A5071050099- Prion Diseases and Protein Misfolding
- Trace Elements in Health
- Neurological diseases and metabolism
- Metallurgy and Material Science
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- RNA regulation and disease
- Advanced Photocatalysis Techniques
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Amino Acid Enzymes and Metabolism
- Amyotrophic Lateral Sclerosis Research
- Alcoholism and Thiamine Deficiency
- TiO2 Photocatalysis and Solar Cells
- Microbial metabolism and enzyme function
- Animal Genetics and Reproduction
- Food Allergy and Anaphylaxis Research
- Monoclonal and Polyclonal Antibodies Research
- Polyamine Metabolism and Applications
- Porphyrin Metabolism and Disorders
- Healthcare and Environmental Waste Management
- Biochemical effects in animals
- Child Nutrition and Feeding Issues
- Folate and B Vitamins Research
- Bacteriophages and microbial interactions
- Food Industry and Aquatic Biology
Aristotle University of Thessaloniki
2014-2024
Friedrich-Loeffler-Institut
2017
Roslin Institute
2017
University of Edinburgh
2017
Interaction Hôtes Agents Pathogènes
2017
Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta
2017
École Nationale Vétérinaire de Toulouse
2017
MRC Prion Unit
2013-2017
Laboratoire de Chimie
2012-2013
Centre for Research and Technology Hellas
2003-2013
YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role not completely understood but elevated in the brain cerebrospinal fluid (CSF) several neurological neurodegenerative diseases associated with inflammatory processes. Yet precise characterization of dementia cases missing. In present study, we comparatively analysed levels CSF samples from dementias different aetiologies characterized presence cortical pathology...
To analyze the diagnostic sensitivity and specificity of various brain-derived proteins (14-3-3, Tau, neuron specific enolase [NSE], S100b) in CSF patients with Creutzfeldt-Jakob disease (CJD) to biologic factors that modify these parameters.CSF was tested for 14-3-3, NSE, S100b 1,859 sporadic, genetic, iatrogenic, variant CJD, 1,117 controls.The highest achieved 14-3-3 Tau sporadic CJD (85% 86%), a combined determination S100b, or NSE increased over 93%. A multivariate analysis showed all...
To date, cerebrospinal fluid analysis, particularly protein 14-3-3 testing, presents an important approach in the identification of Creutzfeldt–Jakob disease cases. However, one special point criticism testing is specificity differential diagnosis rapid dementia. The constant observation increased referrals national surveillance centres over last years raises concern declining due to higher number tests performed various neurological conditions. Within framework a European Community...
A total of 51 goats, including seven clinical cases, from the first herd in Greece reported to have scrapie was examined discern an association between susceptibility and polymorphisms gene encoding prion protein (PrP). Each animal evaluated for signs disease, histopathological lesions associated with scrapie, presence detectable protease-resistant PrP brain genotype. Eleven different genotypes at least five unique predicted mature amino acid sequences were found. These included codons 143...
Abstract Introduction Neurofilament light (NFL) levels in the cerebrospinal fluid are increased several neurodegenerative dementias. However, their diagnostic accuracy differential context is unknown. Methods Cerebrospinal NFL were quantified nonprimarily neurological and psychiatric diseases (n = 122), mild cognitive impairment 48), Alzheimer's disease 108), dementia with Lewy bodies/Parkinson's 53), vascular 46), frontotemporal 41), sporadic Creutzfeldt‐Jakob (sCJD, n 132), genetic prion...
Detection of SARS-CoV-2 in sewage has been employed by several researchers as an alternative early warning indicator virus spreading communities, covering both symptomatic and asymptomatic cases. A factor that can seriously mislead the quantitative measurement viral copies is adsorption fragments onto highly porous solids suspended wastewater, making them inaccessible. This depends not only on available amount solids, but also other dissolved chemicals which may influence capacity...
Prions result from the misfolding and selective accumulation of host-encoded prion protein (PrP) in brain. Despite intensive research on mammalian models, basic questions about biological role PrP evolutionary origin disease remain unanswered. Following our previous identification novel fish homologues, here we generated new sequences performed genomic analysis to demonstrate existence two homologous loci bony fish, which display extensive molecular variation are highly expressed adult...
In this report, we present the first physical characterization of Creutzfeld-Jakob disease agent. Preparations with high yields infectivity (assayed infectious units) were obtained by a novel, gentle procedure in which initially sedimenting Gp34 ("prion" protein) was disaggregated variety criteria no subsequent loss infectivity. Studies preparation indicate that most agent has both viruslike size and density. velocity sedimentation isopycnic sucrose gradients, comigrated nucleic acid-protein...
