A5043212035- CAR-T cell therapy research
- SARS-CoV-2 and COVID-19 Research
- Health and Medical Research Impacts
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Respiratory viral infections research
- Pluripotent Stem Cells Research
- Viral Infections and Outbreaks Research
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Biomedical Ethics and Regulation
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Career Development and Diversity
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Hepatitis C virus research
- Virus-based gene therapy research
- Glioma Diagnosis and Treatment
- Animal Genetics and Reproduction
Texas Biomedical Research Institute
2023
University of Rochester
2020-2021
University of Rochester Medical Center
2019
Harvard University
2010-2014
University of Wisconsin–Madison
2014
Boston Children's Hospital
2014
University of Pennsylvania
2013
Cancer Research Institute
2013
National University of Singapore
2013
Quantitative BioSciences
2012
Establishing relationships among specific quality traits is important if significant progress toward developing improved pork to be realized. As part of a study examine the individual effects genes on meat in pigs, three-generation resource family was developed. Two Berkshire sires and nine Yorkshire dams were used produce F1 litters. Sixty-five matings made from litters four sets F2 offspring, for total 525 animals study. These slaughtered at commercial facility upon reaching approximately...
ABSTRACT Several respiratory viruses, including influenza virus and severe acute syndrome coronavirus (SARS-CoV), produce more disease in the elderly, yet molecular mechanisms governing age-related susceptibility remain poorly studied. Advanced age was significantly associated with increased SARS-related deaths, primarily due to onset of early- late-stage distress (ARDS) pulmonary fibrosis. Infection aged, but not young, mice recombinant viruses bearing spike glycoproteins derived from early...
Recent outbreaks of avian influenza in humans have stressed the need for an improved nonhuman primate model pathogenesis. In order to further develop a macaque model, we expanded our previous vivo genomics experiments with virus-infected macaques by focusing on innate immune response at day 2 postinoculation and gene expression affected lung tissue viral genetic material present. Finally, sought identify signature genes early infection whole blood. For these purposes, infected six pigtailed...
ABSTRACT The development of a reproducible model system for the study hepatitis C virus (HCV) infection has potential to significantly enhance virus-host interactions and provide future direction modeling pathogenesis HCV. While there are studies describing global gene expression changes associated with HCV infection, in proteome have not been characterized. We report first large-scale analysis highly permissive Huh-7.5 cell line containing full-length replicon. detected >4,200 proteins...
The pathogenesis of severe acute respiratory syndrome coronavirus (SARS-CoV) is likely mediated by disproportional immune responses and the ability virus to circumvent innate immunity. Using functional genomics, we analyzed early host SARS-CoV infection in lungs adolescent cynomolgus macaques (Macaca fascicularis) that show lung pathology similar observed human adults with SARS. Analysis gene signatures revealed induction a strong response characterized stimulation various cytokine chemokine...
PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and nonprofit organizations. Hence, best practices for training future biomedical of national concern. Complementing coursework laboratory research training, many institutions now offer professional enables career exploration develops a broad set skills critical various paths. The National Institutes Health (NIH) funded academic design innovative...
ABSTRACT Herpes simplex virus type 1 (HSV-1) mutants lacking the ICP34.5 gene are severely attenuated in mouse models and have a significant growth defect confluent embryo fibroblasts. Previously, was demonstrated to crucial role evading innate immune response infection by mediating dephosphorylation of eIF2α, translation initiation factor phosphorylated PKR during antiviral response. To further understand evasion response, we used transcriptional profiling examine host cell expression both...
ABSTRACT The relationship between immunosenescence and the host response to virus infection is poorly understood at molecular level. Two different patterns of pulmonary responses were observed when gene expression profiles from severe acute respiratory syndrome coronavirus (SARS-CoV)-infected young mice that show minimal disease compared those SARS-CoV-infected aged develop pneumonitis. In mice, genes related cellular development, cell growth, cycle downregulated during peak viral...
The Broadening Experiences in Scientific Training (BEST) program is an NIH-funded effort testing the impact of career development interventions (e.g. internships, workshops, classes) on biomedical trainees (graduate students and postdoctoral fellows). BEST Programs seek to increase trainees' knowledge, skills confidence explore pursue expanded options, as well training new that enable multiple pathways. Faculty mentors are vital a trainee's professional development, but data about how...
These studies attempt to understand more fully the host response and pathogenesis associated with severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) by monitoring gene expression using formalinfixed paraffin-embedded (FFPE) pulmonary autopsy tissues. tissues were from patients in different hospitals Singapore who diagnosed various microbial infections, including SARS-CoV, that caused distress (ARDS). Global patterns showed limited correlation between end-stage ARDS initiating...
One side of a triangular molecular tile has been successfully synthesized. The synthesized peptide contains two cysteines (Cys), glutamic acids (Glu), and p-aminomethylbenzoyl-p-benzoic acid (AMBBA). anchor the to gold surface by formation gold−thiolate bonds. negatively charged should assist in binding positively proteins peptide. rigid AMBBA provides benzophenone group for photochemically tethering also acts as corner triangle. Electron spectroscopy chemical analysis (ESCA) near-edge X-ray...
Viruses employ different strategies to circumvent the antiviral actions of innate immune response. SARS coronavirus (SARS-CoV), a virus that causes severe lung damage, encodes an array proteins able inhibit induction and signaling type-I interferons. However, recent studies have demonstrated interferons are produced during SARS-CoV infection in humans macaques. Furthermore, nuclear translocation activated STAT1 range interferon-stimulated genes could be lungs SARS-CoV-infected In line with...
Protein layers are deposited on the surface of implanted biomaterials. Better understanding interaction between protein and biological system would lead to development future biomaterials with superior biocompatibilities. Well-organized biorecognizable surfaces can be formed various template molecules that provide an appropriate spacing for attachment recognition groups modified surface. Silane coupling reagents, porphyrin thiols, cyclic peptides being used as templates introduce nano-scale...
Twelve different mating types among the Hampshire and Landrace breeds were used to determine direct, maternal, heterosis, recombination effects for performance carcass traits. Mating two purebred, F1, F2, F3, four backcross. Carcass data collected on 238 barrows 262 gilts over replications. Traits measured length (LENG), 10th rib off midline backfat (BF10), longissimus muscle area (LMA), dressing percentage (DRS%). Average (AVBF) was calculated as mean of three fat depths opposite first rib,...
Abstract PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and non-profit organizations. Hence, best practices for training future biomedical of national concern. Complementing coursework laboratory research training, many institutions now offer professional enables career exploration develops a broad set skills critical various paths. The National Institutes Health funded academic design innovative...
Abstract Summary We have developed a web-based tool, CoDe (Codon Deoptimization) that deoptimizes genetic sequences based on different codon usage bias, ultimately reducing expression of the corresponding protein. The tool could also deoptimize sequence for specific region and/or selected amino acid(s). Moreover, can highlight sites targeted by restriction enzymes in wild-type and codon-deoptimized sequences. Importantly, our has user-friendly interface with flexible options to download...