A5053621898- Cellular transport and secretion
- Calcium signaling and nucleotide metabolism
- Autophagy in Disease and Therapy
- Graphene and Nanomaterials Applications
- Lipid Membrane Structure and Behavior
- Insect and Pesticide Research
- Molecular Junctions and Nanostructures
- Advanced biosensing and bioanalysis techniques
- interferon and immune responses
- Endoplasmic Reticulum Stress and Disease
- Lysosomal Storage Disorders Research
- Vector-borne infectious diseases
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Vectors
- Retinal Development and Disorders
- Mosquito-borne diseases and control
Osnabrück University
2020-2024
Helmholtz Centre for Infection Research
2019
The University of Texas Medical Branch at Galveston
2018
Article29 January 2019Open Access Source DataTransparent process The herpesviral antagonist m152 reveals differential activation of STING-dependent IRF and NF-κB signaling STING's dual role during MCMV infection Markus Stempel orcid.org/0000-0002-9240-3987 Viral Immune Modulation Research Group, Helmholtz Centre for Infection Research, Braunschweig, Germany Search more papers by this author Baca Chan Vanda Juranić Lisnić Center Proteomics, Faculty Medicine, University Rijeka, Croatia Astrid...
Endosomes and lysosomes harbor Rab5 Rab7 on their surface as key proteins involved in identity, biogenesis, fusion. Rab activation requires a guanine nucleotide exchange factor (GEF), which is Mon1-Ccz1 for Rab7. During endosome maturation, replaced by Rab7, though the underlying mechanism remains poorly understood. Here, we identify molecular determinants conversion vivo vitro, reconstitute with yeast metazoan proteins. We show (i) that an effector of Rab5, (ii) membrane-bound to directly...
Lysosomes are essential for cellular recycling, nutrient signaling, autophagy, and pathogenic bacteria viruses invasion. Lysosomal fusion is fundamental to cell survival requires HOPS, a conserved heterohexameric tethering complex. On the membranes be fused, HOPS binds small membrane-associated GTPases assembles SNAREs fusion, but how complex fulfills its function remained speculative. Here, we used cryo-electron microscopy reveal structure of HOPS. Unlike previously reported, significant...
Organelles of the endomembrane system contain Rab GTPases as identity markers. Their localization is determined by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). It remains largely unclear how these regulators are specifically targeted to organelles their activity regulated. Here, we focus on GAP Gyp7, which acts Rab7-like Ypt7 protein in yeast, surprisingly observe exclusively puncta proximal vacuole. Mistargeting Gyp7 vacuole strongly affects morphology,...
Insights into the conformational organization and dynamics of proteins complexes at membranes is essential for our mechanistic understanding numerous key biological processes. Here, we introduce graphene-induced energy transfer (GIET) to probe axial orientation arrested macromolecules lipid monolayers. Based on a calibrated distance-dependent efficiency within dynamic range 25 nm, analyzed involved in tethering fusion lysosome-like yeast vacuole. We observed that membrane-anchored Rab7-like...
Ehrlichia chaffeensis, the causative agent of human monocytotropic ehrlichiosis, secretes several effector proteins that bind host DNA to modulate gene expression. The tandem repeat protein 120 (TRP120), one largest proteins, has four nearly identical (TR) regions each consists 80 amino acids. In addition playing a role in ehrlichial binding and internalization, TRP120 translocates nucleus where it is thought function as transcription factor modulates However, sequence analysis does not...
Abstract Lysosomes are of central importance in cellular recycling, nutrient signaling 1,2 and endocytosis, tightly connected to autophagy 3 the invasion pathogenic bacteria viruses 1,4 . Lysosomal fusion events fundamental cell survival require HOPS, a conserved heterohexameric tethering complex 5,6 HOPS recognizes binds small membrane-associated GTPases on lysosomes organelles, assembles membrane bound SNAREs for 7,8 Through tethering, brings membranes close proximity each other...