A5061176617- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Zebrafish Biomedical Research Applications
- Mast cells and histamine
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Tryptophan and brain disorders
- Neurotransmitter Receptor Influence on Behavior
- Nerve injury and regeneration
- Treatment of Major Depression
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- Olfactory and Sensory Function Studies
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neural and Behavioral Psychology Studies
- Oral Health Pathology and Treatment
- Phosphodiesterase function and regulation
- Polyamine Metabolism and Applications
- Sleep and related disorders
- Pluripotent Stem Cells Research
- Regulation of Appetite and Obesity
- Forensic Toxicology and Drug Analysis
- Adenosine and Purinergic Signaling
- Signaling Pathways in Disease
University of Helsinki
2015-2025
Center for Neurosciences
2016
Emory University
2006-2008
Russian Academy of Sciences
2003-2008
Institute of Evolutionary Physiology and Biochemistry
2008
Abstract Depression is associated with dysregulated circadian rhythms, but the role of intrinsic clocks in mood-controlling brain regions remains poorly understood. We found increased negative loop and decreased positive clock regulators expression medial prefrontal cortex (mPFC) a mouse model depression, subsequent countermodulation by rapid antidepressant ketamine. Selective Bmal1 KO CaMK2a excitatory neurons revealed that functional mPFC an essential factor for development depression-like...
Abstract Many aspects of photoreceptor metabolism are regulated as diurnal or circadian rhythms. The nature the signals that drive rhythms in mouse photoreceptors is unknown. Dopamine amacrine cells retina express core clock genes, leading us to test hypothesis dopamine regulates protein phosphorylation cells. To this end we investigated phosducin, an abundant photoreceptor‐specific phosphoprotein. In mice exposed a daily light–dark cycle, robust phosducin and retinal were observed....
The histaminergic and hypocretin/orexin (hcrt) neurotransmitter systems play crucial roles in alertness/wakefulness rodents. We elucidated the role of histamine wakefulness interaction hcrt larval zebrafish. Translation inhibition histidine decarboxylase (hdc) with morpholino oligonucleotides (MOs) led to a behaviorally measurable decline light-associated activity, which was partially rescued by hdc mRNA injections mimicked receptor H1 (Hrh1) antagonist pyrilamine treatment....
The basal forebrain (BF) is a key structure in regulating both cortical activity and sleep homeostasis. It receives input from all ascending arousal systems particularly highly innervated by histaminergic neurons. Previous studies clearly point to role for histamine as wake-promoting substance the BF. We used vivo microdialysis pharmacological treatments rats study which electroencephalogram (EEG) spectral properties are associated with histamine-induced wakefulness whether this followed...
Abstract Neurotrophins and their receptors have highly conserved evolutionary lineage in vertebrates including zebrafish. The NTRK2 receptor has two isoforms zebrafish, Ntrk2a Ntrk2b. spatio-temporal expression pattern of bdnf ntrk2b the zebrafish brain was studied using situ hybridization. robust corresponding to suggests that is key for brain, unlike its duplicate isoform ntrk2a . To study function, different genetic strategies, TILLING mutant morpholino oligonucleotides (MO), were used....
The product of melatonin oxidation, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK), was synthesized and a method for its determination in biological samples developed. High performance liquid chromatography (HPLC) with fluorescence detection provided good sensitivity selectivity. Wavelengths 350 480 nm were used excitation emission, respectively. Serum retinal homogenates extracted chloroform prior to analysis by HPLC. Endogenous AFMK detected the retina rats but serum concentration this...
Earlier studies in zebrafish have revealed that acutely given ethanol has a stimulatory effect on locomotion fish larvae but the mechanism of this not been revealed. We studied effects concentrations between 0.75 and 3.00% 7-day-old larval (Danio rerio) Turku strain. At 0.75-3% increased swimming speed during first minute. 3% decreased rapidly after minute, whereas at 1.5% prolonged increase was seen. highest concentration dopamine levels significantly 10-min treatment. found upregulates key...
Objective Nitrous oxide (N 2 O) may elicit antidepressant effects after its elimination from the brain. We sought to investigate whether short-term administration of N O triggers power within delta (0.5-4 Hz) and/or theta (4-7 frequency ranges in electroencephalogram (EEG) it´s administration, as we have recently seen such rebound studies conducted on mice. Methods Twenty healthy male participants were randomly allocated receive 50% either continuously for 20 minutes (continuous group) or...
Recent reports have indicated that nonimmune cells can produce low concentrations of histamine. This observation, together with the discovery high‐affinity histamine H4 receptor (H4R), has added additional layers complexity to our understanding signalling. Human oral keratinocytes (HOKs) possess a uniform H4R pattern, which is deranged in lichen planus (OLP). To investigate metabolism and transport HOKs healthy controls patients OLP. Tissue sections cultured primary were studied using...
Abstract Brain histamine is involved in the regulation of sleep–wake cycle and alertness. Despite widespread use mouse as an experimental model, periodic properties major markers histaminergic system have not been comprehensively characterized. We analysed daily levels its first metabolite, 1‐methylhistamine, different brain structures C 57 BL /6 J CBA / strains, m RNA level activity histidine decarboxylase histamine‐ N ‐methyltransferase mice. In strain, release, assessed by vivo...
Nitrous oxide (N
Several lines of evidence suggest a regulatory role histamine in circadian rhythms, but little is known about signaling pathways that would be involved such putative role. The aim this study was to examine whether mediates its effects on the system through Hrh1 or Hrh3 receptors. We assessed both diurnal and free-running locomotor activity rhythms Hrh1-/- Hrh3-/- mice. also determined expression Per1, Per2 Bmal1 genes suprachiasmatic nuclei, several areas cerebral cortex striatum under...
Many mechanisms have been proposed to explain acute antidepressant drug-induced activation of TrkB neurotrophin receptors, but several questions remain. In a series pharmacological experiments, we observed that induced by antidepressants and other drugs correlated with sedation, most importantly, coinciding hypothermia. Untargeted metabolomics pharmacologically dissimilar activating treatments revealed effects on shared bioenergetic targets involved in adenosine triphosphate (ATP) breakdown...
Aim Under natural conditions diurnal rhythms of biological processes the organism are synchronized with each other and to environmental changes by means circadian system. Disturbances latter affect hormonal levels, sleep-wakefulness cycle cognitive performance. To study mechanisms such perturbations animal models subjected artificial photoperiods often used. The goal current was understand effects rhythm disruption, caused a short light-dark regime, on activity cerebral cortex in rodents....
Abstract Objective Nitrous oxide (N2O) may elicit antidepressant effects after its elimination from the brain. We sought to investigate whether N2O trigger slow-wave oscillations in electroencephalogram (EEG) it´s administration, as we have recently seen animal studies. Methods Twenty healthy male participants were randomly allocated receive 50% either continuously for 20 minutes (continuous group) or two 10-minute inhalations (repeated with EEG recording. The subjective of studied...