A5005280576- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Malaria Research and Control
- Plant Virus Research Studies
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- Zoonotic diseases and public health
- Virology and Viral Diseases
- Infectious Encephalopathies and Encephalitis
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Indigenous Health and Education
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Primate Behavior and Ecology
- Testicular diseases and treatments
- Rural Development and Agriculture
- Genomics and Phylogenetic Studies
- Cervical Cancer and HPV Research
- Agricultural and Food Sciences
- Parasites and Host Interactions
- Poxvirus research and outbreaks
Instituto Evandro Chagas
2016-2025
Ministério da Saúde
2013-2024
Universidade do Estado do Pará
2009-2023
Ministry of Health of the Russian Federation
2023
Secretaria da Saúde
2012
Governo do Estado de Mato Grosso
2010
Abstract Zika virus (ZIKV) has recently caused a pandemic disease, and many cases of ZIKV infection in pregnant women resulted abortion, stillbirth, deaths congenital defects including microcephaly, which now been proposed as syndrome. This study aimed to investigate the situ immune response profile mechanisms neuronal cell damage fatal microcephaly cases. Brain tissue samples were collected from 15 cases, 10 microcephalic ZIKV-positive neonates with outcome five neonatal control...
Abstract In February 2008, a Mayaro fever virus (MAYV) outbreak occurred in settlement Santa Barbara municipality, northern Brazil. Patients had rash, fever, and severe arthralgia lasting up to 7 days. Immunoglobulin M against MAYV was detected by ELISA 36 persons; 3 isolates sequenced were characterized as genotype D.
Introduction A sero-epidemiological survey was undertaken to detect the circulation of arboviruses in free-living non-human primates. Methods Blood samples were obtained from 16 primates (13 Sapajus spp. and three Alouatta caraya) that captured using terrestrial traps anesthetic darts woodland regions municipalities Campo Grande, Aquidauana, Jardim, Miranda Corumbá State Mato Grosso do Sul, Brazil. The sent Instituto Evandro Chagas (IEC) Ananindeua, Pará, Brazil, antibodies against 19...
ABSTRACT The Brazilian western Amazon region is currently experiencing its largest laboratory confirmed Oropouche virus (OROV) outbreak, with nearly 6,000 reported cases in the states of Amazonas (AM), Acre (AC), Rondônia (RO), and Roraima (RR), between August 2022 March 2024. Here, we sequenced analyzed 382 full-length OROV genomes from human samples collected 2024 all four states, aiming to trace origin genetic evolution leading current outbreak. Genomic analyses revealed that recent...
Oropouche fever is caused by the virus (OROV; Bunyaviridae, Orthobunyavirus), one of most frequent arboviruses that infect humans in Brazilian Amazon. This year, an OROV outbreak was identified Brazil, and its vertical transmission reported, which associated with fetal death microcephaly. We describe clinical manifestations three cases congenital infection confirmed serology (OROV-IgM) mother-newborn binomial. One newborns died, post-mortem molecular analysis using real-time RT-qPCR genome...
A Brazilian ranch worker with encephalitis and flaccid paralysis was evaluated in the regional Acute Encephalitis Syndromic Surveillance Program. This first patient who met Centers for Disease Control Prevention (CDC) confirmation criteria West Nile virus disease. Owing to overlapping of neurological manifestations attributable several viral infections central nervous system, this report exemplifies importance human acute surveillance. The syndromic approach cases may enable early detection...
Serological evidence of West Nile virus (WNV) infection has been reported in different regions Brazil from equine and human hosts but the had never isolated country.We sought to identify viral etiology encephalitis Espírito Santo state.We performed culture C6/36 cells, molecular detection WNV genome, histopathology immunohistochemistry horse cerebral tissue. We also carried out sequencing, phylogenetic analysis clock.Histopathologic tissue showed injury related was confirmed by...
Abstract An Oropouche virus strain was isolated from a novel host (Callithrix sp.) in Arinos, Minas Gerais State, southeastern Brazil. The identified by complement fixation test and confirmed reverse transcription–polymerase chain reaction. Phylogenetic analysis this as genotype III isolate previously recognized only Panama.
