A5017457151- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Alzheimer's disease research and treatments
- Amino Acid Enzymes and Metabolism
- Aortic Disease and Treatment Approaches
- RNA regulation and disease
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cardiac Valve Diseases and Treatments
- Cardiovascular Effects of Exercise
- Aortic aneurysm repair treatments
- Lipoproteins and Cardiovascular Health
- Vitamin D Research Studies
- Parkinson's Disease Mechanisms and Treatments
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Amyotrophic Lateral Sclerosis Research
- Diet and metabolism studies
- Acute Myocardial Infarction Research
- S100 Proteins and Annexins
- Diabetes Treatment and Management
- Peroxisome Proliferator-Activated Receptors
- Bariatric Surgery and Outcomes
- Diabetes Management and Research
- Cardiovascular Function and Risk Factors
- Dementia and Cognitive Impairment Research
- Alcoholism and Thiamine Deficiency
University Hospital of Zurich
2015-2024
University of Zurich
2017-2024
University of Göttingen
2009-2018
University Hospital of Geneva
2015
ETH Zurich
2015
Institute of Social and Preventive Medicine
2015
University of Lausanne
2015
Swiss Integrative Center for Human Health
2015
Cardiac troponin (cTn) T and cTnI are considered cardiac specific equivalent in the diagnosis of acute myocardial infarction. Previous studies suggested rare skeletal myopathies as a noncardiac source cTnT. We aimed to confirm reliability/cardiac specificity cTnT patients with various muscle disorders (SMDs).We prospectively enrolled presenting muscular complaints (≥2 weeks) for elective evaluation 4 hospitals 2 countries. After workup, were adjudicated into 3 predefined disease categories....
To date, cerebrospinal fluid analysis, particularly protein 14-3-3 testing, presents an important approach in the identification of Creutzfeldt–Jakob disease cases. However, one special point criticism testing is specificity differential diagnosis rapid dementia. The constant observation increased referrals national surveillance centres over last years raises concern declining due to higher number tests performed various neurological conditions. Within framework a European Community...
The 14-3-3 protein test has been shown to support the clinical diagnosis of sporadic Creutzfeldt-Jakob disease (CJD) when associated with an adequate context, and a high differential potential for CJD attributed other cerebrospinal fluid (CSF) proteins such as tau protein, S100b neuron specific enolase (NSE). So far there only limited information available about biochemical markers in genetic transmissible spongiform encephalopathies (gTSE), although they represent 10-15% human TSEs. In this...
The 8-hydroxy-2 deoxyguanosine (8-OHdG) is a product of nucleoside oxidation DNA and reliable marker oxidative stress markers. Increased levels have been reported in the cerebrospinal fluid (CSF) patients with various neurodegenerative disorders.In search biochemical indicator Parkinson's disease (PD), we analyzed 8-OHdG CSF 99 patients, using ELISA to assess differences between disorders.Statistically significant higher (p = 0.022) non-demented PD as compared control group were observed. No...
Objectives. To identify proteins differentially expressed in schizophrenia patients, we collected 50 µl cerebrospinal fluid from 17 first-episode patients and 10 healthy controls. Methods. Their were separated by two-dimensional gel electrophoresis without using any depletion method identified mass spectrometry. Results. Approximately 550 spots detected, six of which had significantly different intensities compared to control specimens. We able validate individual samples the upregulation...
Objective— Blood-borne biomarkers reflecting atherosclerotic plaque burden have great potential to improve clinical management of coronary artery disease and acute syndrome (ACS). Approach Results— Using data integration from gene expression profiling thrombi versus peripheral blood mononuclear cells proteomic analysis plaque–derived secretomes healthy tissue secretomes, we identified fatty acid–binding protein 4 (FABP4) as a biomarker candidate for disease. Its diagnostic prognostic...
Acute aortic dissection (AAD) is a rare condition, but together with acute myocardial infarction (AMI) and pulmonary embolism (PE) it belongs to the most relevant life-threatening causes of chest pain. Until now, there has been no specific blood test in diagnostic workup AAD. To identify clinically biomarkers for AAD, we applied Proseek® Multiplex assays plasma samples from patients AMI, PE, thoracic aneurysm (TAA), non-cardiovascular pain (nonCVD). Subsequently, validated top hits using...
Abstract Aims/hypothesis Post-bariatric hypoglycaemia is an increasingly recognised complication of bariatric surgery, manifesting particularly after Roux-en-Y gastric bypass. While hyperinsulinaemia established pathophysiological feature, the role counter-regulation remains unclear. We aimed to assess counter-regulatory hormones and glucose fluxes during insulin-induced postprandial in patients with post-bariatric bypass vs surgical non-surgical control individuals. Methods In this...
