A5022006562- PI3K/AKT/mTOR signaling in cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cellular transport and secretion
- Chronic Lymphocytic Leukemia Research
- Cancer Mechanisms and Therapy
- Histone Deacetylase Inhibitors Research
- Phagocytosis and Immune Regulation
- Pancreatic function and diabetes
- Genetics, Bioinformatics, and Biomedical Research
- Hippo pathway signaling and YAP/TAZ
- Protein Kinase Regulation and GTPase Signaling
- Adenosine and Purinergic Signaling
- Marine Biology and Environmental Chemistry
- Platelet Disorders and Treatments
- Neuroendocrine Tumor Research Advances
- Enzyme function and inhibition
- Axon Guidance and Neuronal Signaling
- Peptidase Inhibition and Analysis
- Environmental Toxicology and Ecotoxicology
- Toxic Organic Pollutants Impact
- Galectins and Cancer Biology
Peter MacCallum Cancer Centre
2012-2018
University Medical Center of the Johannes Gutenberg University Mainz
2018
Johannes Gutenberg University Mainz
2018
Rudjer Boskovic Institute
2018
Monash University
2005-2009
Mutations in the gene encoding p110α subunit of PI3K (PIK3CA) that result enhanced activity are frequently observed human cancers. To better understand role mutant PIK3CA initiation or progression tumorigenesis, we generated mice which a mutation commonly detected cancers (the H1047R mutation) could be conditionally knocked into endogenous Pik3ca locus. Activation this mouse ovary revealed alone, Pik3caH1047R induced premalignant hyperplasia ovarian surface epithelium but no tumors....
Endosomal trafficking is regulated by the recruitment of effector proteins to phosphatidylinositol 3-phosphate [PtdIns(3)P] on early endosomes. At plasma membrane, phosphatidylinositol-(3,4)-bisphosphate [PtdIns(3,4)P2] binds pleckstrin homology (PH) domain-containing Akt and TAPP1. Type Ialpha inositol polyphosphate 4-phosphatase (4-phosphatase) dephosphorylates PtdIns(3,4)P2, forming PtdIns(3)P, but its subcellular localization unknown. We report here in quiescent cells, colocalized with...
PIK3CA, the gene coding for p110α subunit of phosphoinositide 3-kinase, is frequently mutated in a variety human tumors including breast cancers. To better understand role mutant PIK3CA initiation and/or progression cancer, we have generated mice with conditional knock-in common activating mutation, Pik3caH1047R, into one allele endogenous mammary gland. These developed ductal anaplasia and hyperplasia by 6 weeks age characterized multi-layering epithelial lining ducts expansion luminal...
The spatial activation of phosphoinositide 3-kinase (PI3-kinase) signaling at the axon growth cone generates phosphatidylinositol 3,4,5 trisphosphate (PtdIns(3,4,5)P 3 ), which localizes and facilitates Akt stimulates GSK-3β inactivation, promoting microtubule polymerization elongation. However, molecular mechanisms that govern down-regulation PtdIns(3,4,5)P remain undetermined. inositol polyphosphate 5-phosphatases (5-phosphatase) hydrolyze 5-position phosphate from 4,5 bisphosphate...
The in vitro tube formation assay with human umbilical vein endothelial cells (HUVEC) was applied to identify the extra- and intracellular sources of metabolic energy/ATP required for cell migration during initial stage microvascularization. Extracellularly, physiological energy-rich polymer, inorganic polyphosphate (polyP), as biomimetic amorphous calcium polyP microparticles (Ca-polyP-MP), is functioning a substrate ATP generation most likely via combined action alkaline phosphatase (ALP)...
Environmental contamination includes a mixture of organic substances that can have detrimental effects on marine organisms and should be evaluated in the quality risk assessment investigated areas. Marine areas selected for this study are protected area, mariculture shipyard an industrial area. Based toxicity seawater extracts these were classified as undisturbed reference area (S1), with low anthropogenic impact (S2), potentially endangered (S3) high (S4) respectively. The mixtures present...
Oncogenic mutations in PIK3CA lead to an increase intrinsic phosphoinositide kinase activity, but it is thought that increased access of PI3Kα (phosphoinositide 3-kinase α) its PM (plasma membrane) localized substrate also required for levels downstream PIP3/Akt [phosphoinositide-3,4,5-trisphosphate/also called PKB (protein B)] signalling. We have studied the subcellular localization wild-type and two most common oncogenic mutants cells maintained growth media, starved or stimulated using a...