A5032674188- Retinoids in leukemia and cellular processes
- Cancer-related Molecular Pathways
- Ocular Oncology and Treatments
- Health and Medical Research Impacts
- Ubiquitin and proteasome pathways
- Career Development and Diversity
- Doctoral Education Challenges and Solutions
- Estrogen and related hormone effects
- T-cell and Retrovirus Studies
- Acute Myeloid Leukemia Research
- Diversity and Career in Medicine
- Higher Education Practises and Engagement
- Cancer, Hypoxia, and Metabolism
- Herpesvirus Infections and Treatments
- Antioxidant Activity and Oxidative Stress
- interferon and immune responses
- Polyomavirus and related diseases
- Histone Deacetylase Inhibitors Research
- Higher Education and Employability
- Service-Learning and Community Engagement
- Multiple Myeloma Research and Treatments
- Human Resource Development and Performance Evaluation
- Chemokine receptors and signaling
- Melanoma and MAPK Pathways
- Monoclonal and Polyclonal Antibodies Research
Cornell University
1999-2024
NCCR Chemical Biology - Visualisation and Control of Biological Processes Using Chemistry
2011
University of Geneva
2011
In-Q-Tel
2001
Tokyo University of Agriculture
1994
Georgetown University
1992
National Institutes of Health
1987
National Cancer Institute
1987
A novel human B-lymphotropic virus (HBLV) was isolated from the peripheral blood leukocytes of six individuals: two HTLV-III seropositive patients United States (one with AIDS-related lymphoma and one dermatopathic lymphadenopathy), three seronegative angioimmunoblastic lymphadenopathy, cutaneous T-cell lymphoma, immunoblastic lymphoma), patient acute lymphocytic leukemia Jamaica. All isolates were closely related by antigenic analysis, sera all virus-positive reacted immunologically each...
Experiential learning is an effective educational tool across many academic disciplines, including career development. Nine different institutions bridged by the National Institutes of Health Broadening Experiences in Scientific Training Consortium compared their experiments rethinking and expanding training predoctoral graduate students postdoctoral scholars biomedical sciences to include experiential opportunities. In this article, we provide overview four types approaches our offer...
PhD-trained scientists are essential contributors to the workforce in diverse employment sectors that include academia, industry, government, and nonprofit organizations. Hence, best practices for training future biomedical of national concern. Complementing coursework laboratory research training, many institutions now offer professional enables career exploration develops a broad set skills critical various paths. The National Institutes Health (NIH) funded academic design innovative...
The present study examines racial, ethnic, and gender disparities in career self-efficacy amongst 6077 US citizens naturalized graduate postdoctoral trainees. Respondents from biomedical fields completed surveys administered by the National Institutes of Health Broadening Experiences Scientific Training (NIH BEST) programs across 17 institutional sites. Graduate demographic survey response data were examined to evaluate impact intersectional identities on trainee self-efficacy. hypothesized...
The infection of a number new and established B-cell lines by human T-cell lymphotropic virus III (HTLV-III) was investigated. B lymphocytes differed in their expression T4 antigens detected specific monoclonal antibodies (MAbs) the presence Epstein Barr (EBV)-DNA or antigens. EBV genome only requirement for B-lymphocytes HTLV-III, although its did not ensure infection. Two antigen-positive lines, lacking early (EA) viral capsid (VCA), could be productively infected with no induction known...
This study examines the intersectional role of citizenship and gender with career self-efficacy amongst 10,803 doctoral postdoctoral trainees in US universities. These biomedical completed surveys administered by 17 institutions that participated National Institutes Health Broadening Experiences Scientific Training (NIH BEST) Programs. Findings indicate non-citizen is significantly lower than citizen trainees. While efficacy was observed women compared men, it even for female Results suggest...
