The increasing prevalence of monocultures has reduced floral diversity, diminishing pollen diet variety for bees. This study examines the impact monofloral diets (hazel, rapeseed, pine, buckwheat, Phacelia, goldenrod) on antioxidant enzyme activities in fat body from tergite 3, 5, sternite, and hemolymph honey We show that plants such as goldenrod (rich phenolic compounds flavonoids) increases SOD, CAT, GST, GPx compared to control group. Although it is commonly known a monodiet one stress...

A significant number of studies report growing resistance in nematodes thriving both humans and livestock. This study was conducted to evaluate the vitro vivo anthelmintic efficiency Curcubita pepo (C. pepo) L. hot water extract (HWE), cold (CWE) or ethanol (ETE) on two model nematodes: Caenorhabditis elegans elegans) Heligmosoides bakeri (H. bakeri). Methods: Raman, IR LC-MS spectroscopy analyses were performed studied plant material deliver qualitative quantitative data composition...

When studying honey bee nutrition, it is important to pay attention not only the quantity but also quality of pollen for floral visitors. The recommended way determine value both protein concentration and amino acid composition in insect’s hemolymph. In addition, includes lipids, sterols biogenic elements such as carbon, nitrogen, etc. Very high observed aloe pollen, averaging 51%. Plants with a content, at level 27% Europe, are rapeseed phacelia. turn, plant that poor (at 11%) buckwheat....

Abstract We examined the influence of curcumin-supplemented feeding on worker lifespan, Nosema resistance, key enzyme activities, metabolic compound concentrations and percentage global DNA methylation. Two groups (Apis mellifera) were set up: 1) control group; workers fed ad libitum with sucrose syrup; 2) syrup addition curcumin. Dead removed every two days spp. infection levels assessed. Hemolymph was taken from living for biochemical analyses. The methylation level analysed using heads...

Development of urban agglomerations and the intensification agriculture profoundly affect bees' food resources, hence ecosystem services such as pollination. A solitary bee, Osmia bicornis (syn. O. rufa), is an effective springtime pollinator crops, decorative wild plants. However, it largely unknown if this species conservative or plastic in pollen collection different environments. New breedings were established localizations qualified (90 % built-up infrastructure), suburban (55–65 %),...

Natural bioactive preparations that will boost apian resistance, aid body detoxification, or fight crucial bee diseases are in demand. Therefore, we examined the influence of coenzyme Q10 (CoQ10, 2,3‐dimethoxy, 5‐methyl, 6‐decaprenyl benzoquinone) treatment on honeybee lifespan, Nosema activity/concentration antioxidants, proteases and protease inhibitors, biomarkers. CoQ10 slows age‐related metabolic processes. Workers consumed lived longer than untreated controls were less infested with...

Neonicotinoids, including imidacloprid, harm Apis mellifera in a number of ways, among others by impairing body maintenance and immunity. Energy resources are important to prevent this, particularly, as we hypothesized, the fat tissue. But hormesis (adaptative, diaphasic response stressor) was also reported energy-dependent traits bees exposed sublethal doses imidacloprid. Consequently, concentrations/activities respiratory citric cycle compounds (Acetyl-CoA, IMH-2, AKG, succinate, fumarate,...

Inflammatory bowel disease (IBD) mainly includes ulcerative colitis (UC) and Crohn's (CD). These diseases are classified as chronic recurrent inflammatory affecting the digestive tract. An energy deficiency in intestinal cells is believed to be associated with IBD pathology. Our study investigated bioenergetic functionality of mitochondria using plasma patients CD UC by determining concentration intermediates tricarboxylic acid cycle (TCA), such acetyl coenzyme A, succinate, fumarate,...

DNA methylation is an epigenetic modification that plays important role in the normal development and function of organisms. The level species-, tissue-, organelle-specific, pattern determined during embryogenesis. has also been correlated with age. aim this study was to determine global levels their correlation age chicken, using a Polish autosexing chicken breed, Polbar. A quantitative technique based on immunoenzymatic assay used for analysis. results show increased older Polbar embryos....

The aim of the present study was to establish whether markers oxidative stress and enzymatic defense system blood are related moderate physical activity in younger old oldest men. They were divided into four groups according age level (groups YN YA—inactive active men aged 65-74 years, ON OA—inactive 90-99 years). Venous collected from subjects morning before breakfast. MDA concentration antioxidant enzyme activities (SOD, CAT, GPx, GR) erythrocyte hemolysates assayed. isoprostanes...

