were obtained from children newborn with...
Norovirus (NoV) is a major cause of acute gastroenteritis (AGE) worldwide, especially in children under five years. Studies involving the detection and molecular characterisation NoV have been performed Brazil, demonstrating its importance as an etiological agent AGE.The objectives this study were to investigate frequency human genotype strains isolated from 0-14-year-old patients AGE Manaus, over period two years.A total 426 faecal samples collected between January 2010 December 2011. All...
Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis (AGE) outbreaks worldwide, with infections reported in semiclosed environments, particularly hospitals and nursing homes. Astrovirus (HAstV) prevalent especially developing countries. We aimed to determine the prevalence, spatial distribution, genetic diversity NoV HAstV children under 5 years age Rio Branco city, Acre State, Amazon Region, Brazil. Stool samples from (n = 240) without 248) AGE were collected January...
Abstract Climate changes and associated glacial interglacial cycles during the Quaternary strongly influenced evolutionary history of countless number species. Subtropical highland grasslands (SHG) in southern South America constitute a distinct vegetation type with high diversity endemism rates. The most recent common ancestor genus Petunia (Solanaceae) originated lowland grasslands, some lineages secondarily migrated to SHG. Here, we describe an SHG species, P. altiplana, distributed...
Noroviruses (NoVs) are important cause of gastroenteritis in humans worldwide. Genotype GII.4 is responsible for the majority outbreaks reported to date. This study describes, first time Brazil, circulation NoV variant Sydney 2012 faecal samples collected from children aged less than or equal eight years Rio Branco, state Acre, northern during July-September 2012.
inflammaging is a chronic, sterile, low-grade inflammation that develops with advanced age in the absence of overt infection and may contribute to pathophysiology sarcopenia, progressive generalized skeletal muscle disorder. Furthermore, series biomarkers linked sarcopenia occurrence have emerged. To aid diagnostic treatment strategies for low mass other related conditions, objective this work was investigate potential associated appendicular lean community-dwelling older women. This...
Abstract Closely related and young species often show high morphological similarity, challenging their identification correct assignment. Molecular markers integrative approaches have contributed to solving many taxonomic uncertainties. In this study, we evaluated the genetic variability ecological features of Petunia guarapuavensis scheideana, over which there is a debate. Both are endemic rare, distributed in subtropical highland grasslands southern South America. We based our analyses on...
Abstract Aims The present study aimed to use a conventional and metagenomic approach investigate the microbiological diversity of water bodies in network drainage channels rivers located central area city Belém, northern Brazil, which is considered one largest cities Brazilian Amazon. Methods results In eight analyzed points, both bacterial viral indicators environmental contamination—physical–chemical metals—were assessed. resistance genes, drug mechanisms, viability environment were also A...
Noroviruses are highly infectious, genetically diverse viruses. Global outbreaks occur frequently, making molecular surveillance important for infection monitoring. This cross-sectional descriptive study aimed to monitor cases of norovirus gastroenteritis in the Brazilian Amazon. Fecal samples were tested by immunoenzymatic assay, RT-PCR and genetic sequencing ORF1/ORF2 protease regions. Bayesian inference with a clock was employed construct phylogeny. The prevalence 25.8%, higher positivity...
Several technological approaches have been used to develop vaccines against COVID-19, including those based on inactivated viruses, viral vectors, and mRNA. This study aimed monitor the maintenance of anti-SARS-CoV-2 antibodies in individuals from Brazil according primary vaccination regimen, as follows: BNT162b2 (group 1; 22) ChAdOx1 2; 18). Everyone received first booster while second CoronaVac, Ad26.COV2.S, or BNT162b2. Blood samples were collected 2021 2023 analyze specific RBD (ELISA)...
During June 1997-June 1999 rotavirus infection was screened in infants aged up to 2 years and hospitalised with acute diarrhoea São Luís, Northeastern Brazil. Altogether, 128 stool samples were collected from diarrhoeic patients additional 122 faecal specimens age temporal matched inpatients without obtained; positivity rates for these groups 32.0% (41/128) 9.8% (12/122), respectively (p < 0.001). Both electropherotyping serotyping could be performed 42 (79.2%) of the 53 rotavirus-positive...
The monovalent human rotavirus (RV) vaccine, RIX4414 (Rotarix™, GlaxoSmithKline Biologicals) was introduced into Brazil's Expanded Program on Immunization in March 2006. One year after vaccine introduction, the G2P[4] strain found to be predominant, with an apparent extinction of many non‐G2 strains. This study investigated diversity circulating strains three years following introduction. Between May 2008 and 2011, stool samples were collected from children aged ≥12 weeks who hospitalized...