A5000896186- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Blood properties and coagulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neonatal Health and Biochemistry
- Bone and Joint Diseases
- Erythropoietin and Anemia Treatment
- Virus-based gene therapy research
- Pharmacological Effects and Toxicity Studies
- Hemoglobin structure and function
- T-cell and B-cell Immunology
- Prenatal Screening and Diagnostics
- Hematological disorders and diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Parvovirus B19 Infection Studies
- Genital Health and Disease
- Exercise and Physiological Responses
- Heart Rate Variability and Autonomic Control
- Hip disorders and treatments
- Pneumocystis jirovecii pneumonia detection and treatment
- Endoplasmic Reticulum Stress and Disease
- Neurological and metabolic disorders
- Viral Infectious Diseases and Gene Expression in Insects
Centre Hospitalier Universitaire Pointe-à-Pitre
2016-2025
Biologie Intégrée du Globule Rouge
2017-2024
Inserm
2007-2024
Université Paris Cité
2014-2024
Université des Antilles
2005-2022
Laboratory of Excellence GR-Ex
2013-2022
Physiopathologie et Epidémiologie des Maladies Respiratoires
2008-2019
University of French Guiana
2019
Sorbonne Université
2015
Sorbonne Paris Cité
2014
Recent evidence suggests that red blood cell aggregation and the ratio of hematocrit to viscosity (HVR), an index oxygen transport potential blood, might considerably modulate flow dynamics in microcirculation. It thus seems likely these factors could play a role sickle disease.We compared characteristics, HVR at different shear rates between anemia hemoglobin C disease (SCC) patients, trait carriers (AS) control individuals (AA).Blood determined high rate was lower (n=21) than AA (n=52), AS...
Although pulmonary hypertension, leg ulcers, priapism, stroke and glomerulopathy in sickle cell anaemia (SCA) result from the adverse effects of chronic haemolysis on vascular function (haemolytic phenotype), osteonecrosis, acute chest syndrome painful vaso-occlusive crises are caused by abnormal adhesion increased blood viscosity (viscosity-vaso-occlusion phenotype). However, this model with two sub-phenotypes does not take into account haemorheological dimension. We tested relationships...
Patients with sickle cell disease hospitalised for acute chest syndrome (ACS) are at high risk of in situ pulmonary microthrombosis. We evaluated whether therapeutic anticoagulation could shorten ACS duration. TASC is a randomized, controlled, double-blind trial conducted 12 French hospitals (December 2016-April 2021) adult patients no initial thrombosis on computerised tomography angiogram. randomised 172 (1:1) to receive either prophylactic or doses low-molecular-weight tinzaparin 7 days....
Background Little is known about the effects of blood rheology on occurrence acute chest syndrome and painful vaso-occlusive crises in children with sickle cell anemia hemoglobin SC disease.Design Methods To address this issue, steady-state hemorheological profiles (blood viscosity, red deformability, aggregation properties) hematologic parameters were assessed 44 49 disease (8-16 years old) followed since birth. Clinical charts retrospectively reviewed to determine prior or episodes, rates...
Abstract Acute splenic sequestration crisis (ASSC) is a potentially life‐threatening complication of sickle cell disease (SCD), typically occurring in young patients under 5 years age, with median age at first episode less than 2 years. Because beneficial effect hydroxyurea (HU) on spleen perfusion and function has been suspected, we hypothesized that HU treatment might be associated later onset ASSC SCD. To investigate this hypothesis, analyzed data from the ESCORT‐HU study large cohort SCD...
As an approach to cell targeting by retroviruses, the lack of which constitutes one major limitation retroviral vector technology, we engineered Moloney murine leukemia virus ecotropic envelope glycoprotein. When inserted between amino acids 6 and 7 latter, a single-chain antibody fragment (ScFv) specific for human histocompatibility complex class I molecules was shown be able redefine tropism virus-derived particles allowing infection I-positive cells. At variance with other recently...
Leg ulcer is a disabling complication in patients with sickle cell anemia (SCA) but the exact pathophysiological mechanisms are unknown. The aim of this study was to identify hematological and hemorheological alterations associated recurrent leg ulcers. Sixty-two SCA who never experienced ulcers (ULC-) 13 positive history (ULC+)--with no at time study--were recruited. All were steady state condition. Blood sampled perform hematological, biochemical (hemolytic markers) analyses (blood...
Chronic hemolysis, enhanced oxidative stress and decreased nitric oxide (NO) bioavailability promote vasculopathy in sickle cell anemia (SCA). Oxidative NO are known to modulate eryptosis healthy red blood cells (RBCs), however their role SCA impact on the genesis of RBC-derived microparticles (RBC-MPs) remains poorly described. RBC-MPs could play a vascular dysfunction SCA. The aims this study were evaluate roles release, determine whether involved Markers stress, plasma concentration...
