A5023195052- Zebrafish Biomedical Research Applications
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Receptor Mechanisms and Signaling
- Esophageal Cancer Research and Treatment
- Neuroendocrine regulation and behavior
- Pancreatic function and diabetes
- Cancer, Stress, Anesthesia, and Immune Response
- Sleep and Wakefulness Research
- Mast cells and histamine
- CRISPR and Genetic Engineering
- Radiomics and Machine Learning in Medical Imaging
- Reproductive System and Pregnancy
- Neonatal Respiratory Health Research
- Pluripotent Stem Cells Research
- Neurotransmitter Receptor Influence on Behavior
- Pregnancy and preeclampsia studies
- Preterm Birth and Chorioamnionitis
- Adrenal and Paraganglionic Tumors
- Ginkgo biloba and Cashew Applications
- COVID-19 and healthcare impacts
- Advanced Neuroimaging Techniques and Applications
- Olfactory and Sensory Function Studies
- Fatty Acid Research and Health
- SARS-CoV-2 detection and testing
Helsinki University Hospital
2022-2024
University of Helsinki
2013-2024
Hospital District of Helsinki and Uusimaa
2024
Sahlgrenska University Hospital
2020-2023
University of Gothenburg
2020
Region Västra Götaland
2020
Abstract Apolipoprotein E ε4 allele ( APOE4 ) has been shown to associate with increased susceptibility SARS-CoV-2 infection and COVID-19 mortality in some previous genetic studies, but information on the role of underlying pathology parallel clinical manifestations is scarce. Here we studied association between APOE Finnish biobank, autopsy prospective cohort datasets. In line work, our data 2611 cases showed that carriership associates severe intensive care patients compared non-infected...
Histamine modulates several behaviours and physiological functions, its deficiency is associated with neuropsychiatric disorders. Gestational intake of valproic acid (VPA) linked to autism spectrum disorder (ASD), characterized by impaired sociability stereotypies. VPA effects on the neurochemistry functional morphology histaminergic system in ASD are unclear. Zebrafish highly social, given similarities between zebrafish human neurotransmitter systems, we have studied histamine...
Population-based cohort studies are essential for understanding the pathological basis of dementia in older populations. Previous have shown that limbic-predominant age-related TDP-43 encephalopathy neuropathological change (LATE-NC) increases with age, but there been only a few studies, which investigated this entity population-based setting. Here we studied frequency LATE-NC and its associations other brain pathologies cognition population aged ≥ 85 years. The Vantaa 85+ study includes all...
Earlier studies in zebrafish have revealed that acutely given ethanol has a stimulatory effect on locomotion fish larvae but the mechanism of this not been revealed. We studied effects concentrations between 0.75 and 3.00% 7-day-old larval (Danio rerio) Turku strain. At 0.75-3% increased swimming speed during first minute. 3% decreased rapidly after minute, whereas at 1.5% prolonged increase was seen. highest concentration dopamine levels significantly 10-min treatment. found upregulates key...
Abstract Aim We aimed at identifying potential roles of vesicular monoamine transporter 2, also known as Solute Carrier protein 18 A2 (SLC18A2) (hereafter, Vmat2), in brain regulation, their turnover, behaviour and development using a novel zebrafish model. Methods A strain lacking functional Vmat2 was generated with the CRISPR/Cas9 system. Larval heart rate were monitored. Monoamines metabolites analysed high‐pressure liquid chromatography. Amine synthesising degrading enzymes, genes...
Fetal and early postnatal inflammation have been associated with increased morbidity in extremely preterm infants. This study aimed to demonstrate if postpartum levels of docosahexaenoic acid (DHA) arachidonic (AA) were inflammation. In a cohort 90 infants, DHA AA cord blood, on the first day 7 examined relation systemic inflammation, defined as elevated C-reactive protein (CRP) and/or interleukin-6 (IL-6) within 72 h from birth, or without positive blood culture. Median serum level was 0.5...
Tumor-infiltrating immune cells play a key role in tumor progression. The purpose of this study was to analyze whether the infiltrate predicts benefit from postoperative radiotherapy large randomized breast cancer trial.In SweBCG91RT trial, patients with stage I and II were breast-conserving surgery (BCS) or BCS only followed for median time 15.2 years. primary quantified through two independent methods: IHC gene expression profiling. For analyses, absolute stromal area occupied by CD8+ T...
Sleep-or sleep-like states-have been reported in adult and larval zebrafish using behavioural criteria. These reversible quiescent periods, displaying circadian rhythmicity, have used pharmacological, genetic neuroanatomical studies of sleep-wake regulation. However, one the important criteria for sleep, namely sleep homeostasis, has not demonstrated unequivocally. To study rest homeostasis larvae, we rest-deprived 1-week-old larvae with a novel, ecologically relevant method: flow water....
