A5054452522- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Spaceflight effects on biology
- RNA modifications and cancer
- Health and Medical Research Impacts
- Medical and Biological Ozone Research
- Career Development and Diversity
- Ubiquitin and proteasome pathways
- Space Exploration and Technology
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- Biomedical and Engineering Education
- Antifungal resistance and susceptibility
- Mass Spectrometry Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Doctoral Education Challenges and Solutions
- Advanced Proteomics Techniques and Applications
- Plant Virus Research Studies
- Reproductive Biology and Fertility
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- Mentoring and Academic Development
- Contemporary and Historical Greek Studies
- Bacteriophages and microbial interactions
Boston University
2011-2021
Marine Biological Laboratory
2020
Pennsylvania State University
2018
Albert Einstein College of Medicine
2018
Thomas Jefferson University
2018
Jefferson College of Health Sciences
2018
Jefferson College
2018
University of Florida Health
2018
Montana State University
2004-2013
Tulane University
1998-2003
Summary A sequence of 5987 nucleotides is reported for the RNA potato leafroll luteovirus (PLRV). The contains six large open reading frames, and non-coding regions 174 at 5′ end, 141 3′ end 197 between two blocks coding sequences. region encodes polypeptides 28000 (28K) 70K which overlap in different frames circumstantial evidence suggests that third frame block translated by frameshift readthrough near polypeptide to give a 118K polypeptide. C-terminal part protein consensus RNA-dependent...
The pBEVY (bi-directional expression vectors for yeast) plasmids were designed with constitutive and galactose-induced bi-directional promoters to direct the of multiple proteins in Saccharomyces cerevisiae . Using human estrogen receptor as a test gene, relatively balanced levels from each side promoter observed. Expression functional heterodimeric transcription factor composed aryl hydrocarbon (Ahr) nuclear translocator (Arnt) was accomplished using single plasmid. Previous studies suggest...
Ribosomal protein synthesis ceases upon maturation of Xenopus oocytes. We find that this cessation results from the dissociation ribosomal mRNAs polysomes and is accompanied by deadenylation these transcripts. A synthetic mRNA encoding L1, microinjected into stage VI oocytes, deadenylated released maturation. Our indicate sequences located within 387 bp 3' terminus L1 direct both polysomal release mRNA. The proper translational regulation an exogenous in oocytes provides a basis for...
This study presents the first global transcriptional profiling and phenotypic characterization of major human opportunistic fungal pathogen, Candida albicans, grown in spaceflight conditions. Microarray analysis revealed that C. albicans subjected to short-term culture differentially regulated 452 genes compared synchronous ground controls, which represented 8.3% analyzed ORFs. Spaceflight-cultured albicans-induced involved cell aggregation (similar flocculation), was validated by...
ABSTRACT Only limited information is available concerning the effects of low-shear modeled microgravity (LSMMG) on cell function and morphology. We examined behavior Saccharomyces cerevisiae grown in a high-aspect-ratio vessel, which simulates conditions encountered spaceflight. With exception shortened lag phase (90 min less than controls; P < 0.05), yeast cells under LSMMG did not differ growth rate, size, shape, or viability from controls but establishment polarity as exhibited by...
Candida albicans is an opportunistic fungal pathogen responsible for a variety of cutaneous and systemic human infections. Virulence C. increases upon exposure to some environmental stresses; therefore, we explored phenotypic responses following the stress low-shear modeled microgravity. Upon long-term (12-day) microgravity, transitioned from yeast filamentous forms at higher rate than observed under control conditions. Consistently, genes associated with cellular morphology were...
PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and nonprofit organizations. Hence, best practices for training future biomedical of national concern. Complementing coursework laboratory research training, many institutions now offer professional enables career exploration develops a broad set skills critical various paths. The National Institutes Health (NIH) funded academic design innovative...
The sequences directing formation of mRNA 3' ends in Saccharomyces cerevisiae are not well defined. This is contrast to the situation higher eukaryotes which sequence AAUAAA known be crucial proper 3'-end formation. hexanucleotide found upstream poly(A) site some but all yeast genes. One these gene coding for alcohol dehydrogenase, ADH2. Deletion or a double point mutation has only small effect on efficiency reaction, and mammalian system, it most likely operating as major processing signal...
Abstract Knowledge of simulated microgravity (SMG)-induced changes in the pathogenicity microorganisms is important for success long-term spaceflight. In a previous study using high aspect ratio vessel bioreactor, we showed that yeast species Saccharomyces cerevisiae underwent significant phenotypic response when grown modeled microgravity, which was reflected analysis gene expression profiles. this study, establish Candida albicans responds to SMG similar fashion, demonstrating there...
