A5090051366- Tuberculosis Research and Epidemiology
- Steroid Chemistry and Biochemistry
- Mycobacterium research and diagnosis
- Immune cells in cancer
- Ovarian cancer diagnosis and treatment
- Escherichia coli research studies
- Antibiotic Resistance in Bacteria
- Immune Response and Inflammation
- Nitric Oxide and Endothelin Effects
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Renal and related cancers
- Pharmacogenetics and Drug Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biochemical Acid Research Studies
- Cholesterol and Lipid Metabolism
- Apelin-related biomedical research
- Mast cells and histamine
- Drug Transport and Resistance Mechanisms
- Neuropeptides and Animal Physiology
- Metabolomics and Mass Spectrometry Studies
Institute for Medical Biology
2016-2025
Polish Academy of Sciences
2016-2025
Institute of Medical Biology
2014-2020
Despite considerable research effort, the molecular mechanisms of Mycobacterium tuberculosis (Mtb) virulence remain unclear. Cholesterol oxidase (ChoD), an extracellular enzyme capable converting cholesterol to its 3-keto-4-ene derivative, cholestenone, has been proposed play a role in Mtb. Here, we verified hypothesis that ChoD is modifying bactericidal and pro-inflammatory activity human macrophages. We also sought determine contribution complement receptor 3 (CR3)- Toll-like 2...
Abstract Background A growing body of evidence suggests that Mycobacterium tuberculosis (Mtb) uses the host’s cholesterol as a source carbon and energy during infection. Strains defective in transport or degradation exhibit attenuated growth activated macrophages diminished infectivity animal models. The aim this study was to evaluate intracellular replication degradation-deficient Mtb mutant human (MØ) vitro assess functional responses mutant-infected MØ. Results H37Rv strain containing an...
The intracellular pathogen Mycobacterium tuberculosis (Mtb) is constantly exposed to a multitude of hostile conditions and confronted by variety potentially DNA-damaging assaults in vivo, primarily from host-generated antimicrobial toxic radicals. Exposure reactive nitrogen species and/or oxygen causes different types DNA damage, including oxidation, depurination, methylation deamination, that can result single- or double-strand breaks (DSBs). These affect the integrity whole genome and,...
It has been increasingly recognized that SNAIL1 and SNAIL2, as major EMT-inducers, might also be involved in drug resistance of cancer cells. We sought to determine a relation between SNAIL1/2, E-cadherin N-cadherin expression, well ovarian cells’ cisplatin EMT markers’ level. Thus, four cell lines, were used: A2780, A2780cis, SK-OV-3 OVCAR-3. assessed the impact ERK1/2, AKT STAT3 proteins (chosen by profiling activity over 40 signaling proteins) on SNAIL1/2 along with levels. showed...
The most important factors involved in tumor metastasis and angiogenesis are metalloproteinases (MMPs), vascular endothelial growth factor, multifunctional transforming factor β1. These responsible for extracellular matrix degradation, induction of permeability, enhancement cells' invasion metastasis. Elevated expression secretion the above-mentioned correlated with higher aggressiveness tumors low patient survival example, patients ovarian cancer. Therefore, regulation expression,...
The diet supplementation with antioxidants-rich products is a way to protect people from free radical-induced diseases. In this study, we compare the anti-inflammatory/antioxidant activity of two compounds available as supplements: alpha lipoic acid (ALA) and ferulic (FA).The radical scavenging capacity ALA FA in cell system was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Anti-inflammatory both determined, vitro, on THP-1 derived macrophages model, resting (not stimulated)...
Abstract Molecular epidemiological studies of Mycobacterium kansasii are hampered by the lack highly-discriminatory genotyping modalities. The purpose this study was to design a new, high-resolution fingerprinting method for M. . Complete genome sequence ATCC 12478 reference strain searched satellite-like repetitive DNA elements comprising tandem repeats. A total 24 variable-number repeat (VNTR) loci were identified with potential discriminatory capacity. Of these, 17 used polymorphism among...
During metastasis, cancer cells undergo phenotype changes in the epithelial-mesenchymal transition (EMT) process. Extracellular vesicles (EVs) released by are mediators of intercellular communication and play a role metastatic Knowledge factors that influence modifications pre-metastatic niche for migrating carcinoma is important prevention metastasis. We focus here on how progression affected EVs from either epithelial-like HT29-cells or early EMT stage triggered Snail transcription factor...
Our knowledge about the mechanisms utilized by Mycobacterium tuberculosis to survive inside macrophages is still incomplete. One of mechanism that protects M. from host's microbicidal products and allows bacteria involves DNA repair systems such as homologous recombination (HR) nonhomologous end-joining (NHEJ) pathways. It accepted any pathway contributes genome maintenance should be considered potentially important virulence factor. In these studies, we investigated reactive oxygen species,...
Ovarian cancer is one of the most frequent and deadly gynaecological cancers, often resistant to platinum-based chemotherapy, current standard care. Halophilic microorganisms have been shown produce a large variety metabolites, some which show toxicity various cell lines. However, none yet be active against ovarian cells. Here, we examined effects metabolites secreted by halophilic archaea Halorhabdus rudnickae Natrinema salaciae on lines, including lines.1H NMR analyses Hrd. Nnm. culture...
This study tested the hypothesis that Mycobacterium tuberculosis (Mtb) uses a cholesterol oxidase enzyme (ChoD) to suppress toll-like receptor type 2- (TLR2-) dependent signalling pathway modulate macrophages’ immune response. We investigated impact of Mtb possessing or lacking ChoD as well TBChoD recombinant protein obtained from on expression and activation two key intracellular proteins involved in TLR2 human macrophages. Finally, involvement TLR2-related an inflammatory/immunosuppressive...
Although mycobacterial glycolipids are among the first-line molecules involved in host–pathogen interactions, their contribution virulence remains incomplete. Mycobacterium marinum is a waterborne pathogen of fish and other ectotherms, closely related to tuberculosis. Since it causes tuberculosis-like systemic infection widely used as model organism for studying pathogenesis It also an occasional opportunistic human pathogen. The M. surface-exposed lipooligosaccharides (LOS) immunogenic that...