Nonadherent tissue culture cell lines were established from normal bone marrow of a variety mouse strains. The possessed morphological and histochemical markers the basophil/mast contained committed stem cells for metachromatic cells. Their derivation their lack tumorigenicity despite long-term makes these potentially important studies mechanisms allergic reactions inflammation as well differentiation pathways involving this subset hematopoietic

Insulin-like growth factor (IGF)-1 plays crucial roles in control and rearrangements of the cytoskeleton. IGF-1 binds to receptor thereby induces autophosphorylation this at its tyrosine residues. The phosphorylation is thought initiate a cascade events. Although various signaling molecules have been identified, they appear interact with tyrosine-phosphorylated receptor. Here, we identified leukemia-associated Rho guanine nucleotide exchange (GEF) (LARG), which contains PSD-95/Dlg/ZO-1...

Reprogramming of somatic cells provides potential for the generation specific cell types, which could be a key step in study and treatment human diseases. In vitro reprogramming into pluripotent embryonic stem (ES) cell-like state has been reported by retroviral transduction murine fibroblasts using four transcription factors or through fusion cells. Here we show that mouse adult bone marrow mononuclear (BM MNCs) are competent as donor can reprogrammed ES We isolated BM MNCs (MEFs) from...

The use of induced pluripotent stem cells (iPSCs) is an exciting frontier in the study and treatment human diseases through generation specific cell types. Here we show derivation iPSCs from nonmobilized peripheral blood (PB) bone marrow (BM) mononuclear (MNCs) by retroviral transduction OCT3/4, SOX2, KLF4, c-MYC. PB- BM-derived were quite similar to embryonic with regard morphology, expression surface antigens pluripotency-associated transcription factors, global gene profiles,...

Complementary DNAs encoding a previously unidentified mouse Notch ligand and its human ortholog were isolated. The new contains signal sequence, DSL domain, eight epidermal growth factor-like repeats, transmembrane an intracellular region, all of which are characteristics members the Delta protein family. was therefore designated Delta-4. Several sequences in both extracellular regions shown to be conserved among vertebrate proteins. tissue distribution Delta-4 mRNA resembles that described...

The cellular endomembrane system requires the proper kinetic balance of synthesis and degradation its individual components, which is maintained in part by a specific membrane fusion apparatus. In this study, we describe molecular properties D12, was identified from mouse expression library. This C-terminal anchored protein has sequence similarity to both yeast soluble N-ethylmaleimide-sensitive factor attachment (SNAP) receptor (SNARE), Use1p/Slt1p, recently human syntaxin 18-binding...

Abstract Granulopoiesis was investigated in five patients with congenital neutropenia (CN) (one Kostmann type, four benign forms). In semisolid agar culture, the marrow cells of all produced normal numbers CFU‐c (colony‐forming unit‐culture). The size and classification colonies were normal. suspension culture vitro exogenous colony‐stimulating factor (CSF) generated from omental‐conditioned medium (OMCM), myeloid precursors could proliferate differentiate into polymorphonuclear neutrophils...

A case of lumbar metastasis a choriocarcinoma is presented.To present and review rare metastatic in the spine.Choriocarcinoma highly anaplastic malignancy derived from trophoblastic cells characterized by secretion human chorionic gonadotropin (hCG) early hematogenous metastasis. However, spine extremely rare. Although 2 cases and/or sacral vertebra have been reported, efficacy surgical treatment for spinal not yet known.The clinical course, radiologic features, pathology, outcome reported.A...

Abstract A cotyledonoid dissecting leiomyoma is categorized as a with an unusual growth pattern, which characterized by remarkable extrauterine bulbous in continuity myometrial component. 36‐year‐old patient was preoperatively diagnosed mature cystic teratoma of the left ovary, and according to MRI, tumor protruded from uterus into right broad ligament 10 cm diameter. She underwent laparoscopic surgery resect ovarian under ligament. The almost completely resected based on intraoperative...

Introduction Tonic immobility (TI) is fear-induced freezing that animals may undergo when confronted by a threat. It principally observed in prey species as defence mechanisms. In our preliminary research, we detected large inter-individual variations the frequency and duration of behavior among newly hatched domestic chicks (Gallus gallus). this study aim to identify copy number (CNVs) genome genetic candidates underlie behavioral plasticity fearful stimuli. Methods A total 110 were used...

An 87-year-old was referred for gynecologic evaluation of a lesion involving the left labia majus noted 3 years earlier. Fine-needle aspiration cytology revealed clusters with an acinous structure or glandular formation. The tumor appeared as cell linear arrangements. Histologic examination showed same morphologic findings scirrhus type primary breast carcinoma. Examinations breasts and axillary lymph nodes were normal. This disease diagnosed adenocarcinoma arising in mammary-like glands...

Bevacizumab maintenance therapy following platinum-based combination chemotherapy for metastatic, recurrent, or persistent cervical cancer is not recommended as standard therapy. This pilot study aimed to evaluate the efficacy and safety of bevacizumab contribution platinum-free interval subsequent advanced cancer.We retrospectively identified 115 patients with treated platinum-paclitaxel plus at 7 institutions between 2015 2020. The primary endpoints were overall survival (OS)...

A male factor is involved in 50% of infertile relationships. In particular, men with nonobstructive azoospermia cannot have their own biological children. The treatment microdissection testicular sperm extraction, but the success rate low, and new approaches for infertility are needed. Stem cells potential to differentiate into different functional cell types, discovery has given rise field regenerative medicine. We focus here on therapeutic stem rejuvenating fertility. Embryonic (ESCs) can...

Polymorphisms in the neurotransmitter-related genes can be a major source of behavioral variations. We searched for polymorphic sites chicken and identified two variable number tandem repeat (VNTR) loci encompassing paralog serotonin transporter gene (5-HTT). Both intronic VNTR were highly across breeds other Galliformes species, even though predominant alleles considerably different among breeds. One locus contained sequences complementary to conserved motif CCCTC-binding factor (CTCF)...

ABSTRACT We surveyed two polymorphic microsatellites in a candidate gene for fear response, monoamine oxidase A ( MAOA ), chicken. Two hundred and eighty chickens from five breeds (Nagoya, Mikawa, White Leghorn, Plymouth Rock, Rhode Island Red) were investigated. thymine (T) repeat an adenine (A) found on intron 4 CMin4T ) 9 CMin9A respectively. Nine alleles 127‐bp to 166‐bp six 184‐bp 198‐bp detected. 128‐bp allele was observed the Nagoya breed only, implying highly useful marker...