Although biodegradable polymer/ceramic composite scaffolds can overcome the limitations of conventional ceramic bone substitutes, osteogenic potential these needs to be further enhanced for efficient tissue engineering. In this study, bonelike apatite was efficiently coated onto scaffold surface by using instead polymer and an accelerated biomimetic process enhance scaffold. The creation bonelike, apatite-coated achieved incubating in simulated body fluid (SBF). growth on porous...

Dental implant surgeries involve the insertion of fixtures into alveolar bones to replace missing teeth. When availability bone at surgical site is insufficient, graft particles are filled in for successful reconstruction. Bone induce regeneration over several months site. Subsequently, can be inserted recipient Thus, conventional dental surgery performed steps, which turn increases treatment period and cost involved. Therefore, reduce time minimize costs, a novel hybrid scaffold with that...

Magnetic microrobots can be miniaturized to a nanometric scale owing their wireless actuation, thereby rendering them ideal for numerous biomedical applications. As result, nowadays, there exist several mechano-electromagnetic systems actuation. However, magnetic actuation is not sufficient implementation in applications, and further functionalities such as imaging heating are required. This study proposes multimodal electromagnetic system comprised of three pairs Helmholtz coils, pair...

Glaucoma treatment involves reducing the intraocular pressure (IOP), which can damage optic nerve, to a normal range. Aqueous drainage devices may be used for treatment, and variety of have been proposed. However, they non-variable uniform inner diameter, makes it difficult accommodate IOP fluctuations that occur after glaucoma surgery. To ensure effective in early post-operative period with low late high IOP, diameter should expandable over time allow smooth aqueous drainage. Here, we...

Abstract Background Fabry’s cardiomyopathy patients receiving enzyme replacement therapy (ERT) have no validated cardiac functional biomarkers to evaluate the effect of ERT. Purpose We aimed investigate assess response ERT on heart and predict clinical outcomes. Methods Patients with disease having LV wall thickness ≥12mm were prospectively enrolled treated Agalsidase beta. The primary endpoint was change exercise peak E/e’ by diastolic stress echocardiography (DSE) during ERT; secondary...

Abstract Background Heart failure with preserved ejection fraction (HFpEF) is a clinical syndrome caused by various pathophysiologic mechanisms, and has recently received increasing attention. Many research results show that the longitudinal left ventricular (LV) function deteriorated, however, in real world, we often encounter patients LV global strain (GLS). This study sought to determine prevalence, characteristics, outcome of GLS real-world registry HFpEF. Method The data included 1,798...

Abstract Bone morphogenetic proteins (BMPs) are the most potent osteoinductive growth factors. However, a delivery system is essential to take advantage of effect BMPs. In present study, we tested suitability apatite‐coated poly( D,L ‐lactide‐ co ‐glycolide)/nanohydroxyapatite (PLGA/HA) particulates as carriers for controlled release BMP‐2. The BMP‐2 from PLGA/HA was sustained at least 4 weeks in vitro . A suspended fibrin gel further slowed rate. vivo implantation either Fibrin + or showed...

The low survival rate of graft stem cells after transplantation into recipient tissue is a major obstacle for successful cell therapy. After the site spinal cord injury, face not only hypoxia due to oxygen conditions, but also lack nutrients caused by damaged tissues and poor vascular supply. To improve therapeutic grafting injured cord, we examined effects cotransplanting mouse neural (mNSCs) adipose tissue-derived mesenchymal (AT-MSCs) on mNSC viability. viability mNSCs in coculture with...

Abstract Transplanting stem cells differentiated towards a cardiac lineage can regenerate muscle tissues to treat myocardial infarction. In this study, we tested the hypothesis that transforming growth factor‐ β 1 (TGF‐ 1) induces cardiomyogenic differentiation of adipose‐ derived stromal (ADSCs) in vitro . Rat ADSCs were cultured with TGF‐ (10 ng ml −1 ) for 2 weeks without served as control. The mRNA expression cardiac‐specific gene was induced by 1, while control culture did not show...

In this study, we investigated the dual-pore kagome-structure design of a 3D-printed scaffold with enhanced in vitro cell response and compared mechanical properties scaffolds conventional or offset patterns. The compressive modulus proposed was found to resemble that pattern at similar pore sizes despite higher porosity. Furthermore, surpassed patterns porosities owing structural characteristics kagome structure. Regarding response, adhesion, growth, ALP concentration for 14 days superior...

Background. Current therapies for end-stage renal disease have severe limitations. Dialysis is only a temporary treatment and does not restore kidney function. Transplantation limited by donor organ shortage immune-related problems. Here, we show that the transplantation of fetal precursor cells reconstitutes tissues, reduces uremic symptoms, provides life-saving metabolic support in failure animal models. Methods. Kidney was surgically induced resecting kidneys, leaving approximately 1/6...

Among the complex pathophysiological events following spinal cord injury (SCI), one of most important molecular level consequences is a dramatic reduction in neuronal cyclic adenosine monophosphate (cAMP) levels. Many studies shown that rolipram (Rm), phosphodiesterase IV inhibitor, can protect against secondary cell death, reduce inflammatory cytokine levels and immune infiltration, increase white matter sparing functional improvement. Previously, we developed polymeric micelle...

Abstract Multiple age-related and injury-induced characteristics of the adult central nervous system (CNS) pose barriers to axonal regeneration functional recovery following injury. In situ gene therapy is a promising approach address limited availability growth-promoting biomolecules at CNS injury sites. The ultimate goal our work develop, cationic amphiphilic copolymer for simultaneous delivery drug therapeutic nucleic acids promote plasticity after spinal cord Previously, we reported...

Abstract Bioartificial skin sheet grafts have been utilized to treat large burns and chronic ulcers. However, the trypsinization step harvest cultured from culture dishes damages cells by breaking anchoring proteins lowers their uptake ratio after transplantation. In addition, epidermal require a long fabrication period. To overcome these limitations, we biodegradable poly(lactide‐ co ‐glycolide) (PLGA) microspheres as both cell matrix transplantation vehicle of for regeneration in this...

Abstract Gene delivery offers therapeutic promise for the treatment of neurological diseases and spinal cord injury. Several studies have offered viral vectors as vehicles to deliver agents, yet their toxicity immunogenicity, along with cost large-scale formulation, limits clinical use. As such, non-viral are attractive in that they offer improved safety profiles compared viruses. Poly(ethylene imine) (PEI) is one most extensively studied vectors, but its value limited y cytotoxicity....

Abstract Methylprednisolone (MP) is a glucocorticoid that used as an anti‐inflammatory agent to the treat spinal cord injury (SCI). A low molecular weight chitosan was synthesize chitosan‐MP conjugate, which evaluate gene therapy, and anti‐apoptotic effects of MP. The cytotoxicity nanoparticles transfection efficiency plasmid DNA were evaluated by MTT luciferase assays. chitosan‐MP/pDNA complexes injected into injured these using terminal deoxynucleotide transferase dUTP nick end labeling...