A5016008254- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Interference and Gene Delivery
- Nerve injury and regeneration
- Virus-based gene therapy research
- S100 Proteins and Annexins
- Traumatic Brain Injury and Neurovascular Disturbances
- Force Microscopy Techniques and Applications
- Neurological Complications and Syndromes
- Epilepsy research and treatment
- Advanced Electron Microscopy Techniques and Applications
- Extracellular vesicles in disease
- Signaling Pathways in Disease
- Pain Mechanisms and Treatments
- Immune cells in cancer
- Adenosine and Purinergic Signaling
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
Yonsei University
2023
Institute for Basic Science
2023
Clemson University
2016-2022
Abstract Excessive and prolonged neuroinflammation leads to neuronal cell death limits functional recovery after traumatic brain injury (TBI). Dexamethasone (DX) is a steroidal anti-inflammatory agent that known attenuate early expression of pro-inflammatory cytokines associated with activated microglia/macrophages. In this study, we investigated the effect dexamethasone-conjugated hyaluronic acid (HA-DXM) incorporated in hydrolytically degradable, photo-cross-linkable poly (ethylene)...
Among the complex pathophysiological events following spinal cord injury (SCI), one of most important molecular level consequences is a dramatic reduction in neuronal cyclic adenosine monophosphate (cAMP) levels. Many studies shown that rolipram (Rm), phosphodiesterase IV inhibitor, can protect against secondary cell death, reduce inflammatory cytokine levels and immune infiltration, increase white matter sparing functional improvement. Previously, we developed polymeric micelle...
Functional recovery following traumatic brain injury (TBI) is limited due to progressive neuronal damage resulting from secondary injury-associated neuroinflammation. Steroidal anti-inflammatory drugs, such as dexamethasone (DX), can reduce neuroinflammation by activated microglia and infiltrated macrophages. In our previous work, we developed hydrolytically degradable poly(ethylene) glycol-bis-(acryloyloxy acetate) (PEG-bis-AA) hydrogels with (DX)-conjugated hyaluronic acid (HA-DXM)...
Abstract Multiple age-related and injury-induced characteristics of the adult central nervous system (CNS) pose barriers to axonal regeneration functional recovery following injury. In situ gene therapy is a promising approach address limited availability growth-promoting biomolecules at CNS injury sites. The ultimate goal our work develop, cationic amphiphilic copolymer for simultaneous delivery drug therapeutic nucleic acids promote plasticity after spinal cord Previously, we reported...
To understand how the molecular machinery of synapses works, it is essential to determine an inventory synaptic proteins at a subsynaptic resolution. Nevertheless, are difficult localize because low expression levels and limited access immunostaining epitopes. Here, we report on exTEM (epitope-exposed by expansion-transmission electron microscopy) method that enables imaging in situ. This combines TEM with nanoscale resolution expandable tissue-hydrogel hybrids for enhanced immunolabeling...
Neuronal axons damaged by traumatic injury are unable to spontaneously regenerate in the mammalian adult central nervous system (CNS), causing permanent motor, sensory, and cognitive deficits. Regenerative failure CNS results from a complex pathology presenting multiple barriers, both presence of growth inhibitors extrinsic microenvironment intrinsic deficiencies neuronal biochemistry, axonal regeneration functional recovery. There many strategies for after including antagonism...
Aim: To develop poly(lactide-co-glycolide)-graft-polyethylenimine (PgP) as a nanocarrier for the delivery of rolipram (Rm) and evaluate therapeutic efficacy Rm-loaded PgP (Rm-PgP) on secondary injury motor function in rat traumatic brain (TBI) model. Materials & methods: Rm-PgP was injected injured lesion immediately after TBI using microinjection pump. Secondary pathologies such inflammatory response, apoptosis astrogliosis were assessed by histological analysis functional recovery assorted...