A5064187602- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Marine Toxins and Detection Methods
- Vibrio bacteria research studies
- Photoreceptor and optogenetics research
- Marine and coastal plant biology
- Algal biology and biofuel production
- Radiation Shielding Materials Analysis
- Genomics and Phylogenetic Studies
- Smart Agriculture and AI
- Nuclear Physics and Applications
- Nitric Oxide and Endothelin Effects
- Biocrusts and Microbial Ecology
- Electron Spin Resonance Studies
- Biofuel production and bioconversion
- Magnetic and Electromagnetic Effects
- Bacteriophages and microbial interactions
- Methane Hydrates and Related Phenomena
- Radiation Detection and Scintillator Technologies
- Water Quality and Pollution Assessment
- Pharmacology and Obesity Treatment
- Synthesis and Biological Evaluation
- Blood Pressure and Hypertension Studies
- Pluripotent Stem Cells Research
Shimane University
2013-2024
National Defense Academy of Japan
2019
Yamaguchi University
2005-2018
Nagasaki University
2018
Kyushu University
2018
Kōchi University
2010-2012
Akebono (Japan)
2011-2012
Juntendo University Urayasu Hospital
2011
University of Tsukuba
2009-2010
Kyowa Kirin (Japan)
2009
N-Nitrosation of a model aromatic amine (2,3-diamino-naphthalene) by the N-nitrosating agent produced nitrite in acidic solution was inhibited polyphenol, chlorogenic acid, which is an ester caffeic acid quinic acid. Caffeic also N-nitrosation, but did not. 1,2-Benzenediols and 3,4-dihydroxybenzoic had inhibitory activities. Chlorogenic 1,2-benzenediols were able to scavenge stable free radical, 1,1-diphenyl-2-picrylhydrazyl. found be nitrated nitrite. The kinetic studies nitration observed...
The ciliate Paramecium bursaria harbors several hundred cells of the green-alga Chlorella sp. in their cytoplasm. Irrespective mutual relation between P. and symbiotic algae, both retain ability to grow without partner. They can easily reestablish endosymbiosis when put contact with each other. Consequently, is an excellent model for studying cell–cell interaction evolution eukaryotic through secondary different protists. Despite importance this organism, no genomic resources have been...
Reprogramming of somatic cells provides potential for the generation specific cell types, which could be a key step in study and treatment human diseases. In vitro reprogramming into pluripotent embryonic stem (ES) cell-like state has been reported by retroviral transduction murine fibroblasts using four transcription factors or through fusion cells. Here we show that mouse adult bone marrow mononuclear (BM MNCs) are competent as donor can reprogrammed ES We isolated BM MNCs (MEFs) from...
Summary The association of ciliate Paramecium bursaria with symbiotic Chlorella sp. is a mutualistic symbiosis. However, both the alga‐free paramecia and algae can still grow independently be reinfected experimentally by mixing them. Effects host's nutritional conditions against algal cell division density were examined during early reinfection. Transmission electron microscopy revealed that starts 24 h after P. , mother wall discarded from perialgal vacuole membrane, which encloses alga....
Abstract Protists, including ciliates retain crystals in their cytoplasm. However, functions and properties remain unclear. To comparatively analyze the of Paramecium bursaria , a ciliate, associated with without endosymbiotic Chlorella variabilis we investigated isolated using light microscope analyzed length solubility. A negligible number was found P. cells harboring symbiotic algae. The average crystal alga-free algae-reduced about 6.8 μm 14.4 μm, respectively. were spherical, whereas...
Endosymbiosis between symbiotic Chlorella and alga-free Paramecium bursaria cells can be induced by mixing them. To establish the endosymbiosis, algae must acquire temporary resistance to host lysosomal enzymes in digestive vacuoles (DVs). When isolated from alga-bearing paramecia are kept under a constant dark conditions for 24 h before with paramecia, almost all digested DVs. examine cause of algal acquisition enzymes, were light or without photosynthesis inhibitor...
Abstract Extant symbioses illustrate endosymbiosis is a driving force for evolution and diversification. In the ciliate Paramecium bursaria , endosymbiotic alga Chlorella variabilis in perialgal vacuole localizes beneath host cell cortex by adhesion between membrane mitochondria. We investigated whether mitochondria are also affected algal endosymbiosis. Transmission electron microscopy of cells showed fewer algae-bearing than that alga-free cells. To compare density distribution with or...
Abstract Paramecium bursaria is a ciliate species that has symbiotic relationship with Chlorella spp. This study aimed to elucidate the dynamics of digestive vacuole (DV) differentiation in P. , using yeast stained pH indicator. Previously, DV been classified into eight periods based on fixed-cell observations. However, understand behavior and physiology its natural state, it essential observe living cells. presented novel method Cornig® Cell-Tak™ immobilize cells, which enabled long-term...