Preterm infants exposed to chorioamnionitis and with a fetal inflammatory response are at risk for severe neonatal morbidities adverse outcome. Alarmins S100A8, S100A9, S100A12 expressed by myeloid cells have been associated activation monocyte modulation. Aim: To study S100A alarmin expression in cord blood monocytes from term healthy preterm relate results clinical findings, biomarkers protein levels, as well pathways identified differentially regulated genes. Cord CD14+ were isolated...
Women with SLE face an increased risk of adverse pregnancy outcomes compared healthy women, but the underlying immunological mechanisms are unknown. Given recognised association neutrophil activation pathogenesis, we examined whether there is and inflammation in blood placenta relative to pregnancy.At delivery, peripheral blood, maternal-derived intervillous placentas were collected from 12 10 control pregnancies. The proportion low-density granulocytes (LDGs) status LDG normal-density flow...
Monoaminergic neurotransmission is greatly dependent on the function of vesicular monoamine transporter VMAT2, which responsible for loading monoamines into secretory vesicles. The role VMAT2 in histaminergic poorly understood. We studied structure and system larval zebrafish following inhibition by reserpine. found that reserpine treatment reduced histamine immunoreactivity neurons an almost total disappearance histamine-containing nerve fibers dorsal telencephalon habenula, most densely...
Abstract Aim Histamine receptor H3 (HRH3) has substantial neuropharmacological potential. Currently, knockout models of this have been investigated only in mice. We characterized the expression zebrafish and generated a HRH3 line. Using model, we studied role important behaviours. also analysed effect on monoaminergic systems, which not thoroughly any animal model. Methods Generation mutant line using CRISPR ‐Cas9 system. Analysis locomotor social behaviour. Expression was situ...
Evolving evidence has supported the existence of two anatomically distinct Lewy-related pathology (LRP) types. Investigation spinal cord and peripheral LRP can elucidate mechanisms Lewy body disorders origins synuclein accumulation. Still, very few unselected studies have focused on in these regions. Here we analysed cord, dorsal root ganglion, adrenal gland population-based Vantaa 85 + study, including every ≥ years old citizen living city 1991 (n = 601). Samples from (C6-7, TH3-4, L3-4,...
Primary adrenal leiomyosarcoma (PAL) is a rare, high-grade proliferating mesenchymal tumor with considerable risk of metastasis, deriving from the smooth muscle wall central vein, or its tributaries. Roughly 40 patients PAL have been reported in literature. Herein, we present 3 incidentally discovered PAL, along an overview current knowledge on clinical, radiological, and histopathological characteristics PAL.
We studied the social hierarchy in zebrafish and assessed differences neurotransmitters behavior F1 generation offspring of dominant subordinate (Danio rerio). used behavioral assays to study locomotion, ability complete cognitive tasks, interaction aggression. To neurochemical changes, we applied quantitative polymerase chain reaction, high pressure liquid chromatography immunohistochemistry. Social hierarchies were formed both by males females when animals kept same sex pairs dyadic...
Stem and progenitor cells hold great promise for regenerative medicine gene therapy approaches. However, transplantations of living entail a fundamental risk unwanted growth, potentially exacerbated by CRISPR-Cas9 or other genetic manipulations. Here, we describe safety system to control cell proliferation while allowing robust efficient manufacture, without any added elements. Inactivating TYMS, key nucleotide metabolism enzyme, in several lines resulted that proliferate only when...
We assessed the distribution of SARS-CoV-2 at autopsy in 22 deceased persons with confirmed COVID-19. was found by PCR (2/22, 9.1%) and culture (1/22, 4.5%) skull sawdust, suggesting that live virus is present tissues postmortem, including bone. Occupational exposure risk low appropriate personal protective equipment.
Abstract Background Population‐based cohort studies play a crucial role in unraveling the underlying causes of dementia among older individuals. While previous research has indicated an increase limbic‐predominant age‐related TDP‐43 encephalopathy neuropathological change (LATE‐NC) with age, only limited investigations have delved into this phenomenon within population‐based context. In study, we examined prevalence LATE‐NC and its correlations other brain pathologies cognitive function...
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<div>AbstractPurpose:<p>Tumor-infiltrating immune cells play a key role in tumor progression. The purpose of this study was to analyze whether the infiltrate predicts benefit from postoperative radiotherapy large randomized breast cancer trial.</p>Experimental Design:<p>In SweBCG91RT trial, patients with stage I and II were breast-conserving surgery (BCS) or BCS only followed for median time 15.2 years. primary quantified through two independent methods: IHC gene...
<div>AbstractPurpose:<p>Tumor-infiltrating immune cells play a key role in tumor progression. The purpose of this study was to analyze whether the infiltrate predicts benefit from postoperative radiotherapy large randomized breast cancer trial.</p>Experimental Design:<p>In SweBCG91RT trial, patients with stage I and II were breast-conserving surgery (BCS) or BCS only followed for median time 15.2 years. primary quantified through two independent methods: IHC gene...