A5044638744- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Genomics and Phylogenetic Studies
- Biocrusts and Microbial Ecology
- Genetics, Aging, and Longevity in Model Organisms
- Aquaculture disease management and microbiota
- Toxin Mechanisms and Immunotoxins
- Ion channel regulation and function
- Signaling Pathways in Disease
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Nuclear Structure and Function
- Plant Reproductive Biology
- Asthma and respiratory diseases
- Microbial infections and disease research
- Erythrocyte Function and Pathophysiology
- Photoreceptor and optogenetics research
- Heat shock proteins research
- Plant Stress Responses and Tolerance
Centre for Genomic Regulation
2020-2023
University of Cambridge
2011
The University of Queensland
2010-2011
Harry Perkins Institute of Medical Research
2002
Sir Charles Gairdner Hospital
2002
Queen Elizabeth II Medical Centre
2002
Retromer is a peripheral membrane protein complex that has pleiotropic roles in endosomal trafficking. The core of retromer possesses three subunits, VPS35, VPS29 and VPS26, play different binding to cargo, regulatory proteins stabilization. We have performed an investigation the thermodynamics assembly using isothermal titration calorimetry (ITC) demonstrating VPS35 acts as central subunit which VPS26 bind independently. Furthermore, we confirm conserved PRLYL motif large critical for...
Stromules are highly dynamic stroma-filled tubules that extend from the surface of all plastid types in multi-cellular plants examined to date. The stromule frequency (percentage plastids with stromules) has generally been regarded as characteristic cell and tissue type. However, present study shows various stress treatments, including drought salt stress, able induce formation epidermal cells tobacco hypocotyls root hairs wheat seedlings. Application abscisic acid (ABA) seedlings induced...
VPS29 is a key component of the cargo-binding core complex retromer, protein assembly with diverse roles in transport receptors within endosomal system. has fold related to metal-binding phosphatases and mediates interactions between retromer other regulatory proteins. In this study we examine functional mammalian VPS29, using X-ray crystallography NMR spectroscopy. We find that although can coordinate metal ions Mn2+ Zn2+ both putative active site at locations, affinity for metals low, lack...
Cyclophilin 40 (CyP40) is a tetratricopeptide repeat (TPR)-containing immunophilin and modulator of steroid receptor function through its binding to heat shock protein 90 (Hsp90). Critical this are the carboxyl-terminal MEEVD motif Hsp90 TPR domain CyP40. Two different models CyP40-MEEVD peptide interaction were used as basis for comprehensive mutational analysis Hsp90-interacting Using CyP40 construct template, 24 amino acids from flanking acidic basic domains individually mutated by...
To assist in the advancement of large-scale production safe Mycoplasma vaccines and other Mycoplasma-based therapies, we developed a culture medium free animal serum components for pneumoniae growth. By establishing workflow method to systematically test different compounds concentrations, provide optimized formulations capable supporting serial passaging robust growth reaching 60 70% biomass obtained rich medium. Global transcriptomic proteomic analysis showed minor physiological changes...
Summary The removal of unwanted genetic material is a key aspect in many synthetic biology efforts and often requires preliminary knowledge which genomic regions are dispensable. Typically, these guided by transposon mutagenesis studies, coupled to deepsequencing (TnSeq) identify insertion points gene essentiality. However, epistatic interactions can cause unforeseen changes essentiality after the deletion gene, leading redundancy maps. Here, we present LoxTnSeq, new methodology generate...
The Cre-Lox system is a highly versatile and powerful DNA recombinase mechanism, mainly used in genetic engineering to insert or remove desired sequences. It widely utilized across multiple fields of biology, with applications ranging from plants, mammals, microbes. A key feature this its ability allow recombination between mutant lox sites. Two the most commonly sites are named lox66 lox71, which recombine create functionally inactive double lox72 site. However, large portion published...
ABSTRACT Comparative genomics among bacteria has been used to gain insight into the minimal number of conserved genes in biological pathways. Essentiality studies have provided information regarding which are non-dispensable (essential, E) for cell growth. Here, we integrated gene conservation, essentiality and function, performed 47 diverse bacterial species. We showed there is a modest positive correlation between genome size essential genes. Interestingly, observed clear shift functions...
Abstract The Cre-Lox system is a highly versatile and powerful DNA recombinase mechanism, mainly used in genetic engineering to insert or remove desired sequences. It widely utilised across multiple fields of biology, with applications ranging from plants, mammals, microbes. A key feature this its ability allow recombination between mutant lox sites, traditionally named lox66 lox71, create functionally inactive double lox72 site. However, large portion the published literature has...
Abstract The removal of unwanted genetic material is a key aspect in many synthetic biology efforts, and often requires preliminary knowledge which genomic regions are dispensable. Typically, these efforts guided by transposon mutagenesis studies, coupled to deep-sequencing (TnSeq) identify insertion points gene essentiality. However, epistatic interactions can cause unforeseen changes essentiality after the deletion gene, leading redundancy maps. Here, we present LoxTnSeq, new methodology...