Malachite green (MG) is a synthetic dye that uses ranges from its application as tissue to an antiparasitic in aquaculture. Several studies have reported the presence of this compound food dyes and meat fish raised captivity for human consumption, suggesting risks both end user those who handle these products because MG toxic properties described literature. Here we evaluated cytotoxic genotoxic profiles four different cell lines (ACP02, L929, MNP01, MRC-5). Two are stomach cells (normal...

In this study we examined the karyotypes of morphologically indistinguishable populations electric knifefish Gymnotus carapo sensu stricto from Eastern Amazon Brazil. These were identified unambiguously on basis external morphology, meristics, and pigmentation.Specimens one five localities exhibited a karyotype previously not documented for species in basin: 2n = 40 (34M/SM+6ST/A). Samples other four different karyotype: 42 (30M/SM+12ST/A), which had described. Specimens all presented...

Gymnotus (Gymnotidae, Gymnotiformes) is the Neotropical electric fish genus with largest geographic distribution and number of species, 33 which have been validated. The diploid varies from 2n = 39-40 to 54. Recently we studied karyotype morphologically indistinguishable samples five populations G. carapo sensu stricto Eastern Amazon Brazil. We found two cytotypes, 42 (30 M/SM + 12 ST/A) 40 (34 6 concluded that differences between cryptic species are due pericentric inversions one tandem...

In this paper we describe the karyotypes of two sympatric species Gymnotus from Marajó Island, Pará, in Eastern Amazon basin Brazil. One these species, G. mamiraua, has not previously been identified area. Karyotype studies demonstrate that cf. carapo Linnaeus, 2n=42 (30M/SM+12ST/A) and mamiraua Albert & Crampton, 2n=54 (50M/SM+4ST). The Nucleolar Organizer Region (NOR) was found distal region short arm pair 20 interstitial 1 mamiraua. Both have centromeric constitutive heterochromatin...

We analyzed the karyotypes of three specimens fishes genus Hypostomus Lacepede, 1803 (Loricaridae) from Xingu River (Amazon region). used conventional staining techniques, including C-banding, Ag-NOR staining, CMA3- and DAPI-banding. Each specimen had a different cytotype: sp. Xingu-1 2n=64 (32M/SM, 32ST/A); Xingu-2 has 2n=66 34ST/A), Xingu-3 2n=65 (38M/SM, 26ST/A + 1 B). The cytotypes showed similar C-, DAPI-banding patterns. nucleolus organizing regions were located in short arm chromosome...

We studied the karyotypes of Hassar cf. orestis and an undescribed species from Jarí River Opsodoras ternetzi, H. Platydoras costatus Xingú River, all with 2n = 58. Constitutive heterochromatin is located in centromere most metacentric pairs; some chromosomes this banding not present, or it on whole chromosome arm distal regions. The NOR a single biarmed pair at region short orestis, P. long O. ternetzi proximal species. In (except for sp.) CMA3 analysis revealed rich G-C coincident NOR....

AbstractThis is the description of a method to obtain DAPI-banding in fishes' chromosomes by its denaturation using formamide. The slides are aged an incubator and dehydrated ethanol series. next step formamide 70% staining with DAPI. pattern obtained mostly C-band like, but matches only partially usually found Barium Hydroxide technique. bands also do not match band CMA3. These data review literature bring conclusion that banding related DNA base composition instead being chromosome...

The Ancistrus genus has extensive chromosomal diversity among species, including heteromorphic sex chromosomes occurrence. However, studies have been shown that may still be underestimated. Repetitive sequences represent a large part of eukaryotic genomes, associated with mechanisms karyotypic diversification, evolution. This study analyzed the karyotype diversification two species (Ancistrus sp. 1 and 2) from Amazon region by classical molecular markers. Conventional chromosome bands...

Peckoltia is widely distributed genus in the Amazon and Orinoco basins Guiana Shield, containing 18 valid species, distinct morphotypes still needing description scientific literature due to its great taxonomic complexity. This study performed a comparative chromosomal analysis of two undescribed species ( sp. 3 Jarumã 4 Caripetuba) from Brazilian using conventional chromosome bands methods situ localization repetitive DNA 5S 18S rRNA U1 snRNA genes telomeric sequences). Both presented 2n =...

This study describes the karyotypes of three species genus Peckoltia (Loricariidae: Ancistrini). Fishes were collected in Jari (Peckoltia sp. 1 and 2) Xingu rivers vittata (Steindachner, 1881)) Amazon rainforest. Karyotypes 2n = 52 for (FN=102: 16 metacentrics (m), 20 submetacentrics (sm), 14 subtelocentrics (st), 2 acrocentrics (a)) 32 m + sm, 18st, 2a). 44 6st, 2a, 1B) had 53 due to a B chromosome. The differ chromosomal morphology. Nucleolar Organizer Regions (NOR) was identified within...