Sickle cell disease (SCD) is a severe hematological disorder. The most common acute complication of SCD vaso-occlusive crisis (VOC), but systemic potentially involving all organs. prevalence estimates rely mostly on extrapolations from incidence-based newborn screening programs, although recent improvements in survival may have led to an increase prevalence, and immigration could account for substantial number prevalent patients Europe. primary objective this study was estimate France.A...
L-glutamine has been shown to play an important role in the regulation of oxidative stress which is one key contributors pathophysiology sickle cell disease (SCD). In a Phase 3 clinical trial, demonstrated significant reduction SCD-related complications including vaso-occlusive crises (VOCs), hospitalizations, and acute chest syndrome (ACS) compared placebo patients with SCD.The primary objective was confirm efficacy (Endari®) therapy pediatric adult SCD at follow-up time points 24, 48 72...
Using streptavidin-bound antibodies specific for both viral and cell membrane epitopes, we have reported previously that human cells may be infected by murine ecotropic retroviruses through an interaction with major histocompatibility complex class I II antigens, thus demonstrated targeting recombinant is feasible. We report here (i) growth factor or hormone receptors, such as those epidermal (EGF) insulin, can also mediate infection of cells; (ii) a biotinylated cytokine substitute the...
The hematocrit-to-viscosity ratio (HVR) has been widely used an estimate of red blood cell (RBC) oxygen transport effectiveness into the microvasculature or as delivery index. However, no study investigated possibility HVR to truly reflect RBC be We measured viscosity at high shear rate (225 s(-1)), hematocrit, HVR, well microvascular oxyhemoglobin saturation (TOI; tissue index) by spatial resolved near-infrared spectroscopy (NIRS) cerebral and muscle levels in three population known have...
While chronic hemolysis has been suspected to be involved in the development of glomerulopathy patients with sickle cell anemia (SCA), no study focused on implications blood rheology. Ninety-six adults SCA at steady state were included present cross-sectional study. Three categories defined: normo-albuminuria (NORMO, n = 41), micro-albuminuria (MICRO, 23) and macro-albuminuria (MACRO, 32). Blood was sampled measure hematological hemorheological parameters, genomic DNA extraction performed...
How to assess mental load remains a recurrent question. We aimed explore whether slight differences in real-world driving task demands could be discriminated by electrodermal response (EDR). A sample of 33 participants was observed under five conditions: controlled braking from 50 30 km/h, 80 0 and single unexpected emergency event km/h. The likelihood EDR and, whenever present, its duration were both correlated with workload as represented the deceleration demand. higher base travel speed...
Blood rheology plays a key role in the pathophysiology of sickle cell anaemia (SS) and haemoglobin C disease (SC), but its evolution over lifespan is unknown.Blood viscosity, red blood (RBC) deformability aggregation, foetal (HbF) haematocrit were measured 114 healthy individuals (AA), 267 SS (161 children + 106 adults) 138 SC (74 64 patients.Our results showed that 1) RBC at maximal value during early years life populations, mainly because HbF level also peak, 2) childhood adulthood,...
Summary Human red blood cells ( RBC ) express an active and functional endothelial‐like nitric oxide NO synthase RBC‐NOS ). We report studies on activity in sickle cell anaemia SCA ), a genetic disease characterized by decreased deformability vascular dysfunction. Total content was not significantly different patients compared to healthy controls; however, using phosphorylated ‐Ser 1177 as marker, activation higher consequence of the greater Akt (phosphorylated Akt‐Ser 473 The led levels...
Summary Painful vaso‐occlusive crisis, a hallmark of sickle cell anaemia, results from complex, incompletely understood mechanisms. Red blood ( RBC ) damage caused by continuous endogenous and exogenous oxidative stress may precipitate the occurrence crises. In order to gain insight into relevance in crisis occurrence, we prospectively compared expression levels various markers 32 adults with anaemia during steady‐state conditions. Compared condition, plasma free haem, advanced oxidation...
Abstract Background Sickle cell anaemia (SCA) is a severe hereditary haemoglobinopathy characterised by haemorheological abnormalities, which play role in the occurrence of several acute and chronic clinical complications. While β S ‐haplotypes alpha‐thalassaemia modulate SCA severity, their effects on blood rheology have been incompletely described. The aim this study was to test these genetic modifiers properties complication children with SCA. Procedure Steady‐state profile, biological...
Objectives To investigate the association between priapism in men with sickle cell anemia (SCA) and hemorheological hemolytical parameters. Materials Methods Fifty-eight SCA (median age: 38 years) were included; 28 who had experienced at least once during their life (priapism group) 30 never this complication (control group). Twenty-two patients treated hydroxycarbamide, 11 each group. All steady state time of inclusion. Hematological biochemical parameters obtained through routine...