A5030008899- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Spine and Intervertebral Disc Pathology
- Musculoskeletal pain and rehabilitation
- Glioma Diagnosis and Treatment
- Trace Elements in Health
- Amyotrophic Lateral Sclerosis Research
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Pain Management and Treatment
- Hedgehog Signaling Pathway Studies
- Child Nutrition and Feeding Issues
- RNA regulation and disease
- Developmental Biology and Gene Regulation
- Spinal Fractures and Fixation Techniques
- Neuroscience and Neuropharmacology Research
- FOXO transcription factor regulation
- Epigenetics and DNA Methylation
- Melanoma and MAPK Pathways
- Protein Tyrosine Phosphatases
- Medicinal Plant Pharmacodynamics Research
- Immunotherapy and Immune Responses
- Cognitive Science and Mapping
- Mesenchymal stem cell research
- Chemokine receptors and signaling
Tokuda Hospital
2024
Acibadem City Clinic
2024
Medical University of Sofia
2022
Forschungsinstitut für Mineralische und Metallische Werkstoffe Edelsteine/Edemetalle
2014-2017
National Institute of Emergency Medicine "Pirogov"
2009-2010
Medical University of Vienna
2005-2007
University Hospital St. Ivan Rilski
2007
MRC Laboratory of Molecular Biology
2006
University of Vienna
1996-2003
Abstract Self‐renewal and differentiation of CNS stem cells are regulated by still poorly understood cell–cell interactions. Notch is a well‐known cell surface protein that can promote both cycle progression mitotic arrest but the molecular mechanism controlling these opposite effects unknown. Here we demonstrate that, in cells, level active Notch1 determines cellular response. Specifically, low levels form proliferation whereas high lead to growth arrest. provide first evidence on function...
Little is known about the pathogenetic basis of characteristic symptoms in transmissible spongiform encephalopathies (TSEs) such as myoclonus and EEG hyperactivity. We investigated GABAergic system its subpopulations mice inoculated with experimental scrapie (ME7, RML, 22A strains) Creutzfeldt‐Jakob disease (CJD; Fujisaki strain), to study damage inhibitory neurons. Since recent studies have shown electrophysiological changes prion protein (PrP) knockout mice, we also studied lacking or...
There is a distinctive pattern of hippocampal involvement in Creutzfeldt-Jakob disease (CJD) and evidence for selective vulnerability GABAergic neurons experimental human prion disease. We studied hippocampus temporal cortex from CJD control autopsy brains surgical cryptogenic lobe epilepsy specimens distribution density parvalbumin (PV) calbindin-D28K (Cal) -positive that are subpopulations neurons. Pathology was evaluated semiquantitatively 8 regions 23 severity spongiform change,...
BACKGROUND Recently, isocitrate dehydrogenase 1 ( IDH1 ) was identified as a major participant in glioma pathogenesis. At present, the enzymatic activity of protein has been main topic investigating its physiological function, but signaling pathway allocation unsuccessful. Interestingly, proteins regulated by phosphoinositide 3‐kinase (PI3K)/Akt signaling, are among top downregulated genes gliomas associated with high percentage and IDH2 mutations. The aim this study to investigate...
Abstract BACKGROUND: Animal studies have shown cooperative contribution of the Ras/Raf/MAPK and PI3K/Akt/mTOR signaling pathways in glioblastoma formation. However, this joint action has not yet been confirmed human studies. METHODS: The expression Raf kinase inhibitory protein (RKIP) was examined 159 patients with high‐grade low‐grade gliomas correlated previously obtained data on activation signal transducer activator transcription 3 (STAT3), a downstream effector pathway. RESULTS: RKIP...
Abstract BACKGROUND: We recently demonstrated that 86% of the patients treated with peripheral nerve stimulation (PNS) for therapy-refractory sacroiliac joint (SIJ) pain were satisfied result after 1 year treatment. OBJECTIVE: To investigate long-term (up to 4 years) response rate this novel METHODS: Sixteen consecutive SIJ PNS and followed years in 3 patients, 6 2 patient. Quality life, pain, patient satisfaction assessed using Oswestry Disability Index 2.0, Visual Analog Scale (VAS),...
