The structural organization of four microbial communities was analysed by a novel computer program, COMSTAT, which comprises ten features for quantifying three-dimensional biofilm image stacks. Monospecies biofilms each the bacteria, Pseudomonas putida, P. aureofaciens, fluorescens and aeruginosa, tagged with green fluorescent protein (GFP) were grown in flow chambers defined minimal medium as substrate. Analysis COMSTAT program variables describing structure – mean thickness, roughness,...

Abstract We consider the problem of performing interpretable classification in high-dimensional setting, which number features is very large and observations limited. This setting has been studied extensively chemometrics literature, more recently become commonplace biological medical applications. In this a traditional approach involves feature selection before classification. propose sparse discriminant analysis, method for linear analysis with sparseness criterion imposed such that are...

Combined modeling of pixel intensities and shape has proven to be a very robust widely applicable approach interpret images. As such the active appearance model (AAM) framework been applied wide variety problems within medical image analysis. This paper summarizes AAM applications medicine describes public domain implementation, namely flexible environment (FAME). We give guidelines for use this research platform, show that optimization techniques used renders it interactive applications. To...

ABSTRACT Four strains of Pseudomonas aeruginosa (wild type, Δ pilHIJK mutant, lasI and rpoS mutant) were genetically tagged with the green fluorescent protein, development flow chamber-grown biofilms by each them was investigated confocal laser scanning microscopy. The structural developments quantified computer program COMSTAT (A. Heydorn, A. T. Nielsen, M. Hentzer, C. Sternberg, Givskov, B. K. Ersbøll, S. Molin, Microbiology 146:2395-2407, 2000). Two key variables, average thickness...

The structural organization of microbial communities is influenced by many factors, e.g. nutrient composition, shear stress and temperature. This paper presents a general method for quantitative comparison biofilm structures assessment experimental reproducibility between independent experiments. By using novel computer program, COMSTAT, Pseudomonas aeruginosa an isogenic rpoS mutant were quantified. strains tagged with the green fluorescent protein (GFP) grown in flow chambers defined...

Applications in biotechnology such as gene expression analysis and image processing have led to a tremendous development of statistical methods with emphasis on reliable solutions severely underdetermined systems. Furthermore, interpretations are importance, meaning that the surplus inputs has been reduced concise model. At core this which augment standard linear models for regression, classification decomposition sparse obtained. This toolbox aims at making public available carefully...

Adaptor and scaffolding proteins determine the cellular targeting, spatial, thereby functional association of G protein-coupled seven-transmembrane receptors with co-receptors, transducers, downstream effectors adaptors post-signaling events such as receptor sequestration through interactions, mainly C-terminal intracellular tails receptors. A library from 59 representative members super family was probed glutathione S-transferase fusion for interactions four different adaptor previously...

In this paper, an unsupervised algorithm, called the Independent Histogram Pursuit (IHP), for segmenting dermatological lesions is proposed. The algorithm estimates a set of linear combinations image bands that enhance different structures embedded in image. particular, first estimated combination enhances contrast lesion to facilitate its segmentation. Given N-band image, corresponds line N dimensions, such separation between two main modes histogram obtained by projecting pixels onto line,...

From a set of longitudinal three-dimensional scans the same anatomical structure, authors have accurately modeled temporal shape and size changes using linear model. On total 31 computed tomography mandible from six patients, 14,851 semilandmarks are found automatically features new algorithm called geometry-constrained diffusion. The mapped into Procrustes space. Principal component analysis extracts one-dimensional subspace, which is used to construct growth worst case mean modeling error...

Multispectral imaging has been evaluated for characterization of the concentration a specific cartenoid pigment; astaxanthin. 59 fillets rainbow trout, Oncorhynchus mykiss, were filleted and imaged using rapid multispectral device quantitative analysis. The captures reflection properties in 19 distinct wavelength bands, prior to determination true samples ranged from 0.20 4.34 g per fish. A PLSR model was calibrated predict astaxanthin novel images, showed good results with RMSEP 0.27. For...

Cardiovascular diseases are globally a major cause of death. Magnesium deficiency is associated with several including cardiovascular diseases.To examine if low concentration magnesium in drinking water increased mortality and due to acute myocardial infarction stroke.A nationwide population-based cohort study using national health registries was used. A total 4,274,132 individuals aged 30 years or more were included. estimated by linkage residential addresses the period 2005-2016 quality...

The aim of the present study was to investigate dose‐effect relationship single doses 4 32 mg lornoxicam (LNX), a new nonsteroidal antiinflammatory drug belonging oxicam group, compared with placebo and 10 ketorolac (KET) in treatment pain after oral surgery. Also, it evaluate between adverse events different LNX. After surgical removal mandibular third molar, test medication taken when patients experienced at least moderate pain. medication, relief, intensity, any discomfort from were noted...

Abstract Motivation: Detection of protein spots in two-dimensional gel electrophoresis images (2-DE) is a very complex task and current approaches addressing this problem still suffer from significant shortcomings. When quantifying spot, most the software applications include lot background due to poor segmentation. Other use fixed window for task, resulting omission part or including quantification. The approach presented here segmentation quantification 2-DE aims minimize these problems....