Abstract A 54‐year old patient with metastatic melanoma presented asymptomatic myositis and myocarditis after combined immune checkpoint inhibitors (ICI) therapy (anti‐programmed cell death receptor‐1, anti‐lymphocyte activating gene‐3, anti‐indoleamine 2,3‐dioxygenase‐1). The diagnosis was based on the typical time window ICI, recurrence upon re‐challenge, elevations of CK, high‐sensitive troponin T (hs‐TnT) I (hs‐TnI), mild NT‐proBNP increase, positive magnetic resonance imaging criteria....
179795 -Boosting sleep to excel during the day: Elucidating influence of network dynamics on brain and
Aims: To investigate the effect of empagliflozin on glucose dynamics in individuals suffering from postbariatric hypoglycemia (PBH) after Roux-en-Y gastric bypass (RYGB). Methods: Twenty-two adults with PBH RYGB were randomized to 25 mg or placebo once daily over 20 days a randomized, double-blind, placebo-controlled, crossover trial. The primary efficacy outcome was amplitude plasma excursion (peak nadir) during mixed-meal tolerance test (MMTT). Outcomes outpatient period assessed using...
Background and purpose Cerebrospinal fluid (CSF) analysis supports the clinical diagnosis of sporadic Creutzfeldt−Jakob disease (sCJD) when applied within an adequate context. A diagnostic potential has been attributed to CSF proteins such as 14‐3‐3, but also tau protein, phosphorylated (181P) (p‐tau) amyloid β 1–42 , S100B neuron‐specific enolase (NSE). There only limited information available about contribution in differentiation various molecular sCJD subtypes. Methods The levels...
Abstract The cellular prion protein (PrP c ) is a multifunctional, highly conserved and ubiquitously expressed protein. It undergoes number of modifications during its post‐translational processing, resulting in different PrP glycoforms truncated fragments. Limited data are available humans on the expression cleavage . In this study we investigated isoform composition cerebrospinal fluid from patients with human diseases. first group was affected by sporadic Creutzfeldt–Jakob disease...
Cerebrospinal fluid (CSF) contains a dynamic and complex mixture of proteins, which can reflect physiological pathological state the central nervous system. In our present study, we show CSF protein patterns from patients with two most frequent subtypes sporadic Creutzfeldt−Jakob disease (sCJD) defined by codon 129 genotype (MM, MV, VV) protease-resistant form prion (type 1 type 2). The densitometric analysis 2D gels showed up-regulation 27 down-regulation 3 proteins in MM-sCJD as well 24...
Abstract Background: Point of care (POC) assays for cardiac troponins I or T (cTnI cTnT) may accelerate the diagnosis patients with suspected acute coronary syndrome (ACS). However, their clinical utility according to 0 h/3 h algorithm recommended by European Society Cardiology (ESC) non-ST elevation myocardial infarction (NSTEMI) is unknown. Methods: Blood samples from 90 ACS were obtained at hospital admission and 3 later. Concentrations cTn determined using five POC (AQT90 FLEX cTnI cTnT;...
To date, no plaque-derived blood biomarker is available to allow diagnosis, prognosis or monitoring of atherosclerotic vascular diseases. In this study, specimens thrombendarterectomy material from carotid and iliac arteries were incubated in protein-free medium obtain plaque control secretomes for subsequent subtractive phage display. The selection nine secretome-specific antibodies the analysis their immunopurified antigens by mass spectrometry led identification 22 proteins. One them,...
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited heart muscle disease characterized by fibrofatty replacement of the myocardium and arrhythmias. Biventricular involvement in ARVC may lead to failure. This study aimed investigate role plasma biomarkers soluble (s)ST2, Galectin-3 (Gal-3) GDF-15 predicting biventricular adverse outcomes ARVC.ARVC patients from 2 independent cohorts, were studied. The Bejing (Chinese) cohort (n = 108) was discovery cohort, whereas Zurich...
Sporadic Creutzfeldt–Jakob disease (sCJD) is characterized by wide clinical and pathological variability, which mainly influenced the conformation of misfolded prion protein, methionine valine polymorphism at codon 129 protein gene. This heterogeneity likely implies differences in molecular cascade that leads to development certain phenotypes. In this study, we investigated proteome frontal cortex patients with two most common sCJD subtypes (MM1 VV2) using 2D-DIGE MS. Analysis 2D maps...