Rajeev Jha
A5101797317- COVID-19 Clinical Research Studies
- Tuberculosis Research and Epidemiology
- Cancer therapeutics and mechanisms
- Antibiotic Resistance in Bacteria
- Sepsis Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Mycobacterium research and diagnosis
- interferon and immune responses
- Respiratory Support and Mechanisms
- Intensive Care Unit Cognitive Disorders
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Long-Term Effects of COVID-19
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Photovoltaic System Optimization Techniques
- Pneumocystis jirovecii pneumonia detection and treatment
- Energy and Environment Impacts
- Solar Radiation and Photovoltaics
- COVID-19 Impact on Reproduction
- Vitamin C and Antioxidants Research
- Endoplasmic Reticulum Stress and Disease
National Cancer Institute
2021-2024
Barnet Hospital
2021-2023
Royal Free London NHS Foundation Trust
2021-2023
Center for Cancer Research
2023
Indira Gandhi Institute of Medical Sciences
2023
National Institutes of Health
2022-2023
Foundation for Professional Development
2023
The Recovery Center
2022
Indian Institute of Science Bangalore
2005-2021
University of Siena
2021
<h3>Importance</h3> Continuous positive airway pressure (CPAP) and high-flow nasal oxygen (HFNO) have been recommended for acute hypoxemic respiratory failure in patients with COVID-19. Uncertainty exists regarding the effectiveness safety of these noninvasive strategies. <h3>Objective</h3> To determine whether either CPAP or HFNO, compared conventional therapy, improves clinical outcomes hospitalized COVID-19–related failure. <h3>Design, Setting, Participants</h3> A parallel group,...
<h3>Importance</h3> The efficacy of antiplatelet therapy in critically ill patients with COVID-19 is uncertain. <h3>Objective</h3> To determine whether improves outcomes for adults COVID-19. <h3>Design, Setting, and Participants</h3> In an ongoing adaptive platform trial (REMAP-CAP) testing multiple interventions within therapeutic domains, 1557 adult were enrolled between October 30, 2020, June 23, 2021, from 105 sites 8 countries followed up 90 days (final follow-up date: July 26, 2021)....
The longer-term effects of therapies for the treatment critically ill patients with COVID-19 are unknown.
Overactivation of the renin-angiotensin system (RAS) may contribute to poor clinical outcomes in patients with COVID-19.
High-intensity transcription and replication supercoil DNA to levels that can impede or halt these processes. As a potent amplifier accelerator, the proto-oncogene MYC must manage this interfering torsional stress. By comparing gene expression with recruitment of topoisomerases promoters, we surmised direct association topoisomerase 1 (TOP1) TOP2 was confirmed in vitro cells. Beyond recruiting topoisomerases, directly stimulates their activities. We identify MYC-nucleated "topoisome" complex...
The efficacy of vitamin C for hospitalized patients with COVID-19 is uncertain.
Movement of the transcription machinery along a template alters DNA topology resulting in accumulation supercoils DNA. The positive generated ahead transcribing RNA polymerase (RNAP) and negative accumulating behind impose severe topological constraints impeding process. Previous studies have implied role topoisomerases removal torsional stress maintenance but vivo interaction functionally distinct with heterogeneous chromosomal territories is not deciphered. Moreover, how...
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Transcription in the nucleus occurs a concentrated, dense environment, and no reasonable biochemical facsimile of this milieu exists. Such environment would be important for further understanding transcriptional regulation. We describe here formation dense, transcriptionally active bodies vitro with only nuclear extracts promoter DNA. These biomolecular condensates (BMCs) are 0.5 to 1 μm diameter, have macromolecular density approximately 100 mg/ml, consequence phase transition between DNA...
The persistence of Mycobacterium tuberculosis (Mtb) is a major problem in managing (TB). Host-generated nitric oxide (NO) perceived as one the signals by Mtb to reprogram metabolism and respiration for persistence. However, mechanisms involved NO sensing reorganizing Mtb's physiology are not fully understood. Since damages iron-sulfur (Fe–S) clusters essential enzymes, mechanism(s) regulating Fe–S cluster biogenesis could help persist host tissues. Here, we show that transcription factor...
Nucleoid-associated proteins (NAPs) in bacteria contribute to key activities such as DNA compaction, chromosome organization and regulation of gene expression. HU Lsr2 are two principal NAPs Mycobacterium tuberculosis (Mtb). is essential for Mtb survival one the most abundant NAPs. It differs from other eubacterial having a long, flexible lysine- arginine-rich carboxy-terminal domain. functional analogue bacterial NAP commonly called H-NS. binds regulates expression A/T-rich portions...
Energy plays a prominent role in human society. As result of technological and industrial development, the demand for energy is rapidly increasing. Existing power sources that are mainly fossil fuel based leaving an unacceptable legacy waste pollution apart from diminishing stock fuels. Hence, focus now shifted to large-scale propagation renewable energy. Renewable technologies clean have much lower environmental impact than conventional technologies. Solar one such Most comes either...
Abstract Herein, we characterize the cellular uptake of a DNA structure generated by rolling circle amplification. The structure, termed nanoflower, was fluorescently labeled incorporation ATTO488-dUTP allowing intracellular localization to be followed. nanoflower had hydrodynamic diameter approximately 300 nanometer and non-toxic for all mammalian cell lines tested. It internalized specifically macrophages phagocytosis within few hours resulting in specific compartmentalization...
Abstract Gre, one of the conserved transcription factors in bacteria, modulates RNA polymerase (RNAP) activity to ensure processivity and fidelity synthesis. Gre regulate by inducing intrinsic-endonucleolytic RNAP, allowing enzyme resume from paused arrested sites. While Escherichia coli a number eubacteria harbor GreA GreB, genus mycobacteria has single (GreA). To address importance growth, physiology gene expression Mycobacterium smegmatis , we have constructed conditional knock-down...
Nebulized thrombolysis offers locally targeted therapy with potentially lower bleeding risk than systemic administration for coronavirus disease 2019 (COVID-19) respiratory failure. In a proof-of-concept safety study, adult patients COVID-19-induced failure and &lt;300mmHg PaO2/FiO2 (P/F) ratio, requiring invasive mechanical ventilation (IMV) or non-invasive support (NIRS) received nebulized rt-PA in two cohorts (C1 C2), alongside standard of care during the first UK COVID-19 waves....
Acute lung injury in COVID-19 results diffuse alveolar damage with disruption of the alveolar-capillary barrier, coagulation activation, fibrin deposition and pulmonary capillary thrombi. Nebulized recombinant tissue plasminogen activator (rt-PA) has potential to facilitate localized thrombolysis compartment improve oxygenation. In this proof-of-concept safety study, adults COVID-19-induced respiratory failure a <300 mmHg PaO