A5005763628- Heart Rate Variability and Autonomic Control
- Neuroscience of respiration and sleep
- Cardiovascular Disease and Adiposity
- Cardiovascular Health and Disease Prevention
- Spaceflight effects on biology
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Nuclear Receptors and Signaling
- High Altitude and Hypoxia
- Neonatal Respiratory Health Research
- Connexins and lens biology
- Retinal Development and Disorders
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Thermoregulation and physiological responses
- Preterm Birth and Chorioamnionitis
- Galectins and Cancer Biology
- Biochemical effects in animals
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- Neonatal and Maternal Infections
- Macrophage Migration Inhibitory Factor
- Retinopathy of Prematurity Studies
- Renal Diseases and Glomerulopathies
University of Gothenburg
2008-2024
Lomonosov Moscow State University
1998-2020
Although the biophysical fingerprints (ion selectivity, voltage-dependence, kinetics, etc) of Ca(2+)-activated Cl(-) currents are well established, their molecular identity is still controversial. Several candidates have been suggested; however, none them has fully accepted. We recently characterized a cGMP-dependent current with unique characteristics in smooth muscle cells. This novel shown to coexist "classic" (cGMP-independent) and distinct from those previously known for currents. Here,...
Preterm infants exposed to chorioamnionitis and with a fetal inflammatory response are at risk for severe neonatal morbidities adverse outcome. Alarmins S100A8, S100A9, S100A12 expressed by myeloid cells have been associated activation monocyte modulation. Aim: To study S100A alarmin expression in cord blood monocytes from term healthy preterm relate results clinical findings, biomarkers protein levels, as well pathways identified differentially regulated genes. Cord CD14+ were isolated...
Abstract Background Cytokines and growth factors (GF) have been implicated in the development of retinopathy prematurity (ROP) bronchopulmonary dysplasia (BPD). We hypothesize that even small coordinated changes inflammatory proteins or GFs may reveal underlying regulating mechanisms do not induce obvious concentration individual proteins. therefore applied correlation network analysis serum to determine early characteristics these conditions. Methods Concentrations 17 cytokines five were...
Myeloperoxidase (MPO) activity is suggested to reduce the function of vascular nitric oxide, thereby contributing endothelial dysfunction, although data in rodents are inconclusive. We examined contractile and relaxant responses MPO-deficient (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mtext mathvariant="normal">M</mml:mtext><mml:mtext mathvariant="normal">P</mml:mtext><mml:mtext...
Bestrophins are putative calcium-activated chloride channels. Recently, cell-protective functions for Bestrophin-3 (Best3) were proposed. Best3 exists in different splice variants. We have here examined expression, alternative splicing and localization of mouse podocytes under normal conditions during endoplasmic reticulum (ER) stress.Best3 expression was determined on the mRNA level using quantitative PCR protein by immunohistochemistry Western blotting.Staining pronounced glomeruli...
Bestrophin-3, a potential candidate for calcium-activated chloride channel, recently was suggested to have cell-protective functions. We studied the expression and alternative splicing of bestrophin-3 in neonatal mouse brain after hypoxic-ischemic (HI) injury human samples. HI induced 9-day old mice by unilateral permanent common carotid artery occlusion combination with exposure 10% oxygen 50 min. Endoplasmic reticulum stress thapsigargin treatment primary culture astrocytes. also...
Introduction: Mechanical forces and sympathetic influences are key determinants of vascular structure function. This study tested the hypothesis that hindlimb unloading (HU) exert diverse effects on rat forelimb small arteries in functionally different regions skeletal muscle skin. Methods: Male Wistar rats were subjected to HU for 2 weeks, then (deep brachial sural) skin (median saphenous) examined in-vitro using wire myography or isobaric perfusion glyoxylic acid staining. Results:...
Arteries display cyclic diameter variations, vasomotion. In vivo, these rhythmic contractions are modulated by the influence of sympathetic nerves. this study, we investigated effect burst stimulation intramural nerves in vitro on vasomotion rat mesenteric small arteries. Vessels were mounted for isometric force measurement. After initiation with noradrenaline (0.5–2 µ<i>M</i>), periarterial stimulated electrically (10 impulses at 20 Hz) approximately half-minute intervals. With...
Microglia may contribute to injury but also have neuroprotective properties. Galectin-3 has immunomodulatory properties that affect the microglia phenotype and subsequent development of injury. contributes experimental hypoxic-ischemic (HI) in neonatal brain, it is unclear if galectin-3 similar effects on infectious sterile inflammation. Thus, we investigated effect vitro under normal as well inflammatory conditions, brain following an challenge vivo. Conditions mimicking or inflammation...
Bestrophins, named after their association with Best’s disease (vitelliform macular dystrophy) are transmembrane proteins that function as calcium‐activated chloride channels (CaCC's), but may also play a role in cell survival. Bestrophins exist several isoforms. Bestrophin‐3 is cyclic‐GMP‐regulated CaCC vascular smooth muscle. Its the intestine has not been studied, another isoform, bestrophin‐2, present or bicarbonate transporter goblet cells of colon. We have here determined expression...
Since the cardiovascular consequences of obesity reportedly vary in different types obesity, we investigated influence adipose tissue from locales on phenylephrine-induced tone mouse carotid artery. Vessels were mounted a Mulvany-Halpern-type wire myograph, and tissue, back (brown) or mesenteric inguinal subcutaneous (white), was placed around Contractile responses to phenylephrine not affected by brown but reduced (<i>p</i> < 0.001) either type white with no difference...
The bestrophin isoform, bestrophin‐3 (Best3), acts as a calcium‐activated chloride channel in cardiomyocytes and vascular smooth muscle. It may have cell‐protective functions other cell types. Best3 be regulated by cyclic GMP exists different splice variants. We examined expression localization of mouse kidney on the mRNA level using RT‐PCR protein immunohistochemistry Western blotting. Staining for was obtained glomeruli, also detected cultured podocytes. blot confirmed presence cortex. did...
Perivascular adipose tissue (PVAT) secretes the dilator substance “adipocyte‐derived‐relaxing‐factor” (ADRF) that has been shown to act mainly through voltage‐dependent (KV) and ATP‐dependent (KATP) potassium channels (K). Malfunction in ADRF may contribute metabolic syndrome. A recently suggested candidate is hydrogen sulfide (H2S) generated by enzymes CSE, CBS 3MST. We have here investigated effect of H2S from three different kinds tissues (AT) on mouse carotid arteries, probed mechanisms...