A5073542350- Plant Molecular Biology Research
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Microbial Community Ecology and Physiology
- Plant Reproductive Biology
- Aquaculture disease management and microbiota
- Chromosomal and Genetic Variations
- Viral-associated cancers and disorders
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant and animal studies
- Cytomegalovirus and herpesvirus research
- Antimicrobial Peptides and Activities
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Genetic Mapping and Diversity in Plants and Animals
- Gut microbiota and health
- Plant Parasitism and Resistance
- Immune Response and Inflammation
- Polyomavirus and related diseases
- Insects and Parasite Interactions
- HIV Research and Treatment
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Cancer, Stress, Anesthesia, and Immune Response
Center for Cancer Research
2023-2025
National Cancer Institute
2023-2025
National Centre for Biological Sciences
2017-2024
Tata Institute of Fundamental Research
2018-2024
SASTRA University
2017-2023
MicroRNAs are a class of non-coding small RNAs involved in the negative regulation gene expression, which play critical roles developmental and metabolic pathways. Studies several plants have identified few microRNAs other that target regulators phenylpropanoid pathway called MYB transcription factors. However, it is not well understood how sRNA-mediated MYBs influences accumulation specific secondary metabolites. Using sRNA sequencing, degradome analysis, mRNA proteomic we establish grape...
Abstract Domestication of rice (Oryza sativa) included conversion perennial wild species with few seeds to short plants that produced abundant seeds. Most domestication-associated changes were due variations in transcription factors and other key proteins such as enzymes. Here, we show multiple yield-related traits associated indica domestication are linked micro (mi) RNA-mediated regulation. Analysis small (s) RNA data sets from cultivated lines, a landraces, two relatives revealed the...
Abstract Micro (mi)RNAs are 20–22nt long non-coding RNA molecules involved in post-transcriptional silencing of targets having high base-pair complementarity. Plant miRNAs processed from Pol II-transcripts with specific stem-loop structures by Dicer-like (DCL) 1 protein. Although there were reports indicating how a region is selected for miRNA biogenesis, molecular details unclear. Here, we show that the presence GC-rich sequence signature within miRNA/miRNA* required precise biogenesis. The...
The spindle cells of Kaposi sarcoma (KS) lesions primarily express herpesvirus (KSHV) latent genes with minimal expression lytic genes. However, recent transcriptome analyses KS have shown high KSHV open reading frame (ORF) 75, which is considered a late gene based on in primary effusion lymphoma (PEL) lines. ORF75 encodes pseudo-amidotransferase that part the viral tegument, acts as suppressor innate immunity, and essential for replication. We assessed representative lesion by RNAscope...
Abstract Genetic variation at the 8q24 locus is linked with greater susceptibility to prostate cancer in men of African ancestry. One such ancestry specific rare variant, rs72725854 (A>G/T) (~6% allele frequency) has been associated a ~2-fold increase risk. However, functional relevance this variant unknown. Here we show that present cancer-specific enhancer locus. Chromatin-conformation capture and dCas9 mediated blocking establish direct regulatory link between lncRNAs PCAT1, PRNCR1...
Chloroplast is the site for transforming light energy to chemical energy. It also acts as a production unit variety of defense-related molecules. These defense moieties are necessary mount successful counter against pathogens, including viruses. Previous studies indicated disruption chloroplast homeostasis basic strategy Begomovirus its infection leading vein-clearing, mosaic, and chlorotic symptoms in infected plants. Although begomoviral pathogenicity determinant protein Beta C1 (βC1) was...
Host-associated gut microbiota can have significant impacts on host ecology and evolution are often host-specific. Multiple factors contribute to such host-specificity: (1) dietary specialization passively determining microbial colonization, (2) hosts selecting for specific diet-acquired microbiota, or (3) a combination of both. The latter possibilities indicate functional association should produce stable microbiota. We tested these alternatives by analyzing the bacterial communities six...
Wounding is a general stress in plants that results from various pest and pathogenic infections addition to environment-induced mechanical damages. Plants have sophisticated molecular mechanisms recognize respond wounding, with those of monocots being distinct dicots. Here, we show the involvement two categories temporally separated, endogenously derived peptides, namely, plant elicitor peptides (PEPs) phytosulfokine (PSK), mediating wound responses rice. These trigger dynamic signal relay...
