A5083184462- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Advanced biosensing and bioanalysis techniques
- Corrosion Behavior and Inhibition
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Trace Elements in Health
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Research and Splicing
- Actinomycetales infections and treatment
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Pesticide and Herbicide Environmental Studies
- ATP Synthase and ATPases Research
- Pesticide Exposure and Toxicity
- Radiopharmaceutical Chemistry and Applications
- Plant Reproductive Biology
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
- Plant Molecular Biology Research
- Antimicrobial Resistance in Staphylococcus
National Centre for Biological Sciences
2015-2025
Tata Institute for Genetics and Society
2023
Tata Institute of Fundamental Research
2020-2023
University of Maryland, College Park
2015
The University of Texas Southwestern Medical Center
2009-2014
Texas A&M University
2006-2007
A dual-action RNA switch for expression Riboswitches are short segments of that bind small molecules and between two different conformations, thereby regulating gene (see the Perspective by Chen Gottesman). DebRoy et al. Mellin find a new class riboswitches—in species bacteria—that both part regulate production noncoding RNA. Each riboswitch ensures particular metabolic pathway is only activated in presence an essential small-molecule cofactor. In absence cofactor, full-length non-coding...
Ethanolamine, a product of the breakdown phosphatidylethanolamine from cell membranes, is abundant in human intestinal tract and processed foods. Effective utilization ethanolamine as carbon nitrogen source may provide survival advantage to bacteria that inhabit gastrointestinal influence virulence pathogens. In this work, we describe unique series posttranscriptional regulatory strategies expression genes ( eut ) Enterococcus , Clostridium Listeria species. One these mechanisms requires an...
All living organisms require mechanisms for coordination of intracellular metal concentrations. Many types metal-sensing regulatory proteins have been described previously. Data in recent years revealed that posttranscriptional are also utilized control ion homeostasis bacteria. In particular, two classes RNA structural elements discovered to coordinate magnesium-induced transitions with expression levels downstream genes. We discuss these RNAs herein and compare them other riboswitches...
ANTAR proteins are widespread bacterial regulatory that have RNA–binding output domains and utilize antitermination to control gene expression at the post-initiation level. An protein, EutV, regulates ethanolamine-utilization genes (eut) in Enterococcus faecalis. Using this system, we present genetic biochemical evidence of a general mechanism used by ANTARs, including details antiterminator structure. The novel structure consists two small hairpins with highly conserved terminal loop...
Monitoring dynamic changes in mitochondrial pH live cells using pH-sensitive fluorescent probe.
The persistence of Mycobacterium tuberculosis (Mtb) is a major problem in managing (TB). Host-generated nitric oxide (NO) perceived as one the signals by Mtb to reprogram metabolism and respiration for persistence. However, mechanisms involved NO sensing reorganizing Mtb's physiology are not fully understood. Since damages iron-sulfur (Fe–S) clusters essential enzymes, mechanism(s) regulating Fe–S cluster biogenesis could help persist host tissues. Here, we show that transcription factor...
FMRP is an RNA-binding protein that known to localize in the cytoplasm and nucleus. Here, we have identified interaction of with a specific set C/D box snoRNAs guide 2'O methylations ribosomal RNA (rRNA) on defined sites, this modification regulates rRNA folding assembly ribosomes. methylation partial several sites human embryonic stem cells, which results ribosomes differential patterns. FMRP-snoRNA affects these absence FMRP, pattern significantly altered. We found recognizes carrying...
The continued resurgence of the COVID-19 pandemic with multiple variants underlines need for diagnostics that are adaptable to virus. We have developed toehold RNA-based sensors across SARS-CoV-2 genome direct and ultrasensitive detection virus its prominent variants. Here, isothermal amplification a fragment RNA coupled activation our biosensors leads conformational switch in sensor. This translation reporter protein, example, LacZ or nano-lantern is easily detected using...
Ro ribonucleoproteins are a class of antigenic associated with rheumatic autoimmune diseases like systemic lupus erythematosus and Sjögrens syndrome in humans. mostly composed the 60-kDa protein small cytoplasmic RNAs, called Y unknown function. In eukaryotes, where has been found to associate damaged or mutant it suggested that may play role RNA quality control. radiation-resistant bacterium Deinococcus radiodurans some also implicated cell survival following UV damage. Here we present...
Non-coding RNAs play pivotal roles in bacterial signaling. However, from certain phyla (specially high-GC actinobacteria) still remain elusive. Here, by re-engineering the existing genome-wide search approach, we discover a family of structurally conserved that are present ubiquitously across actinobacteria, including mycobacteria. In vitro analysis shows belonging to this bind response-regulator proteins contain widely prevalent ANTAR domain. The Mycobacterium tuberculosis protein gets...
pH dynamically regulates diverse cellular functions and processes. At the Inner Mitochondrial Membrane (IMM), nanoscale gradients generated by Electron Transport Chain (ETC) play a critical role in contributing to mitochondrial membrane potential that drives ATP synthesis thermogenesis. However, tools decouple from overall IMM living cells are limited. This study integrates fluorescent “benzo-indole” chromophore with pH-sensitive “phenol” moiety into single covalent skeleton build sensitive,...
ABSTRACT The continued resurgence of the COVID-19 pandemic with multiple variants underlines need for diagnostic strategies, that are easily adapatable to changing virus. Here, we have designed and developed toehold RNA-based sensors across SARS-CoV-2 genome direct ultrasensitive detection virus its prominent variants. In our assay, isothermal amplification a fragment RNA coupled activation biosensors leads conformational switch in sensor. This translation reporter-protein e.g. LacZ or...
ABSTRACT Ribosomes, the molecular machines that are central to protein synthesis, have gradually been gaining prominence for their regulatory role in translation. Eukaryotic cytosolic ribosomes typically larger than bacterial ones, partly due multi-nucleotide insertions at specific conserved positions ribosomal RNAs (rRNAs). Such called expansion segments (ESs) present primarily on surface, with translation and its regulation remaining under-explored. One such ES large subunit (LSU) is...
Organophosphate hydrolases (OPH), hitherto known to hydrolyze the third ester bond of organophosphate (OP) insecticides and nerve agents, have recently been shown interact with outer membrane transport components, namely, TonB ExbB/ExbD. In an OPH negative background, Sphingopyxis wildii cells failed ferric enterobactin showed retarded growth under iron-limiting conditions. We now show OPH-encoding degradation (opd) gene from Sphingobium fuliginis ATCC 27551 be part iron regulon. A fur-box...