A5033643030- Hearing, Cochlea, Tinnitus, Genetics
- Neuroscience and Neuropharmacology Research
- Cancer Cells and Metastasis
- Vestibular and auditory disorders
- RNA modifications and cancer
- Adenosine and Purinergic Signaling
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Ion Channels and Receptors
- RNA and protein synthesis mechanisms
- Calcium signaling and nucleotide metabolism
- Biosensors and Analytical Detection
- Biochemical Analysis and Sensing Techniques
- Advanced biosensing and bioanalysis techniques
- Botanical Research and Applications
- RNA regulation and disease
- Synthesis of Organic Compounds
- Microbial Metabolites in Food Biotechnology
- Single-cell and spatial transcriptomics
- Bioactive natural compounds
- Fungal and yeast genetics research
- Estrogen and related hormone effects
- Spaceflight effects on biology
- Streptococcal Infections and Treatments
- Hydrocarbon exploration and reservoir analysis
University of Mississippi Medical Center
2020-2024
Jackson Memorial Hospital
2022-2024
Institute for Stem Cell Biology and Regenerative Medicine
2020-2022
Manipal Academy of Higher Education
2022
Southern Illinois University School of Medicine
2014-2019
West Bengal University of Health Sciences
1988-2018
Louisiana State University Health Sciences Center New Orleans
2004
Louisiana State University
2004
Tulane University
2002
Cancer Research Institute of the Slovak Academy of Sciences
1974
Cisplatin is a commonly used antineoplastic agent that produces ototoxicity mediated in part by increasing levels of reactive oxygen species (ROS) via the NOX3 NADPH oxidase pathway cochlea. Recent studies implicate ROS generation mediating inflammatory and apoptotic processes hearing loss activating signal transducer activator transcription (STAT1). In this study, we show adenosine A 1 receptor (A AR) protects against cisplatin suppressing an response initiated oxidase, leading to...
Abstract Cisplatin-induced ototoxicity is one of the major factors limiting cisplatin chemotherapy. Ototoxicity results from damage to outer hair cells (OHCs) and other regions cochlea. At cellular level, increases reactive oxygen species (ROS) leading cochlear inflammation apoptosis. Thus, ideal otoprotective drugs should target oxidative stress inflammatory mechanisms without interfering with cisplatin's chemotherapeutic efficacy. In this study, we show that epigallocatechin-3-gallate...
Previous studies have demonstrated the presence of cannabinoid 2 receptor (CB2R) in rat cochlea which was induced by cisplatin. In an organ Corti-derived cell culture model, it also shown that agonist CB2R protected these cells against cisplatin-induced apoptosis. current study, we determined distribution mouse and cochleae examined whether receptors provide protection hearing loss. a knock-in model expressing tagged with green fluorescent protein, show Corti, stria vascularis, spiral...
Abstract Capsaicin, the spicy component of hot chili peppers activates TRPV1 pain receptors, and causes rapid desensitization. Capsaicin also ameliorates cisplatin-induced nephrotoxicity. Cisplatin, a commonly used anti-neoplastic agent for solid tumors significant hearing loss, nephrotoxicity peripheral neuropathy. Upregulation cochlear expression is related to cisplatin-mediated ototoxicity. Here we report that direct activation by localized trans-tympanic (TT) or oral administration...
Abstract Cochlear hair cells can be killed by loud noises, ototoxic drugs, and natural aging. Once lost, mammalian do not naturally regenerate, leading to permanent hearing loss. Since the cochlea lacks any intrinsic ability genetic reprogramming of cochlear supporting that lie adjacent is a potential option for restoration therapies. We targeted with three cell transcription factors: Atoh1, or Atoh1 + Gfi1, Gfi1 Pou4f3 found 1- 2-factor sufficient reprogram adult into cells. However,...
The opportunistic fungal pathogen Candida albicans has the ability to exploit diverse host environments and can either reside commensally or cause disease. In order adapt its new environment it must respond physical conditions, nutrient sources, immune response. This requires co-regulation of multiple signalling networks. 14-3-3 family proteins is highly conserved in all eukaryotic species. These regulate pathways involved cell survival, cycle, differentiation, effect their functions via...
During development, planes of cells give rise to complex tissues and organs. The proper functioning these is critically dependent on inter- intra-cellular spatial orientation, a feature known as planar cell polarity (PCP). To study the genetic environmental factors affecting investigators must often manually measure orientations, which time-consuming endeavor.
Introdution: During development, planes of cells give rise to complex tissues and organs. The proper functioning these is critically dependent on inter- intra-cellular spatial orientation, a feature known as planar cell polarity (PCP). To study the genetic environmental factors affecting polarity, investigators must often manually measure orientations, which time-consuming endeavor. automate counting data collection we developed Fiji/ImageJ plug-in called PCP Auto Count (PCPA). Methods: PCPA...