Abstract Background To successfully invade new hosts, plant viruses must break host resistance and be competent to move within between cells. As a means, viral proteins known as pathogenicity determinants have evolved coordinate network of protein interactions. The βC1 encoded by specific geminiviral satellites acts key determinant for this disease-causing family viruses. Post-translational modifications (PTMs) such ubiquitination phosphorylation the been shown occur in diverse However,...
Summary RNA‐dependent RNA polymerases (RDR) generate double‐stranded (ds)RNA triggers for silencing across eukaryotes. Among the three clades, α‐clade and β‐clade members are key components of mediators stress responses However, γ‐clade unusual in that they represented phylogenetically distant plants fungi, their functions unknown. Using genetic, bioinformatic biochemical methods, we show RDRs from Oryza sativa L. involved plant development as well regulation expression coding noncoding...
Abstract Epstein-Barr virus (EBV) downregulates immune surface markers to avoid recognition. Pomalidomide (Pom) was previously shown increase marker expression in EBV-infected tumor cells. We explored the mechanism by which Pom leads these effects increased B7-2/CD86 mRNA, protein, and cells but this virtually eliminated made resistant Pom-induced cytostatic effects. This indicates that initiates upregulation of interacting with its target, cereblon. Interestingly, proinflammatory cytokines...
ABSTRACT Host-associated gut microbial communities can have large impacts on host ecology and evolution, are typically shaped by taxonomy diet. Different species often harbor distinct communities, potentially because (1) dietary specialization determines colonization, (2) host-specific selection acts diet-acquired microbiota, (3) a combination of both processes. While the first possibility involves passive community structuring, other two may arise from functional association should produce...
Tixocortol, the active component in a nasal spray, inhibits SARS-CoV-2 main protease and viral replication. This highlights its potential for use preventing and/or treating infections.
The spindle cells of Kaposi sarcoma (KS) lesions primarily express herpesvirus (KSHV) latent genes with minimal expression lytic genes. However, recent transcriptome analyses KS have shown high KSHV open reading frame (ORF) 75, which is considered a late gene based on in primary effusion lymphoma (PEL) lines. ORF75 encodes pseudo-amidotransferase that part the viral tegument, acts as suppressor innate immunity, and essential for replication. We assessed representative lesion by RNAscope...
Abstract Wounding is a general stress in plants that results from various pest and pathogenic infections addition to environment induced mechanical damages. Plants have sophisticated molecular mechanisms recognize respond pests pathogens. Although several molecules such as phytohormones, peptides receptors been attributed wound responses dicots, for monocots probably having distinct are less understood. Here, we show the involvement of two categories temporally separated, endogenously...
MicroRNAs (miRNAs) are small ~21nt long endogenous non-coding RNAs that involved in post-transcriptional silencing of target mRNAs.miRNAs processed from precursors having a stem-loop structure recognized and cleaved by Dicer-like1 (DCL1) with the help dsRNA binding protein Hyponastic leaves1 (HYL1) zinc finger named Serrate (SE).Unlike animal miRNAs where miRNA foldbacks mostly uniform, plant very diverse length structure.Processing mechanism nature RNA motifs pre-miRNAs comparatively well...
Abstract To successfully invade new hosts, to break host resistance as well move within and between plant cells, viruses their satellites have evolved a coordinated network of protein interactions. βC1 encoded by specific geminiviral acts key pathogenicity determinant. from diverse undergo multiple post-translational modifications (PTMs) such ubiquitination phosphorylation. However, the relevance these other layers PTMs in host-geminiviral interactions has not been fully understood. Here we...
Abstract Chloroplast is the site for transforming light energy to chemical energy. It also acts as a production unit variety of defense-related molecules. These defense moieties are necessary mount successful counter defence against pathogens including viruses. Geminiviruses disrupt chloroplast homeostasis basic strategy their infection inducing vein-clearing, mosaics and chlorosis in infected plants. Here we show that geminiviral pathogenicity determinant protein βC1 directly interferes...