A5004601660- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Enzyme Catalysis and Immobilization
- Probiotics and Fermented Foods
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Diet, Metabolism, and Disease
- ATP Synthase and ATPases Research
- Polysaccharides and Plant Cell Walls
- Pancreatic function and diabetes
- Protein Structure and Dynamics
- Catalysis for Biomass Conversion
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
KTH Royal Institute of Technology
2021-2023
AlbaNova
2023
(1,3;1,4)-β-D-Glucans are widely distributed in the cell walls of grasses (family Poaceae) and closely related families, as well some other vascular plants. Additionally, they have been found organisms, including fungi, lichens, brown algae, charophycean green bacterium Sinorhizobium meliloti. Only three members Cellulose Synthase-Like (CSL) genes families CSLF, CSLH, CSLJ implicated (1,3;1,4)-β-D-glucan biosynthesis grasses. Little is known about enzymes responsible for synthesizing...
Clostridium thermocellum is a cellulolytic thermophile that considered for the consolidated bioprocessing of lignocellulose to ethanol. Improvements in ethanol yield are required industrial implementation, but incompletely understood causes amino acid secretion impede progress. In this study, was investigated via gene deletions ammonium-regulated, nicotinamide adenine dinucleotide phosphate (NADPH)-supplying and NADPH-consuming pathways as well physiological characterization...
The native ability of
Clostridium thermocellum is a promising candidate for consolidated bioprocessing of lignocellulosic biomass to ethanol. The low ethanol tolerance this microorganism one the remaining obstacles industrial implementation. Ethanol inhibition can be caused by end-product and/or chaotropic-induced stress resulting in increased membrane fluidization and disruption macromolecules. highly reversible glycolysis C. might especially sensitive inhibition. chaotropic effect known increase with...
The atypical glycolysis of Clostridium thermocellum is characterized by the use pyrophosphate (PPi) as a phosphoryl donor for phosphofructokinase (Pfk) and pyruvate phosphate dikinase (Ppdk) reactions. Previously, biosynthetic PPi was calculated to be stoichiometrically insufficient drive glycolysis. This study investigates role H+-pumping membrane-bound pyrophosphatase, glycogen cycling, predicted Ppdk-malate shunt cycle, acetate cycling in generating PPi. Knockout studies enzyme assays...
Abstract BACKGROUND Clostridium thermocellum is a promising candidate for consolidated bioprocessing of lignocellulosic biomass to ethanol. The low ethanol tolerance this microorganism one the remaining obstacles industrial implementation. Ethanol inhibition can be caused by end-product and/or chaotropic-induced stress resulting in increased membrane fluidization and disruption macromolecules. highly reversible glycolysis C. might especially sensitive inhibition. chaotropic effect known...
ABSTRACT Clostridium thermocellum is a cellulolytic thermophile considered for consolidated bioprocessing of lignocellulose to ethanol. Improvements in ethanol yield are required industrial implementation, but incompletely understood causes amino acid secretion impede progress. In this study, was investigated by gene deletions ammonium-regulated NADPH-supplying and -consuming pathways physiological characterization cellobiose- or ammonium-limited chemostats. First, the contribution malate...