A5028170463- Influenza Virus Research Studies
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- Virology and Viral Diseases
- RNA Interference and Gene Delivery
- Animal Virus Infections Studies
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Radiomics and Machine Learning in Medical Imaging
- Cardiac Fibrosis and Remodeling
- Pneumonia and Respiratory Infections
- Microfluidic and Bio-sensing Technologies
- Advanced Thermodynamics and Statistical Mechanics
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Hormonal Regulation and Hypertension
- Graph theory and applications
- bioluminescence and chemiluminescence research
- Immune Response and Inflammation
- Gene expression and cancer classification
- Escherichia coli research studies
- Bacterial Infections and Vaccines
Cornell University
2016-2020
University of Massachusetts Chan Medical School
2019-2020
The protocol aims to generate coronavirus (CoV) spike (S) fusion protein pseudotyped particles with a murine leukemia virus (MLV) core and luciferase reporter, using simple transfection procedure of the widely available HEK-293T cell line. Once formed released from producer cells, these pseudovirions incorporate reporter gene. Since they only contain heterologous on their surface, behave like native counterparts for entry steps. As such, are excellent surrogates virions studying viral into...
Abstract Recombinant adeno-associated viruses (rAAVs) are currently considered the safest and most reliable gene delivery vehicles for human therapy. Three serotype capsids, AAV1, AAV2, AAV9, have been approved commercial use in patients, but they may not be suitable all therapeutic contexts. Here, we describe a novel capsid identified clinical sample by high-throughput, long-read sequencing. The capsid, which named AAVv66, shares high sequence similarity with AAV2. We demonstrate that...
Emerging technologies use cell plasma membrane vesicles or "blebs" as an intermediate to form molecularly complete, planar surface mimetics that are compatible with a variety of characterization tools and microscopy methods. This approach enables direct incorporation proteins into supported lipid bilayers without using detergents reconstitution preserves native lipids species. Such system can be advantageous in vitro models vivo surfaces for study the roles drug targets delivery,...
With the development of single-particle tracking (SPT) microscopy and host membrane mimics called supported lipid bilayers (SLBs), stochastic virus-membrane binding interactions can be studied in depth while maintaining control over receptor type concentration. However, several experimental design challenges quantitative image analysis limitations prevent widespread use this approach. One main challenge SPT studies is low signal-to-noise ratio videos, which sometimes inevitable due to small...
Abstract Virus pseudotyping is a useful and safe technique for studying entry of emerging strains influenza virus. However, few studies have compared different reassortant combinations in pseudoparticle systems, or kinetics native viruses their pseudotyped analogs. Here, vesicular stomatitis virus (VSV)-based pseudovirions displaying distinct envelope proteins were tested fusion activity. We produced VSV pseudotypes containing the prototypical X-31 (H3) HA, either alone with strain-matched...
The protocol aims to generate coronavirus (CoV) spike (S) fusion protein pseudotyped particles with a murine leukemia virus (MLV) core and luciferase reporter, using simple transfection procedure of the widely available HEK-293T cell line. Once formed released from producer cells, these pseudovirions incorporate reporter gene. Since they only contain heterologous on their surface, behave like native counterparts for entry steps. As such, are excellent surrogates virions studying viral into...