Abstract Three specimes of Baryancistrus aff. niveatus were studied cytogenetically by conventional staining, C banding, Ag-NORs, CMA3, DAPI and FISH with human telomeric probes. The results revealed a complement consisting 52 metacentric, submetacentric subtelocentric type chromosomes. banding showed that heterochro-matin is located at pairs 1, 3, 6, 10, 11 22 in different positions. NORs the short arm pair coinciding positive C-band. This region was also marked which heterochromatic blocks...

Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results compared to literature. Mitotic chromosomes obtained kidney cells analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI sequential staining fluorochrome situ hybridization with human telomere probes. All individuals showed a chromosome number 2n = 48 (12 M/SM 36 ST/A) FN 60. No differences detected between male female karyotypes,...

Pseudacanthicus is a genus of Neotropical fish with eight valid species, in addition to numerous lineages not formally identified. It occurs along the Amazon and Tocantins River basins, Suriname Guiana shield. There are no karyotypic data literature for species this genus. Here, karyotypes three (P. spinosus, P. leopardus sp.) were comparatively analyzed by classical cytogenetics fluorescence situ hybridization using 18S 5S rDNA probes, U2 snDNA telomeric sequences. The presented 52...

O presente estudo visa estimar a biomassa dos estoques de peixes na area da represa formada pela barragem rejeitos Mina Sossego em Canaa Carajas, capturados com uso tarrafa para peixes. trabalho prospeccao foi realizado durante o periodo 29 fevereiro 06 marco 2004, 6 meses apos fechamento e imediatamente antes do inicio despejo rejeitos. Os valores medios estimados captura por unidade encoberta (CPUA) foram 8,4 g m - ² 16.922,9 kg, respectivamente. grupos mais representativos foram: piabas,...

The genus Makalata is a taxonomically complex group of rodents on which few cytogenetic studies have been performed. Most the published karyotypes were described based only conventional chromosome staining. Here, we studied from two Brazilian Amazonian states, Amapá and Pará, by Giemsa-staining, G- C-banding, AgNO3-staining FISH with 18S rDNA telomeric sequences probes. We observed 2n = 66/FN 124 in Pará state population sp; 72/FN 128 M. didelphoides. Multiple rearrangements may given rise...

Ancistrus is a highly diverse neotropical fish genus that exhibits extensive chromosomal variability, encompassing karyotypic morphology, diploid chromosome number (2n = 34-54), and the evolution of various types sex systems. Robertsonian rearrangements related to unstable sites are here described. Here, karyotypes two species were comparatively analyzed using classical cytogenetic techniques, in addition isolation, cloning, sequencing, molecular characterization, fluorescence situ...

Abstract Archolaemus , one of the five genera Neotropical freshwater fish family Sternopygidae (Gymnotiformes), was long considered a monotypic genus represented by blax . Currently, it consists six species, most them occurring in Amazon region. There are no cytogenetic data for species this genus. In present study, we used classical cytogenetics (conventional staining and C‐banding) molecular (probes telomeric sequences multigenic families 18S rDNA, 5S U2 snDNA) to study karyotype janeae...

This study described the karyotype of Geophagus cf. proximus. Specimens were collected in Água Preta Lake, Parque Ambiental de Belém, Pará State, Brazil. The 2n = 48 chromosomes (FN 60: 12M/SM+36ST/A) and no sexual chromosome differentiation. C-banding showed centromeric staining all chromosomes. first pair, besides coloration, presented a totally heterochromatic long arm. nucleolus organizer regions (NORs) studied by means AgNO3. NORs located at short arm second pair.

O objetivo deste trabalho foi realizar um levantamento acerca das concepções dos moradores do município de Abaetetuba no estado Pará, sobre uso e consumo quelônios da região. A área estudo abrangeu 38 bairros 72 ilhas, que constituem a chamada região ilhas Município Norte Brasil. Para aquisição dados foram aplicados formulários online mês outubro 2020 posteriormente os processados através programa Microsoft Office Excel (Versão 2016). Foram obtidas 146 respostas abrangeram 14 9 comunidades,...

Loricariidae é uma das mais especiosas famílias dentro da ordem Siluriformes, possuindo cerca de 800 espécies válidas. O gênero Peckoltia (Loricariidae, Hypostominae), atualmente contém 19 válidas, apresentando status taxonômico confuso. objetivo deste trabalho foi analisar comparativamente os cariótipos cf. braueri e sp., coletadas no município Abaetetuba – PA por métodos clássicos (coloração convencional bandeamento C) Hibridização in situ Fluorescente com sondas rDNA 18S, 5S telomérica....

The present work aimed, through the method of bibliographic review, to show that conventional and molecular cytogenetics is one tools for taxonomic evolutionary studies neotropical fishes.Based on methodology used, which was divided into two stages, first being booking data collect second analysis discussion results, we got three used in fishes whose results (indications chromosome dynamics may be involved evolution speciation) contributed what intended this work: assertion methods study...