A5087609865- Prion Diseases and Protein Misfolding
- RNA Research and Splicing
- Trace Elements in Health
- Neurological diseases and metabolism
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Healthcare and Environmental Waste Management
- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- HIV Research and Treatment
- Metallurgy and Material Science
- Biochemical effects in animals
- Advanced Photocatalysis Techniques
- TiO2 Photocatalysis and Solar Cells
- Studies on Chitinases and Chitosanases
- Immune cells in cancer
- Olfactory and Sensory Function Studies
- Nuclear Receptors and Signaling
- Folate and B Vitamins Research
- Advanced biosensing and bioanalysis techniques
- Microbial Fuel Cells and Bioremediation
- Cancer-related molecular mechanisms research
- Entomopathogenic Microorganisms in Pest Control
- Parasites and Host Interactions
- RNA modifications and cancer
Aristotle University of Thessaloniki
2012-2024
MRC Prion Unit
2017
Centre for Research and Technology Hellas
2009
YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role not completely understood but elevated in the brain cerebrospinal fluid (CSF) several neurological neurodegenerative diseases associated with inflammatory processes. Yet precise characterization of dementia cases missing. In present study, we comparatively analysed levels CSF samples from dementias different aetiologies characterized presence cortical pathology...
Abstract Introduction Neurofilament light (NFL) levels in the cerebrospinal fluid are increased several neurodegenerative dementias. However, their diagnostic accuracy differential context is unknown. Methods Cerebrospinal NFL were quantified nonprimarily neurological and psychiatric diseases (n = 122), mild cognitive impairment 48), Alzheimer's disease 108), dementia with Lewy bodies/Parkinson's 53), vascular 46), frontotemporal 41), sporadic Creutzfeldt‐Jakob (sCJD, n 132), genetic prion...
Detection of SARS-CoV-2 in sewage has been employed by several researchers as an alternative early warning indicator virus spreading communities, covering both symptomatic and asymptomatic cases. A factor that can seriously mislead the quantitative measurement viral copies is adsorption fragments onto highly porous solids suspended wastewater, making them inaccessible. This depends not only on available amount solids, but also other dissolved chemicals which may influence capacity...
Abstract Introduction Accurate diagnosis of prion diseases and discrimination from alternative dementias gain importance in the clinical routine, but partial overlap cerebrospinal fluid (CSF) biomarkers impedes absolute differential diagnostic context. Methods We established parameters for disease quantification CSF α‐synuclein patients with sporadic ( n = 234) genetic 56) diseases, cases cognitive impairment/dementia or neurodegenerative 278), neurologic control group 111). Results An...
Amyotrophic lateral sclerosis (ALS) is a rare progressive neurodegenerative disease that affects upper and lower motor neurons. As the molecular basis of still elusive, development high-throughput sequencing technologies, combined with data mining techniques machine learning methods, could provide remarkable results in identifying pathogenetic mechanisms. High dimensionality major problem when applying biomedical analysis, since huge number features available for limited samples. The aim...
Prion diseases are transmissible encephalopathies associated with the conversion of physiological form prion protein (PrPC) to disease-associated (PrPSc). Despite intense research, no therapeutic or prophylactic agent is available. The catechol-type diterpene Carnosic acid (CA) and its metabolite Carnosol (CS) from Rosmarinus officinalis have well-documented anti-oxidative neuroprotective effects. Since oxidative stress plays an important role in pathogenesis diseases, we investigated...
Prion diseases, also known as Transmissible Spongiform Encephalopathies (TSEs), are protein-based neurodegenerative disorders (NDs) affecting humans and animals. They characterized by the conformational conversion of normal cellular prion protein, PrPC, into pathogenic isoform, PrPSc. diseases invariably fatal despite ongoing research, no effective prophylactic or therapeutic avenues currently available. Anthocyanins (ACNs) unique flavonoid compounds interest in their use potential...
Background: Accurate diagnosis of Alzheimer’s disease (AD) and frontotemporal dementia (FTD) represents a health issue due to the absence traits. We assessed performance SIMOA panel in cerebrospinal fluid (CSF) from 43 AD 33 FTD patients with 60 matching Control subjects combination demographic–clinical characteristics. Methods: 136 (AD: n = 43, FTD: 33, Controls: 60) participated. Single-molecule array (SIMOA), glial fibrillary acidic protein (GFAP), neurofilament light (NfL), TAU,...