Between 1969 and 30 September 1995, 79 Austrian patients had Creutzfeldt-Jakob disease (CJD) diagnosed neuropathologically by necropsy or biopsy. The annual incidence has significantly increased in recent years (average 0.18 per million 1969-85, 0.67 1986-94; estimate for 1995: 1.5 million). Also, the percentage of with CJD over 70 at death until 1989 but is since decline. There no regional clustering, familial occurrence, recognised iatrogenic risk. One patient a 10 year history...
Fatal familial insomnia (FFI) is a unique hereditary prion disease with characteristic disturbances of sleep. We studied the serotonergic system in 8 FFI-affected subjects by immunohistochemistry for serotonin-synthesizing enzyme, tryptophan hydroxylase (TH). Quantification neurons median raphe nuclei showed no total neuronal loss FFI but substantial increase TH+ (∼62%) compared controls. Our data indicate an alteration that might represent functional substrate some typical symptoms FFI. Ann...
The chemokine receptor CXCR7 is found on glioma cells and glioma-associated vessels dependent upon its localisation tumour or endothelial the can mediate cell invasion angiogenesis. Its expression predicts survival in several types of cancers.We immunohistochemically studied ligand SDF1α a cohort 354 human patients with glioma. In an vivo model, we effect selective inhibition mean vascular density.Here show that either mutant isocitrate dehydrogenase (IDH) 1 podoplanin (PDPN), two proteins...
Aims: Prion diseases are generally characterized by pronounced neuronal loss. In particular, a subpopulation of inhibitory neurones, the expression calcium‐binding protein parvalbumin (PV), is selectively destroyed early in course human and experimental prion diseases. By contrast, nerve cells expressing calbindin D28k (CB), another protein, as well PV/CB coexpressing Purkinje cells, preserved. Methods: To evaluate, if PV CB may directly contribute to vulnerability or resistance against cell...
Sleep disorders increase with ageing. The serotonergic system has been linked sleep regulation. In fatal familial insomnia, a prion disease insomnia as one major clinical feature, we recently observed disturbance in the likely substrate of typical symptoms. Using immunohistochemistry for serotonin synthesizing enzyme, tryptophan hydroxylase, investigated median raphe nuclei (dorsal nucleus, superior central and obscurus nucleus) brains an older (n = 12; age range 62–84 years) younger group...
Epithelial to mesenchymal transition (EMT) describes the process of epithelium transdifferentiating into mesenchyme. EMT is a fundamental during embryonic development that also commonly occurs in glioblastoma, most frequent malignant brain tumor. has been observed multiple carcinomas outside including breast cancer, lung colon gastric cancer. centrally linked malignancy by promoting migration, invasion and metastasis formation. The mechanisms induction are not fully understood. Here we...
Epithelial to mesenchymal transition (EMT) describes the process of epithelium transdifferentiating into mesenchyme. EMT is a fundamental during embryonic development that also commonly occurs in glioblastoma, most frequent malignant brain tumor. has been observed multiple carcinomas outside including breast cancer, lung colon gastric cancer. centrally linked malignancy by promoting migration, invasion and metastasis formation. The mechanisms induction are not fully understood. Here we...
Lumbar spinal stenosis is a widespread condition that significantly affects the quality of life in elderly individuals. Conservative therapy has positive effect on patients whose primary symptom pain. However, severe cases with presence hypesthesia and paresis, surgical treatment comes into consideration. The aim surgery to decompress neurovascular elements compressed by narrowed canal while preserving stability. Conventional laminectomy, or without fusion, been considered effective for this...
Dynamic stabilization devices were developed to reduce spinal hypermobility while preserving a certain degree of physiological motion. Our goal was assess radiographic and clinical outcomes patients treated with surgical decompression the LimiflexTM implant. We investigated effect LimiFlex implantation on post-operative translation angulation in 36 patients stenosis degenerative spondylolisthesis Meyerding Grade I decompression dynamic stabilization. Significant improvements...