The Broadening Experiences in Scientific Training (BEST) program is an NIH-funded effort testing the impact of career development interventions (e.g. internships, workshops, classes) on biomedical trainees (graduate students and postdoctoral fellows). BEST Programs seek to increase trainees' knowledge, skills confidence explore pursue expanded options, as well training new that enable multiple pathways. Faculty mentors are vital a trainee's professional development, but data about how...
Little is known about the transcriptional events which occur downstream of polyadenylation sites. Although site a gene can be easily identified, it has been difficult to determine transcription termination in vivo because rapid processing pre-mRNAs. Using an vitro approach, we have shown that sequences from 3' ends two different Saccharomyces cerevisiae genes, ADH2 and GAL7, direct and/or polymerase pausing yeast nuclear extracts. In case sequence, RNA synthesized approximately 50 150...
Abstract The low-shear microgravity environment, modeled by rotating suspension culture bioreactors called high aspect ratio vessels (HARVs), allows investigation in ground-based studies of the effects on eukaryotic cells and provides insights into impact space flight cellular physiology. We have previously demonstrated that (LSMMG) causes significant phenotypic changes a select group Saccharomyces cerevisiae genes associated with establishment cell polarity, bipolar budding, separation....
A role of the FLO11 in Saccharomyces cerevisiae biofilm development a flow cell system was examined. We carried out an ectopic expression wild type (wt) BY4741 strain that has low levels endogenous transcript. In contrast to nonadhesive wt, overexpression (BY4741 FLO11(+)) readily adhered both liquid-hydrophobic and liquid-hydrophilic solid interfaces able grow as monolayer system. Cellular features associated with were examined found be consistent previous studies conducted different...
This study utilizes Saccharomyces cerevisiae to genetic responses suspension culture. The culture system used in this is the high-aspect-ratio vessel, one type of rotating wall that provides a high rate gas exchange necessary for rapidly dividing cells. Cells were grown and DNA microarray metabolic analyses determine resulting changes yeast gene expression. A significant number genes found be up- or downregulated by at least twofold as result rotational growth. By using Gibbs promoter...
The specific RNA-protein interactions responsible for the production of mature 3' ends eukaryotic mRNAs are not well understood. Sequence elements at yeast genes have been identified that specify position poly(A) site and efficiency polyadenylation. To provide additional insights into interaction between important sequences direct 3'-end formation in vivo nuclear proteins, we utilized gel mobility shift assays UV-crosslinking studies. data indicate a protein, with an apparent molecular...
Little is known about the transcriptional events which occur downstream of polyadenylation sites. Although site a gene can be easily identified, it has been difficult to determine transcription termination in vivo because rapid processing pre-mRNAs. Using an vitro approach, we have shown that sequences from 3' ends two different Saccharomyces cerevisiae genes, ADH2 and GAL7, direct and/or polymerase pausing yeast nuclear extracts. In case sequence, RNA synthesized approximately 50 150...
The sequences directing formation of mRNA 3' ends in Saccharomyces cerevisiae are not well defined. This is contrast to the situation higher eukaryotes which sequence AAUAAA known be crucial proper 3'-end formation. hexanucleotide found upstream poly(A) site some but all yeast genes. One these gene coding for alcohol dehydrogenase, ADH2. Deletion or a double point mutation has only small effect on efficiency reaction, and mammalian system, it most likely operating as major processing signal...
Transcription termination and 3′ end formation are essential processes necessary for gene expression. However, the specific mechanisms responsible these events remain elusive. A screen designed to identify trans-acting factors involved in Saccharomyces cerevisiae identified a temperature-sensitive mutant that displayed phenotypes consistent with role transcription termination. The complementing was as GRS1, which encodes S. glycyl-tRNA synthetase. This result, although unusual, is not...
Elongin C is a highly conserved, low molecular weight protein found in variety of multiprotein complexes human, rat, fly, worm, and yeast cells. Among the best characterized these mammalian E3 ligase that targets proteins for ubiquitination subsequent degradation by 26 S proteasome. Despite its crucial role as component such ligases other complexes, specific function unknown. In yeast, non-essential gene there no obvious phenotype associated with absence. We previously reported Saccharomyces...
The Division of Graduate Medical Sciences at the Boston University School Medicine houses numerous dynamic graduate programs. Doctoral students began their studies with laboratory rotations and classroom training in a variety fundamental disciplines. Importantly, 15 unique pathways admission to these doctoral programs, there were also curricula. Departments programs offered courses independently, participated curricula that overlapping combinations courses. This system created not...
Abstract 3′-end formation is a complex and incompletely understood process involving both cis-acting trans-acting factors. As part of an effort to examine the mechanisms transcription termination by RNA polymerase II, mutant hunt for strains defective in was conducted. Following random mutagenesis, temperature-sensitive strain exhibiting several phenotypes consistent with role isolated. First, readthrough terminator increases significantly strain. Accordingly, analysis indicates decrease...