A5002422164- Hemoglobinopathies and Related Disorders
- Blood properties and coagulation
- Iron Metabolism and Disorders
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Effects of Environmental Stressors on Livestock
- Heart Rate Variability and Autonomic Control
- Obstructive Sleep Apnea Research
- Neuroscience of respiration and sleep
- High Altitude and Hypoxia
- Bone and Joint Diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cardiovascular Health and Disease Prevention
- Cardiovascular and exercise physiology
- Hemoglobin structure and function
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Traumatic Brain Injury and Neurovascular Disturbances
- Meat and Animal Product Quality
- Adipose Tissue and Metabolism
- Healthcare Regulation
- Neurological and metabolic disorders
- Pulmonary Hypertension Research and Treatments
- Erythropoietin and Anemia Treatment
- Parvovirus B19 Infection Studies
- Climate Change and Health Impacts
Inserm
2012-2020
Centre Hospitalier Universitaire de Grenoble
2020
Université Grenoble Alpes
2017-2020
Université des Antilles
2012-2017
Laboratory of Excellence GR-Ex
2012-2017
Sorbonne Paris Cité
2012-2017
Université Paris Cité
2012-2017
University of Calgary
2015-2017
Centre Hospitalier Universitaire Pointe-à-Pitre
2013-2017
Biologie Intégrée du Globule Rouge
2017
Although pulmonary hypertension, leg ulcers, priapism, stroke and glomerulopathy in sickle cell anaemia (SCA) result from the adverse effects of chronic haemolysis on vascular function (haemolytic phenotype), osteonecrosis, acute chest syndrome painful vaso-occlusive crises are caused by abnormal adhesion increased blood viscosity (viscosity-vaso-occlusion phenotype). However, this model with two sub-phenotypes does not take into account haemorheological dimension. We tested relationships...
Background Little is known about the effects of blood rheology on occurrence acute chest syndrome and painful vaso-occlusive crises in children with sickle cell anemia hemoglobin SC disease.Design Methods To address this issue, steady-state hemorheological profiles (blood viscosity, red deformability, aggregation properties) hematologic parameters were assessed 44 49 disease (8-16 years old) followed since birth. Clinical charts retrospectively reviewed to determine prior or episodes, rates...
Sickle cell anaemia (SS) and sickle cell-haemoglobin C disease (SC) patients exhibit severe red blood (RBC) rheological alterations involved in the development of several complications. The contribution oxidative stress these haemorheological abnormalities is still unknown. We compared RBC reactive oxygen species (ROS) glutathione (GSH) content, profile SS (n = 11), SC 11) healthy subjects 12) at baseline after in-vitro treatment with t-butyl hydroperoxide (TBHP). showed: (i) higher ROS...
Leg ulcer is a disabling complication in patients with sickle cell anemia (SCA) but the exact pathophysiological mechanisms are unknown. The aim of this study was to identify hematological and hemorheological alterations associated recurrent leg ulcers. Sixty-two SCA who never experienced ulcers (ULC-) 13 positive history (ULC+)--with no at time study--were recruited. All were steady state condition. Blood sampled perform hematological, biochemical (hemolytic markers) analyses (blood...
The hematocrit-to-viscosity ratio (HVR) has been widely used an estimate of red blood cell (RBC) oxygen transport effectiveness into the microvasculature or as delivery index. However, no study investigated possibility HVR to truly reflect RBC be We measured viscosity at high shear rate (225 s(-1)), hematocrit, HVR, well microvascular oxyhemoglobin saturation (TOI; tissue index) by spatial resolved near-infrared spectroscopy (NIRS) cerebral and muscle levels in three population known have...
While chronic hemolysis has been suspected to be involved in the development of glomerulopathy patients with sickle cell anemia (SCA), no study focused on implications blood rheology. Ninety-six adults SCA at steady state were included present cross-sectional study. Three categories defined: normo-albuminuria (NORMO, n = 41), micro-albuminuria (MICRO, 23) and macro-albuminuria (MACRO, 32). Blood was sampled measure hematological hemorheological parameters, genomic DNA extraction performed...
Blood rheology plays a key role in the pathophysiology of sickle cell anaemia (SS) and haemoglobin C disease (SC), but its evolution over lifespan is unknown.Blood viscosity, red blood (RBC) deformability aggregation, foetal (HbF) haematocrit were measured 114 healthy individuals (AA), 267 SS (161 children + 106 adults) 138 SC (74 64 patients.Our results showed that 1) RBC at maximal value during early years life populations, mainly because HbF level also peak, 2) childhood adulthood,...
Key points Highlanders develop unique adaptative mechanisms to chronic hypoxic exposure, including substantial haemoglobin and haematocrit increases. However, a significant proportion of populations living permanently at high altitude maladaptive features known as mountain sickness (CMS). This study aimed assess the effects permanent life on clinical haemorheological parameters (blood viscosity red blood cell aggregation) compare dwellers from highest city in world according CMS severity....