Abstract Retinoic acid‐induced expression of the CD38 ectoenzyme receptor in HL‐60 human myeloblastic leukemia cells is regulated by RARα and RXR, enhanced or prevented cell differentiation depending on level per cell. activation caused expression, as did RXR but not effectively. Inhibition MAPK signaling through MEK inhibition diminished induced both RARs RXRs. Expression retinoic myeloid G0 cycle arrest, at higher levels, was blocked acid a loss viability instead. In case...
The cell cycle phase-specific phosphorylation status of the RB protein (retinoblastoma tumor suppressor gene product) during an elicited cellular program G0 arrest and differentiation was characterized. state is presumed to be important determinant proliferation or arrest. phase specificity dephosphorylation HL-60 human leukemia determined using DNA-based fluorescence-activated sorting Western analysis. in proliferating G1 cells phosphorylated, but at a relatively low level. extent increased...
Abstract The dependence of induced myelomonocytic cell differentiation, and regulation the RB tumor suppressor gene during this process, on c‐fms product, CSF‐1 lymphokine receptor, was determined in HL‐60 promyelocytic leukemia cells. Adding a monoclonal antibody with specificity for product to cells treated various inducers or macrophage including retinoic acid 1,25‐dihydroxy vitamin D3, inhibited rate differentiation. During period inducer treatment usually preceding onset longer periods...
Radicicol, an inhibitor of src or src-like kinases, causes hypophosphorylation and down-regulation the RB retinoblastoma tumor suppressor protein in HL-60 human promyelocytic leukemia cells. Both these changes typically are associated with myeloid monocytic differentiation cells induced by retinoic acid 1,25-dihydroxy vitamin D3. When added either inducer, radicicol caused to be accelerated. By itself a transient inhibition G1 S transit, but did not cause phenotypic conversion. The...
The effect of the CSF-1 receptor, cFMS, on phosphorylation retinoblastoma (RB) tumor suppressor protein and cell cycle differentiation was analyzed in a cultured promyelocytic leukemia capable induced myelomonocytic differentiation. A series cFMS-transfected HL-60 sublines with progressively higher surface FMS expression derived by flow cytometric sorting. Overexpression increased duration cycle, prolonging all phases especially S phase, which doubled. generation times occurred without any...
Expression of the RB retinoblastoma tumor suppressor gene product is regulated early during stimulation normal human peripheral blood lymphocytes, suggesting a regulatory role for amount this protein in mitogenesis cells. When lymphocytes were mitogenically stimulated with pokeweed mitogen, bivariate flow cytometric measurements cellular DNA and content showed an decrease per cell, anteceding onset S phase. A subsequent increase cell occurred proliferation. Thus relative to total mass...
Chimpanzee secretory immunoglobulin A (SIgA) was separated into two fractions by chromatography using the terminal galactose‐binding lectin Jacalin. The SIgA fraction bound Jacalin cleaved Haemophilus influenzae IgA1 protease, whereas nonbinding not cleaved. It is proposed that these represent and IgA2 subclasses because presence or absence of galactose‐terminal oligosaccharides (Jacalin binding) susceptibility resistance to protease are properties define human subclasses.
In HL-60 human myeloblastic leukemia cells, retinoic acid is known to cause cFMS, RAF, MEK, and ERK2 dependent myeloid cell differentiation G0 arrest associated with RB tumor suppressor protein hypophosphorylation, implicating receptor tyrosine kinase signal transduction in propelling these acid-induced cellular effects. Furthermore, ectopic expression of polyoma middle T antigen, which activates similar early molecules as PDGF class receptors such accelerates To determine if this depends on...
Abstract To examine the potential coupling between inducible cellular changes in RB (retinoblastoma) tumor suppressor protein phosphorylation and ability to G0 growth arrest differentiate, HL‐60 promyelocytic leukemia cells were cultured incremental sodium butyrate (NaB) concentrations thereby made resistant inhibitory effects of butyrate, which normally induces monocytic differentiation wild type cells. The also unable differentiate response NaB, indicating that a regulatory function...