Elongin C is a highly conserved, low molecular weight protein found in variety of multiprotein complexes human, rat, fly, worm, and yeast cells. Among the best characterized these mammalian E3 ligase that targets proteins for ubiquitination subsequent degradation by 26 S proteasome. Despite its crucial role as component such ligases other complexes, specific function unknown. In yeast, non-essential gene there no obvious phenotype associated with absence. We previously reported Saccharomyces...
Plants of the genus Cannabis have been used by humans for millennia a variety purposes. Perhaps most notable is use certain strains their psychoactive effects. More recently, several biologically active molecules within plants these strains, called phytocannabinoids or simply cannabinoids, identified. Furthermore, human cells, endogenous endocannabinoids, as well receptors and secondary messengers that give rise to neuromodulatory effects, also characterized. This endocannabinoid system...
Abstract Objectives The pervasiveness of hearing loss and the development new potential therapeutic approaches have led to increased animal studies inner ear. However, translational relevance such depends upon verification protein localization data in human samples. Cadavers used for anatomical education provide a research resource, but are limiting due difficulties accessing sensory tissues from dense temporal bones. This study seeks reduce often months‐long process decalcification improve...
Background: Globally, neonatal septicaemia is one of the common causes death making it a grave problem. Timely diagnosis and adequate treatment crucial for managing this burning issue.Aims Objectives: Early detection along with its infective etiological agent(s) assessment antimicrobial sensitivity pattern.Materials Methods: After getting approval from institutional ethics committee, an institution based observational, epidemiological study was conducted in ninety two neonates, clinically...
Abstract We have previously described Tapestry Pooling, a scheme to enhance the capacity of RT-qPCR testing, and provided experimental evidence with spiked synthetic RNA show that it can help scale testing restart economy. Here we report on validation studies Covid19 patient samples for Pooling prevalence in range 1% 2%. pooled extracted from were tested Covid19, sending each sample three pools. Following different pooling schemes, pipetted 320 into 48 pools pool size 20 at rate 1.6%, 500 60...
ABSTRACT Ribosomes, the molecular machines that are central to protein synthesis, have gradually been gaining prominence for their regulatory role in translation. Eukaryotic cytosolic ribosomes typically larger than bacterial ones, partly due multi-nucleotide insertions at specific conserved positions ribosomal RNAs (rRNAs). Such called expansion segments (ESs) present primarily on surface, with translation and its regulation remaining under-explored. One such ES large subunit (LSU) is...
Abstract Background: Data from this pre-clinical model suggests that the LAR TNBC subtype is unique and may respond to novel hormonal agents such as bicalutamide or abiraterone. The represents 11 % of all (which represent 20% breast cancers). Thus, positive studies abiraterone could potentially extend application an additional 2% cancer patients, who be treated in metastatic, neoadjuvant, adjuvant setting (for five years) with maintenance therapy. We, therefore, conducted on cell line,...
ABSTRACT The transcription factor GATA3 plays a critical role in the development of neurons and sensory epithelia inner ear. In mouse cochleae, is downregulated certain supporting cells (SCs) type I spiral ganglion (SGNs) after development. This reduction SCs severely limits Atoh1 -induced hair cell (HC) regeneration suggests that similar downregulation human cochleae may be limiting for regenerative therapies. However, it unknown whether similarly or differentially regulated primates versus...
A method for the quantitative estimation of gonadotropin inhibiting material (GIM) in human urine is standardised using ovarian ascorbic acid depletion test, results being expressed terms and HCG units. dose-response relationship with GIM established. The reliability assay demonstrated. Unlike other methods this able to express precisely quantity present urine. Preliminary data on values normal subjects as well patients endocrine disorders are presented.
Curvilinear Local Grid Refinement S. Ghosh Arco Exploration & Production Technology Search for other works by this author on: This Site Google Scholar Paper presented at the European Petroleum Conference, The Hague, Netherlands, October 1998. Number: SPE-50633-MS https://doi.org/10.2118/50633-MS Published: 20 1998 Cite View Citation Add to Manager Share Icon Twitter LinkedIn Get Permissions Ghosh, "Curvilinear Refinement." doi: Download citation file: Ris (Zotero) Reference EasyBib Bookends...
Studies have been made on the excretion of T3, T4 and cortisol in early morning urine specimens (EMU) ten male Indian sea level dwellers at a moderate altitude (2200 m) high (3658 m). EMU were markedly elevated comparison with values. The these parameters was also increased latitude their respective Plasma TSH remained unchanged base data. On return subjects from to conditions, studied urinary reverted original Key words: AltitudestressurinarycortisolT3 plasmahumans