A5005828452- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- Prostate Cancer Treatment and Research
- Breast Cancer Treatment Studies
- Chemokine receptors and signaling
- Immune cells in cancer
- Prostate Cancer Diagnosis and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Electrohydrodynamics and Fluid Dynamics
- Breast Lesions and Carcinomas
- Cancer-related gene regulation
- Plant Surface Properties and Treatments
- Advanced Biosensing Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- Cytokine Signaling Pathways and Interactions
- Surface Modification and Superhydrophobicity
- Radiomics and Machine Learning in Medical Imaging
- Micro and Nano Robotics
- Palliative Care and End-of-Life Issues
Exact Sciences (United States)
2022-2024
University of California, San Francisco
2018-2021
Genome British Columbia
2018-2021
University of Michigan
2009-2020
UCSF Helen Diller Family Comprehensive Cancer Center
2018
Michigan United
2014-2015
Ann Arbor Center for Independent Living
2014
There is a clear need for molecular subtyping approach in prostate cancer to identify clinically distinct subgroups that benefit from specific therapies.To subtypes based on luminal and basal lineage determine associations with clinical outcomes response treatment.The PAM50 classifier was used subtype 1567 retrospectively collected (median follow-up, 10 years) 2215 prospectively samples into luminal- basal-like subtypes.Metastasis, biochemical recurrence, overall survival, cancer–specific...
Immunotherapy has been less successful in treating prostate cancer than other solid tumors. We sought to better understand the immune landscape and identify immune-related biomarkers potential therapeutic targets.We analyzed gene expression data from 7826 prospectively collected prostatectomy samples (2013-2016), 1567 retrospective with long-term clinical outcomes, for a total of 9393 samples, all profiled on same commercial platform CLIA-certified lab. The primary outcome was distant...
PURPOSE Adjuvant radiotherapy (RT) is used for women with early-stage invasive breast cancer treated breast-conserving surgery. However, some low risk of recurrence may safely be spared RT. This study aimed to identify these using a molecular-based approach. METHODS We analyzed two randomized trials node-negative ± RT following surgery: SweBCG91-RT (stage I-II, no adjuvant systemic therapy) and Princess Margaret (age 50 years or older, T1-T2, tamoxifen). Transcriptome-wide profiling was...
The Androgen Receptor (AR) is the key-driving transcription factor in prostate cancer, tightly controlled by epigenetic regulation. To date, most profiling has been performed cell lines or limited tissue samples. Here, to comprehensively study landscape, we perform RNA-seq with ChIP-seq for AR and histone modification marks (H3K27ac, H3K4me3, H3K27me3) 100 primary carcinomas. Integrative molecular subtyping of five data streams revealed three major subtypes which two were clearly TMPRSS2-ERG...
PURPOSE Most patients with early-stage breast cancer are treated adjuvant radiotherapy (RT) after breast-conserving surgery (BCS) to prevent locoregional recurrence (LRR). However, no genomic tools used currently select the optimal RT strategy. METHODS We profiled transcriptome of primary tumors on a clinical grade assay from SweBCG91-RT trial, in which node-negative were randomly assigned either whole-breast BCS or RT. derived new classifier, Adjuvant Radiotherapy Intensification Classifier...
Colorful columns: A simple yet scalable method that yields multicompartmental microcylinders with controllable internal architectures, aspect ratios, and controlled surface modification uses electrohydrodynamic co-spinning followed by microsectioning. Compartments are discriminated different colored dyes (see CLSM images; scale bars = 10.0 microm).
Abstract Purpose: Carcinomas originate from epithelial tissues, which have apical (luminal) and basal orientations. The degree of luminal versus differentiation in cancer has been shown to be biologically important some carcinomas impacts treatment response. Experimental Design: Although prior studies focused on individual types, we used a modified clinical-grade classifier (PAM50) subtype 8,764 tumors across 22 different into A, B, basal-like tumors. Results: We found that all demonstrated...
Luminal and basal subtypes of primary prostate cancer have been shown to be molecularly distinct clinically important in predicting response therapy. These not described metastatic cancer.To identify clinical molecular correlates luminal castration-resistant (mCRPC) investigate differences survival, particularly after treatment with androgen-signaling inhibitors (ASIs).In this cohort study, a retrospective analysis was conducted 4 cohorts mCRPC (N = 634) across multiple academic centers....
In murine macrophages stimulated with macrophage-colony-stimulating factor (M-CSF), signals essential to macropinosome formation are restricted the domain of plasma membrane enclosed within cup-shaped, circular ruffles. Consistent a role for these actin-rich structures in signal amplification, microscopic measures Rac1 activity determined that disruption actin polymerization by latrunculin B inhibited ruffling and localized activation response M-CSF. To test hypothesis ruffles restrict...
