A5102976411- Neuroscience and Neuropharmacology Research
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Chemistry and Chemical Engineering
- ATP Synthase and ATPases Research
- Process Optimization and Integration
- Cancer Genomics and Diagnostics
- interferon and immune responses
- Vagus Nerve Stimulation Research
- Magnesium in Health and Disease
- Gut microbiota and health
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- Mitochondrial Function and Pathology
- Hormonal Regulation and Hypertension
- Immune cells in cancer
- Computational Drug Discovery Methods
- Influenza Virus Research Studies
- Tryptophan and brain disorders
University of Otago
2010-2024
Wellcome Sanger Institute
2024
Roche (Switzerland)
2024
ETH Zurich
2020-2024
University of California, San Diego
2018
Abstract Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human organ development. Single-cell transcriptomics enable highly resolved descriptions of states within these systems; however, approaches are needed directly measure lineage relationships. Here we establish iTracer, a recorder that combines reporter barcodes with inducible CRISPR–Cas9 scarring and is compatible single-cell spatial transcriptomics. We apply iTracer explore clonality dynamics during...
The epithelial sodium channel (ENaC) plays an important role in controlling Na + homeostasis, extracellular fluid volume, and blood pressure. Copper metabolism Murr1 domain-containing protein 1 (COMMD1) interacts with ENaC downregulates ENaC. COMMD1 belongs to the COMMD family consisting of COMMD1–10, all members share a C-terminal COMM domain. Here, we report that COMMD2–10 also ENaC, COMMD3 COMMD9 were selected for further study. Amiloride-sensitive current mammalian epithelia expressing...
Diverse regions develop within cerebral organoids generated from human induced pluripotent stem cells (iPSCs), however it has been a challenge to understand the lineage dynamics associated with brain regionalization. Here we establish an inducible recording system that couples reporter barcodes, CRISPR/Cas9 scarring, and single-cell transcriptomics analyze relationships during organoid development. We infer fate-mapped whole phylogenies over scarring time course, reconstruct...
Background The epithelial sodium channel (ENaC) is an integral component of the pathway for Na+ absorption in cells. ubiquitin ligases Nedd4 and Nedd4-2 bind to ENaC decrease its activity. Conversely, Serum- Glucocorticoid regulated Kinase-1 (SGK1), a downstream mediator aldosterone, increases This effect at least partly mediated by direct interaction between SGK Nedd4-2. binds both Nedd4-2, but it only able phosphorylate Phosphorylation reduces ability ENaC, due phosphorylated with 14-3-3...
Abstract Bispecific antibodies (biAbs) used in cancer immunotherapies rely on functional autologous T cells, which are often damaged and depleted patients with haematological malignancies other immunocompromised patients. The adoptive transfer of allogeneic cells from healthy donors can enhance the efficacy biAbs, but donor binding to host-cell antigens cause an unwanted alloreactive response. Here we show that engineered a T-cell receptor does not convert antigen into cluster...
The δ epithelial sodium channel (δENaC) is a proton-activated, sodium-selective, amiloride-sensitive ion in the ENaC/degenerin family of channels involved blood pressure regulation and mechanosensation. Other ENaC members are subject to ubiquitin modification leading internalization from cell surface, degradation channel. Here, we show that δENaC also modified by on three intracellular lysine residues. Absence these lysines abolished increased surface levels δENaC. Although HECT-domain...
Nonstructural protein 1 (NS1) is a multifunctional involved in preventing host–interferon response influenza A virus (IAV). Previous studies have indicated that NS1 also stimulates the translation of viral mRNA by binding to conserved sequences 5′-UTR. Additionally, binds poly(A) (PABP1) and eukaryotic initiation factor 4G (eIF4G). The interaction with 5′-UTR, PABP1, eIF4G has been suggested specifically enhance mRNAs. In contrast, we report does not directly bind indicating responsible for...