Because of the metabolic changes induced by a physical activity, hemorheological properties patients with sickle cell anemia could be further impaired and increase risks for vaso-occlusive complications. However, few studies suggest that moderate activity beneficial rather than harmful in (SCA). definition what can considered as SCA is imprecise. The present study tested effects short incremental cycling exercise test conducted until first ventilatory threshold on different biomarkers....
We tested the effects of submaximal exercise on blood viscosity (η(b)), nitric oxide production (NO) and hemodynamics. Relationships between exercise-induced changes that occurred in these parameters were investigated. Nine subjects performed for 15 min at 105% first ventilatory threshold. Mean arterial pressure (MAP) cardiac output (Qc) measured, allowing determination systemic vascular resistance (SVR). Blood was sampled rest end exercise. The η(b) determined high shear rate used to...
Although it has been hypothesized that muscle metabolism and fatigability could be impaired in sickle cell patients, no study addressed this issue.We compared function (muscle microvascular oxygenation, blood flow, oxygen consumption oxygenation variability, which reflects vasomotion activity, maximal force local fatigability) the hemorheological profile at rest between 16 healthy subjects (AA), 20 cell-hemoglobin C disease (SC) patients anemia (SS) patients.Muscle was reduced SS to SC AA...
The six-minute walk test is a well-established submaximal exercise reflecting the functional status and clinical severity of sickle cell patients. aim present cross-sectional study was to investigate biological determinants performance in children with anemia. Hematological hemorheological parameters, pulmonary function were determined 42 anemia at steady state. during normalized for age, sex height expressed as percentage predicted distance. We showed that high level anemia, low fetal...
The aim of the study was to determine factors associated with resting and exercise-induced hemoglobin oxygen desaturation. well-established six-minute walk test conducted in 107 sickle cell children (50 C disease 57 anemia) at steady state. Hemoglobin saturation measured before immediately after test. Blood samples were obtained on same day measure hematologic hemorheological parameters. Exercise-induced desaturation defined as a drop 3% or more end compared levels. No disease, but...
Little is known about the impact of blood rheology on occurrence retinopathy in sickle cell disease (SCD). Fifty-nine adult SCD patients steady-state condition participated to study: 32 with homozygous (sickle anemia; SCA) and 27 hemoglobin-C (SCC). The underwent retinal examination were categorized according classification Goldberg: 1) no (group 1), 2) non-proliferative or proliferative stage I-II 3) III-IV-V 3). Hematological hemorheological (whole viscosity, RBC deformability aggregation...
The aim of the present study was to test effects hydroxyurea (HU) therapy on clinical, hematological and hemorheological parameters in adult patients with sickle cell anemia (SCA). Hematological were measured 28 SCA before HU (i.e., baseline ) at 6, 12 24 months treatment. RBC deformability determined by ektacytometry 30 Pa. aggregation properties investigated light-backscatter method. Blood viscosity 225 s–1 a cone-plate viscometer. rates vaso-occlusive crises acute chest syndrome lower 1 2...
Patients with sickle cell anemia (SCA) have usually lower diastolic, systolic and mean blood pressure (BP) than the general population. However, BP values ≥120/70 mmHg considerably increase risk for acute chronic complications in SCA. The aim of this study was to identify biological factors associated relative hypertension adults We compared hematological, lipid hemolytic profiles, as well viscosity, between SCA patients normal (<120/70 mmHg, n = 54) those (BP≥120/70 43). Our results...
Vascular function has been found to be impaired in patients with sickle cell disease (SCD). The present study investigated the determinants of systemic vascular resistance two main SCD syndromes children: anemia (SCA) and cell-hemoglobin C (SCC). Nitric oxide metabolites (NOx), hematological, hemorheological, hemodynamical parameters were 61 children SCA 49 SCC. While mean arterial pressure was not different between SCC children, (SVR) greater children. Although SVR blood viscosity (ηb)...
Heat stress is one of the major limiting factors production efficiency in swine industry. The aims present study were 1) to observe if hemorheological and hematological parameters could be associated physiological acclimation during first days heat exposure 2) determine water restriction modulate effect thermal on physiological, parameters. Twelve Large White male pigs divided into an ad libitum a restricted group. All submitted week at 24°C (D-7 D-1). Then, D0, temperature was progressively...
A decreased global autonomic nervous system (ANS) activity and increased sympathetic activation in patients with sickle cell anemia (SCA) seem to worsen the clinical severity could play a role pathophysiology of disease, notably by triggering vaso-occlusive crises. Because exercise challenges ANS general population, we sought determine whether short (<15 min) progressive moderate session conducted until first ventilatory threshold had an effect on group SCA healthy individuals (CONT group)....