The analysis of markers in the cerebrospinal fluid (CSF) is useful diagnosis sporadic Creutzfeldt-Jakob disease (sCJD). However, time at which study these most sensitive remains controversal. To assess influence sampling on value CSF tests sCJD. In framework a multinational European study, we studied results 14-3-3, S100b, neurone specific enolase (NSE) and tau protein 833 samples from sCJD patients different stages 66 sequentially repeated lumbar punctures (LP). 14-3-3 tended to increase...
The 14-3-3 protein test has been shown to support the clinical diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) when associated with an adequate context, and a high differential potential for CJD attributed other cerebrospinal fluid (CSF) proteins such as tau protein, S100b neuron specific enolase (NSE). So far there only limited information available about biochemical markers in genetic transmissible spongiform encephalopathies (gTSE), although they represent 10-15% human TSEs. In this...
Abstract Introduction Accurate diagnosis of prion diseases and discrimination from alternative dementias gain importance in the clinical routine, but partial overlap cerebrospinal fluid (CSF) biomarkers impedes absolute differential diagnostic context. Methods We established parameters for disease quantification CSF α‐synuclein patients with sporadic ( n = 234) genetic 56) diseases, cases cognitive impairment/dementia or neurodegenerative 278), neurologic control group 111). Results An...
Amyotrophic lateral sclerosis (ALS) is a rare progressive neurodegenerative disease that affects upper and lower motor neurons. As the molecular basis of still elusive, development high-throughput sequencing technologies, combined with data mining techniques machine learning methods, could provide remarkable results in identifying pathogenetic mechanisms. High dimensionality major problem when applying biomedical analysis, since huge number features available for limited samples. The aim...
A total of 216 local crossbred sheep from 16 scrapie-affected Greek flocks and 210 purebred the milk breeds Chios Karagouniko healthy were analysed for scrapie-linked polymorphisms in prion protein (PrP) gene. Of this case-control study, 96 clinical cases, 25 subclinical cases (asymptomatic at moment euthanasia but positive by histopathology and/or ELISA detecting proteinase-resistant PrP) 95 controls (negative all evaluations). Polymorphisms codons 136, 154 171 determined denaturing...
Several models have been proposed for the infectious agents that cause human Creutzfeldt-Jakob disease (CJD) and sheep scrapie. Purified proteins extracted nucleic acids are not infectious. To further identify critical molecular components of CJD agent, 120S material with reduced prion protein (PrP) was treated guanidine hydrochloride or SDS. Particulate soluble were then separated by centrifugation molecularly characterized. Conditions optimally solubilized residual PrP and/or acid-protein...
Gliosis (hyperplasia and hypertrophy of astrocytes), the fundamental response central nervous system to tissue destruction, typically becomes apparent only several weeks after injury. The biochemical hallmark this is a marked accumulation specific astrocyte intermediate filament glial fibrillary acidic protein (GFAP). To date despite its importance, mechanisms GFAP gene regulation have not been studied in any developmental or pathological our knowledge, molecular signals for mRNA are...
Patients with suspected Creutzfeldt-Jakob disease (CJD) often have routine cerebrospinal fluid (CSF) analysis performed to exclude treatable inflammatory conditions; however, little information is available about the range of results obtained for CSF tests in patients sporadic CJD and other transmissible spongiform encephalopathies (TSE). Data from 450 47 TSEs were collected as part an EC-supported multinational study. Raised white cell counts >5 cells/microl found three 298 CJD, two 7 one...
Prion diseases are transmissible encephalopathies associated with the conversion of physiological form prion protein (PrPC) to disease-associated (PrPSc). Despite intense research, no therapeutic or prophylactic agent is available. The catechol-type diterpene Carnosic acid (CA) and its metabolite Carnosol (CS) from Rosmarinus officinalis have well-documented anti-oxidative neuroprotective effects. Since oxidative stress plays an important role in pathogenesis diseases, we investigated...
Prions are postulated to be the infectious agents of a family transmissible, fatal, neurodegenerative disorders affecting both humans and animals. The possibility prion transmission constitutes public-health risk that confronts regulatory authorities everywhere. main problem in handling prions is fact they extremely resistant standard decontamination methods. Thus, use harsh expensive practices destroy inevitable. development applicable efficient prion-inactivation still highly important for...