Chemokine CXCL12 promotes growth and metastasis of more than 20 different human cancers, as well pathogenesis other common diseases. binds two receptors, CXCR4 CXCR7, both which recruit signal through the cytosolic adapter protein β-arrestin 2. Differences in CXCL12-dependent recruitment 2 cells expressing one or receptors remain poorly defined. To quantitatively investigate parameters controlling association with CXCR7 co-expressing we used a systems biology approach combining real-time,...
A substantial number of patients diagnosed with high-risk prostate cancer are at risk for metastatic progression after primary treatment. Better biomarkers needed to identify the highest guide therapy intensification.To create a DNA damage and repair (DDR) pathway profiling method use as prognostic signature biomarker in cancer.A cohort 1090 who underwent prostatectomy were treated 3 different academic institutions divided into training (n = 545) pooled validation cohorts 232, 130, 183)...
CXCL12-CXCR4-CXCR7 signaling promotes tumor growth and metastasis in breast cancer. Alternative splicing of CXCL12 produces isoforms with distinct structural biochemical properties, but little is known about isoform-specific differences cancer subtypes patient outcomes. We investigated global expression profiles the six isoforms, CXCR4, CXCR7 The Cancer Genome Atlas cohort using next-generation RNA sequencing 948 benign samples seven cell lines. compared levels several clinical parameters,...
The trend toward precision-based therapeutic approaches dictated by molecular alterations offers substantial promise for men with metastatic castration-resistant prostate cancer (mCRPC). However, current characterization are primarily tissue based, necessitating serial biopsies to understand changes over time and limited the challenges inherent extracting genomic material from predominantly bone metastases. Therefore, a circulating tumor cell (CTC)-based assay was developed determine gene...
Chemokines critically regulate chemotaxis in normal and pathologic states, but there is limited understanding of how multicellular interactions generate gradients needed for cell migration. Previous studies CXCR4+ cells toward chemokine CXCL12 suggest the requirement expressing scavenger receptor CXCR7 a source-sink system. We leveraged an established microfluidic device to discover that CXCR4 distinct isoforms required scavenging only under conditions with higher than optimal levels CXCL12....
Currently, no genomic signature exists to distinguish men most likely progress on adjuvant androgen deprivation therapy (ADT) after radical prostatectomy for high-risk prostate cancer. Here we develop and validate a gene expression predict response postoperative ADT.
Chemokine gradient formation requires multiple processes that include ligand secretion and diffusion, receptor binding internalization, immobilization of to surfaces. To understand how these events dynamically shape gradients influence ensuing cell chemotaxis, we built a multi-scale hybrid agent-based model linking formation, responses, receptor-level information. The CXCL12/CXCR4/CXCR7 signaling axis is highly implicated in metastasis many cancers. We CXCL12 as it impacted by CXCR4 CXCR7,...
Protein kinases are known to play a prominent role in oncogenic progression across multiple cancer subtypes, yet their prostate remains underexplored. The purpose of this study was identify that drive progression.
Tumor-infiltrating immune cells play a key role in tumor progression. The purpose of this study was to analyze whether the infiltrate predicts benefit from postoperative radiotherapy large randomized breast cancer trial.In SweBCG91RT trial, patients with stage I and II were breast-conserving surgery (BCS) or BCS only followed for median time 15.2 years. primary quantified through two independent methods: IHC gene expression profiling. For analyses, absolute stromal area occupied by CD8+ T...
Abstract Farbenprächtige Säulen : Eine einfache und dabei skalierbare Methode zur Herstellung multikompartimentalisierter Mikrozylinder, bei denen innerer Aufbau, Seitenverhältnis Oberfläche gezielt eingestellt werden können, nutzt elektrohydrodynamisches Spinnen Mikroabtrennen. Die Kompartimente mithilfe verschiedenfarbiger Farbstoffe unterschieden (siehe CLSM‐Bilder; Maßstab: 10 μm). magnified image
Abstract Purpose: There is currently no molecular signature in clinical use for adjuvant endocrine therapy omission breast cancer. Given the unique trial design of SweBCG91-RT, where and chemotherapy were largely unadministered, we sought to evaluate potential transcriptomic profiling identifying patients who may be spared therapy. Experimental Design: We performed a whole-transcriptome analysis randomized phase III ± radiotherapy after breast-conserving surgery node-negative stage I–IIA...
Biomolecular interactions between proteins and synthetic surfaces impact diverse biomedical fields. Simple, quantitative, label-free technologies for the analysis of protein adsorption binding biomolecules are thus needed. Here, we report use a novel type substrate, poly-p-xylylene coatings prepared by chemical vapor deposition (CVD) polymerization, surface plasmon resonance enhanced ellipsometry (SPREE) studies assess reactive as spatially resolved biomolecular sensing arrays. Prior to in...