A5055931855- Single-cell and spatial transcriptomics
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Environmental DNA in Biodiversity Studies
- Forensic and Genetic Research
- dental development and anomalies
Max Planck Institute for Evolutionary Anthropology
2019-2021
Abstract Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of states within these systems; however, approaches are needed directly measure lineage relationships. Here we establish iTracer, a recorder that combines reporter barcodes with inducible CRISPR–Cas9 scarring and is compatible single-cell spatial transcriptomics. We apply iTracer explore clonality dynamics during...
Diverse regions develop within cerebral organoids generated from human induced pluripotent stem cells (iPSCs), however it has been a challenge to understand the lineage dynamics associated with brain regionalization. Here we establish an inducible recording system that couples reporter barcodes, CRISPR/Cas9 scarring, and single-cell transcriptomics analyze relationships during organoid development. We infer fate-mapped whole phylogenies over scarring time course, reconstruct...
Induced pluripotent stem cells (iPSCs) from diverse humans offer the potential to study human functional variation in controlled culture environments. A portion of this originates an ancient admixture between modern and Neandertals, which introduced alleles that left a phenotypic legacy on individual today. Here, we show large iPSC repository harbors extensive Neandertal DNA, including contribute phenotypes diseases, encode hundreds amino acid changes, alter gene expression specific tissues....
ABSTRACT The human brain has changed dramatically since humans diverged from our closest living relatives, chimpanzees and the other great apes 1–5 . However, genetic developmental programs underlying this divergence are not fully understood 6–8 Here, we have analyzed stem cell-derived cerebral organoids using single-cell transcriptomics (scRNA-seq) accessible chromatin profiling (scATAC-seq) to explore gene regulatory changes that specific humans. We first analyze cell composition...