Evolution has created different castes of females in eusocial haplodiploids. The difference between them lies their functions and vulnerability but above all reproductive potentials. Honeybee queens are highly fertile. On the other hand, workers facultatively sterile. However, rebel workers, i.e. that develop a queenless colony, reproduce more often than normal workers. As result, fat body these bees, which apart from acting as energy reserve, is also site numerous metabolic processes, had...

This study is a continuation of innovative research on the impact simultaneous use standard- and small-cell combs in bee colonies characteristics worker bees colonies. The nests these had two types combs: (approximate cell width/size 4.90 mm) standard-cell 5.50 mm). aim was to compare activities proteases their inhibitors present cuticle workers reared (SMC workers) (STC kept simultaneously combs. comb cells which were significant effect protein concentrations proteolytic system, i.e....

Aging is a multifactorial process that occurs in all living organisms, including bees. One of the factors accelerating this stress caused bees by Varroa destructor. The research aim was to compare antioxidant system activities different tissues and fat body segments (sternite, tergite 3 5) workers aging naturally (physiologically) prematurely (affected V. destructor). CAT, GPx, GST, SOD were higher tissues/fat age groups compared workers. These increased with age, reaching maximum at 21 (in...

We examined age-related biochemical and histological changes in the fat bodies hemolymph of Osmia rufa males females. analysed solitary bees during diapause, October April; as well flying insects following May June. The trophocyte sizes, numbers lipid droplets were greatest at beginning diapause. Subsequently, they decreased along with age. Triglyceride glucose concentrations systematically body cells but increased from to Concentrations/activities (enzymatic non-enzymatic) antioxidant...

Proper bioelement content is crucial for the health and wellness of all organisms, including honeybees. However, situation more complicated in these important pollinators due to fact that they change their physiology during winter order survive relatively harsh climatic conditions. Additionally, honeybees are susceptible many diseases such as nosemosis, which can depopulate an entire colony. Here we show summer bees have a markedly higher bioelements as: Al, Cu, P, V, (physiologically...

Abstract We examined the influence of bromfenvinphos, a commonly used acaricide, on activities many metabolic enzymes affecting biochemical defences/physiology western honeybee, Apis mellifera L. (Hymenoptera: Apidae), as well some compound concentrations, percentage global DNA methylation, and Nosema spp. infection levels. Bromfenvinphos‐treated workers had decreased haemolymph volumes higher protein concentrations their cuticle but lower in haemolymph. They methylation levels independent...

Abstract Imidacloprid (IMD) may affect proteolysis, aspartate aminotransferase (AST), alanine (ALT), alkaline phosphatase (ALP), and global DNA methylation in honeybees. Queens, drones, workers aged 1 or 20 days were exposed (free-flying colonies) to IMD (5 ppb 200 ppb) their diet. As a result, the colony depopulation did not occurred. disturbed hemolymph/cuticle proteolysis; deactivated most of cuticle protease inhibitors, activated hemolymph thiol metal proteases proteases; downregulated...

Pesticides impair honeybee health in many ways. Imidacloprid (IMD) is a pesticide used worldwide. No information exists on how IMD impact the bees' body bioelement balance, which essential for bee health. We hypothesized that disturbs this balance and fed bees (in field conditions) with diets containing 0 ppb (control), 5 (sublethal considered field-relevant), 200 (adverse) doses of IMD. severely reduced levels K, Na, Ca, Mg (electrolytic) Fe, Mo, Mn, Co, Cu, Ni, Se, Zn, while those Sn, V,...

Rodents are known to play a significant role as reservoir hosts for TBEV. During three sequential expeditions at 4-year intervals ecologically similar study sites in NE Poland, we trapped bank voles (Myodes glareolus) and then tested their blood the presence of specific antiviral antibodies The strongest effects on seroprevalence were extrinsic factors, site capture year sampling. Seroprevalence increased markedly with increasing host age, our analysis revealed interactions among these...

Abstract Many factors, including pathogens, environmental change and breeding techniques, affect honeybee immunity/resistance, so substances natural supplements that enhance it are desired. To eliminate the impact of unknown external in 2016 a cage experiment was conducted under constant laboratory conditions (35 °C, 65% relative humidity). Bees control group were fed with sugar dissolved water at ratio 1:1 ad libitum no additives, while other syrup (1:1) supplemented piperine (3 µg/ml) ....