Abstract Background Amyotrophic Lateral Sclerosis (ALS) is a highly heterogenous neurodegenerative disorder that primarily affects upper and lower motor neurons, affecting additional cell types brain regions. Underlying molecular mechanisms are still elusive, in part due to disease heterogeneity. Molecular subtyping through integrative analyses including RNA editing profiling novel approach for identification of networks involved pathogenesis. Methods We aimed highlight the role ALS,...
Increasing evidence indicates that microRNAs (miRNAs) are contributing factors to neurodegeneration. Alterations in miRNA signatures have been reported several neurodegenerative dementias, but data prion diseases restricted ex vivo and animal models. The present study identified significant expression pattern alterations the frontal cortex cerebellum of sporadic Creutzfeldt-Jakob disease (sCJD) patients. These changes display a highly regional subtype-dependent regulation correlates with...
Prion diseases are fatal neurodegenerative disorders caused by misfolding of the normal prion protein into an infectious cellular pathogen. Clinically characterized rapidly progressive dementia and accounting for 85% human disease cases, sporadic Creutzfeldt-Jakob (sCJD) is prevalent disease. Although sCJD neuropathological hallmarks well-known, associated molecular alterations elusive due to rapid progression absence preclinical stages. To investigate transcriptome during progression, we...
Prion diseases are transmissible progressive neurodegenerative conditions characterized by rapid neuronal loss accompanied a heterogeneous neuropathology, including spongiform degeneration, gliosis and protein aggregation. The pathogenic mechanisms the origins of prion remain unclear on molecular level. Even though diseases, represent distinct entities, their pathogenesis shares number features disturbed homeostasis, an overload clearance pathways, aggregation pathological altered proteins,...
The present study investigates the potential use of scrapie-protective Q211 S146 and K222 caprine PRNP alleles as targets for selective breeding in Greek goats. Genotyping data from a high number healthy goats with special emphasis on bucks, revealed frequencies these alleles, while estimated probabilities disease occurrence animals carrying were low, suggesting that they can be used selection. represent one largest populations Europe. Thus, considerations presented here are an example...
Photocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton's reagent TiO2, respectively. A comprehensive study photocatalysis on a range microorganisms will significantly establish most efficient method. Here, we report comparative TiO2- Fe+3-based under UV-A diverse microorganisms, including Gram-positive (Staphylococcus aureus) Gram-negative (Escherichia coli) bacteria,...
Abstract Inorganic cells bearing calcium silicate membranes were prepared and resembled closed chemical gardens. It was demonstrated that these inorganic can successfully be loaded with natural products, proteins plasmid DNA, their cargo released in a controlled manner. These the ability of gardens to act as platforms for sustained delivery biomolecules are expected introduce field biosciences.
The key event in prion pathogenesis is the structural conversion of normal cellular protein, PrPC, into an aberrant and partially proteinase K resistant isoform, PrPSc. Since minimum requirement for a disease phenotype expression endogenous PrP host, species carrying orthologue genes, such as fish, could theory support pathogenesis. Our previous work has demonstrated development abnormal protein deposition sea bream brain, following oral challenge fish with natural infectious material. In...
RNA editing is an epitranscriptomic modification, leading to targeted changes in transcripts. It mediated by the action of ADAR (adenosine deaminases acting on double-stranded (ds) and APOBEC (apolipoprotein B mRNA enzyme catalytic polypeptide-like) appears play a major role pathogenesis many diseases. Here, we assessed its experimental autoimmune encephalomyelitis (EAE), widely used non-clinical model inflammatory diseases central nervous system (CNS), which resembles aspects human multiple...
Abstract BACKGROUND Treatment of liquid hazardous medical wastewater produced by healthcare laboratories is a global matter that has been inadequately addressed. In this study, the potential heterogeneous and homogeneous photocatalytic oxidation to detoxify disinfect such effluents was investigated. Experiments were performed using two toxic one simultaneous infectious composition in pilot scale, novel inactivation system. RESULTS Photocatalytic experimental runs with both showed...
Scrapie, the prion disease of sheep and goats, is a devastating malady small ruminants. Due to its infectious nature, epidemic outbreaks may occur in flocks/herds consisting highly susceptible animals. Field studies identified scrapie-protective caprine PrP variants, harboring specific single amino acid changes (Met-142, Arg-143, Asp-146, Ser-146, His-154, Gln-211 Lys-222). Their effects are under further evaluation, aim determine most protective allele. We assessed some these variants...