A5034896242- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Stress Responses and Cortisol
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Axon Guidance and Neuronal Signaling
University of Helsinki
2017-2021
It is unclear how binding of antidepressant drugs to their targets gives rise the clinical effect. We discovered that transmembrane domain tyrosine kinase receptor 2 (TRKB), brain-derived neurotrophic factor (BDNF) promotes neuronal plasticity and responses, has a cholesterol-sensing function mediates synaptic effects cholesterol. then found both typical fast-acting antidepressants directly bind TRKB, thereby facilitating localization TRKB its activation by BDNF. Extensive computational...
Primary neurons from rodent brain hippocampus and cortex have served as important tools in biomedical research over the years. However, protocols for preparation of primary vary, which often lead to conflicting results. This report provides a robust reliable protocol production neuronal cultures with minimal contribution non-neuronal cells. The were grown serum-free media maintained several weeks without any additional feeder according this differentiate by 3 develop extensive axonal...
Abstract Neurotrophins and their receptors have highly conserved evolutionary lineage in vertebrates including zebrafish. The NTRK2 receptor has two isoforms zebrafish, Ntrk2a Ntrk2b. spatio-temporal expression pattern of bdnf ntrk2b the zebrafish brain was studied using situ hybridization. robust corresponding to suggests that is key for brain, unlike its duplicate isoform ntrk2a . To study function, different genetic strategies, TILLING mutant morpholino oligonucleotides (MO), were used....
Neurotrophin brain-derived neurotrophic factor (BDNF) and neurotransmitter serotonin (5-HT) regulate each other have been implicated in several neuronal mechanisms, including neuroplasticity. We investigated the effects of BDNF on serotonergic neurons by deleting receptor TrkB from adult brain. The transgenic mice show increased 5-HT Tph2 levels with abnormal behavioral phenotype. In spite food intake, are significantly leaner than their wildtype littermates, which may be due to metabolic...
Abstract It is unclear how binding of antidepressant drugs to their targets gives rise the clinical effect. We discovered that transmembrane domain TRKB, brain-derived neurotrophic factor (BDNF) receptor promotes neuronal plasticity and responses, has a cholesterol-sensing function mediates synaptic effects cholesterol. then found both typical fast-acting antidepressants directly bind thereby facilitating localization TRKB its activation by BDNF. Extensive computational approaches including...
Abstract Primary neurons from rodent brain hippocampus and cortex has served as great tools in biomedical research over the years. However, protocols for preparation of primary vary, which often leads to conflicting results. This report provides a robust reliable protocol production neuronal cultures with minimal contribution non-neuronal cells. The were grown serum free media maintained several weeks without any additional feeder according this differentiate by three develop extensive...
Abstract Brain-derived neurotrophic factor (BDNF), together with its cognate receptor tyrosine kinase B (TrkB), plays an essential role in the development and plasticity of brain is widely implicated psychiatric diseases (Autry Monteggia, 2012). Due to highly conserved evolutionary lineage neurotrophins their receptors vertebrates, zebrafish a well-suited model for this study. The TrkB receptor, also known as NTRK2, has two forms zebrafish, Ntrk2a Ntrk2b. spatio-temporal expression pattern...