Abstract Oropouche fever has reemerged in Parauapebas and Porto de Moz municipalities, Pará State, Brazil. Serologic analysis (immunoglobulin M–ELISA) virus isolation confirmed (OROV) both municipalities. Nucleotide sequencing of 2 OROV isolates from each location indicated genotypes I (Parauapebas) II (Porto Moz)
Zika virus (ZIKV) has caused substantial concern worldwide owing to its association with severe birth defects, such as microcephaly and other congenital malformations. Inflammasomes, i.e., multi-protein complexes that induce inflammation pyroptosis, are predicted contribute the immune response this flavivirus. Accordingly, in study, situ inflammasome was evaluated fatal cases of ZIKV-linked microcephaly. Brain tissue samples were collected from eight babies, including four ZIKV-positive...
Abstract We describe two neurological cases of Oropouche virus infection in northern Brazil, where the is endemic but neglected as a pathogen. This study reiterates necessity developing protocols for diagnosing infections and training medical personnel to recognize pathogenicity infections.
INTRODUCTION: From February-September 2010, seroepidemiological surveys were conducted on non-human primates and transmitter vector capture was used to investigate the possible circulation of arboviruses in municipalities Bonito, Campo Grande, Jardim, State Mato Grosso do Sul, Brazil. METHODS: A total 65 from wild captivity used, potential vectors captured using Castro dip nets. Serum samples tested at Instituto Evandro Chagas, Pará, hemagglutination inhibition test detect antibodies against...
The authors describe the baseline malaria prevalence and arbovirus seroprevalence among 467 subjects in an ongoing cohort study rural Amazonia. Most (72.2%) reported one or more previous episodes of malaria, 15.6% had been hospitalized for but only 3.6% individuals five years older parasites detected by microscopy (10 with Plasmodium vivax 4 P. falciparum). Antibodies to Alphavirus, Orthobunyavirus, and/or Flavivirus were hemagglutination inhibition (HI) 42.6% aged older, a higher...
The state of Pará encompasses 26% Brazilian Amazon where an enormous diversity arboviruses has been found. This study aimed to assess the prevalence and distribution hemagglutination-inhibition antibodies against antigens six Flavivirus (yellow fever virus, Ilheus Saint Louis encephalitis Cacipacore Bussuquara virus Rocio virus) in water buffaloes state, Brazil. these farm animals is important determine circulating arboviruses.All investigated were detected species studied our results...
Introduction: The recent Zika virus(ZIKV) epidemic in Brazil was characterized by a range of different clinical presentations, particularly microcephaly, Guillain-Barré syndrome, and death. In this context, we determined the causal relationship between fatal microcephaly cases ZIKV infection. Methods: Twelve neonates, whose mothers were infected with during pregnancy, examined; included nine neonatal deaths due to one miscarriage, two stillbirths. Tissue samples obtained from all at necropsy...
Because of its ecological characteristics, the Caxiuanã National Forest (FLONA) is a potential area arbovirus circulation. The present study aimed to investigate occurrence transmission cycles at FLONA de Caxiuanã. Five field trips were performed capture mosquitoes and sylvatic vertebrates. For these vertebrates, we attempted viral isolation by cell monolayer inoculation from blood, hemagglutination inhibition further seroneutralization assays sera. mosquitoes, tests genome detection. A...
Oropouche fever is caused by the virus (OROV; Bunyaviridae, Orthobun-yavirus), one of most frequent arboviruses that infect humans in Brazilian Amazon. This year, an OROV outbreak was identified Brazil, and its vertical trans-mission reported, which associated with fetal death microcephaly. We describe clinical manifestations three cases congenital infec-tion confirmed serology (OROV-IgM) mother-newborn binomial. One newborns died, post-mortem molecular analysis using real-time RT-qPCR...
We describe evidence of circulation hantaviruses in the influence area Santarém-Cuiabá Highway (BR-163) Brazilian Amazon through prevalence specific antibodies against inhabitants living four municipalities this area: Novo Progresso (2.16%) and Trairão (4.37%), state Pará (PA), Gua-rantã do Norte (4.74%) Marcelândia (9.43%), Mato Grosso. also demonstrate ongoing association between Castelo dos Sonhos virus (CASV) hantavirus pulmonary syndrome (HPS) cases district (municipality Altamira, PA)...