A5049435336- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Insect Resistance and Genetics
- Insect and Pesticide Research
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Insect and Arachnid Ecology and Behavior
- Insect behavior and control techniques
- Immune Response and Inflammation
- Physiological and biochemical adaptations
- Mitochondrial Function and Pathology
- interferon and immune responses
- Biofuel production and bioconversion
- Photosynthetic Processes and Mechanisms
- Neurogenetic and Muscular Disorders Research
- Cancer-related molecular mechanisms research
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related Molecular Pathways
Fundación Instituto Leloir
2014-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2025
University of Buenos Aires
2014-2025
Fundación Ciencias Exactas y Naturales
2013-2025
Weizmann Institute of Science
1996-1999
Icahn School of Medicine at Mount Sinai
1993-1995
Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs constitutive further induced by stress. Both foci highly dynamic, with messenger ribonucleoproteins (mRNPs) proteins rapidly shuttling in out. Here, we show that impairment of retrograde transport knockdown mammalian dynein heavy chain 1 (DHC1) or bicaudal D1 (BicD1) inhibits SG formation PB growth without affecting protein-synthesis...
Stress granules are cytoplasmic mRNA-silencing foci that form transiently during the stress response. harbor abortive translation initiation complexes and in dynamic equilibrium with translating polysomes. Mammalian Staufen 1 (Stau1) is a ubiquitous double-stranded RNA-binding protein associated Here, we show Stau1 recruited to upon induction of endoplasmic reticulum or oxidative as well induced by blockers. We found lacking formed cells depleted this molecule, indicating not an essential...
Staufen is a conserved double-stranded RNA-binding protein required for mRNA localization in Drosophila oocytes and embryos. The mammalian homologues 1 2 have been implicated dendritic RNA targeting neurons. Here we show that rodent oligodendrocytes, these two proteins are present independent sets of granules located at the distal myelinating processes. A third kind lacks contains major myelin mRNAs. Myelin associate with microfilaments microtubules, their subcellular distribution affected...
Human immunodeficiency virus type 1 (HIV-1) Gag selects for and mediates genomic RNA (vRNA) encapsidation into progeny particles. The host protein, Staufen1 interacts directly with is found in ribonucleoprotein (RNP) complexes containing vRNA, which provides evidence that plays a role vRNA selection encapsidation. In this work, we show Staufen1, are the same RNP complex. These cellular viral factors also colocalize cells constitute novel RNPs (SHRNPs) whose assembly strictly dependent on...
Cytoplasmic events depending on RNA-binding proteins contribute to the fine-tuning of gene expression. Sterile α motif-containing constitute a novel family post-transcriptional regulators that recognize specific RNA sequence motif known as Smaug recognition element (SRE). The Drosophila member this family, dSmaug, triggers translational repression and deadenylation maternal mRNAs by independent mechanisms, yeast homologue Vts1 stimulates degradation SRE-containing messengers. Two homologous...
Hypoxia-inducible factors (HIFs) are a family of evolutionary conserved alpha-beta heterodimeric transcription that induce wide range genes in response to low oxygen tension. Molecular mechanisms mediate oxygen-dependent HIF regulation operate at the level alpha subunit, controlling protein stability, subcellular localization, and transcriptional coactivator recruitment. We have conducted an unbiased genome-wide RNA interference (RNAi) screen Drosophila cells aimed identification required...
Stress granules (SGs) are ephemeral cytoplasmic aggregates containing stalled translation preinitiation complexes involved in mRNA storage and triage during the cellular stress response. SG formation is triggered by phosphorylation of alpha subunit eIF2 (eIF2α), which provokes a dramatic blockage protein translation. Our results demonstrate that acute infection Vero cells with arenavirus Junín (JUNV), aetiological agent Argentine haemorrhagic fever, does not induce SGs. Moreover, JUNV...
Mammalian Smaug1/Samd4A is a translational repressor. Here we show that Smaug1 forms mRNA-silencing foci located at postsynapses of hippocampal neurons. These structures, which have named S-foci, are distinct from P-bodies, stress granules, or other neuronal RNA granules hitherto described, and the first described specific to binding was not required for aggregation, indicates S-foci formation consequence mRNA silencing. N-methyl-d-aspartic acid (NMDA) receptor stimulation provoked rapid...
Significance Emerging evidence indicates that posttranslational hydroxylation of intracellularly localized proteins is more prevalent than once thought. We identify Drosophila melanogaster sudestada1 (sud1) as a gene needed for normal growth in the fly and show sud1 encodes prolyl-hydroxylase catalyzes conserved residue small ribosomal subunit protein RPS23. Knockdown Sud1 results impairment reduced RPS23 hydroxylation, which associated with activation unfolded response, induction apoptosis,...
The adipose tissue plays a crucial role in metabolism and physiology, affecting animal lifespan susceptibility to disease. In this study, we present evidence that Dicer1 (Dcr-1), conserved type III endoribonuclease involved miRNA processing, the regulation of metabolism, stress resistance, longevity. Our results indicate expression Dcr-1 murine 3T3L1 adipocytes is responsive changes nutrient levels subject tight Drosophila fat body, analogous human hepatic tissues, under various...
Biomolecular condensates (BMCs) emerge as important players in RNA regulation. The binding protein Smaug forms cytosolic BMCs mammals, insects and yeasts affects mitochondrial function and/or responses to nutrient deprivation. Here we found that the non-canonical activation of SMO-AMPK pathway known affect energy metabolism triggers immediate disassembly formed by a number human rodent orthologs, whereas processing bodies remained rather unaltered. A non-phosphorylatable SMO mutant abrogated...
Repression of mRNA translation is linked to the formation specific cytosolic foci such as Stress Granules (SGs) and Processing Bodies (PBs), which store or degrade mRNAs. In neurons, synaptic activity regulates at postsynapse this important for plasticity. NMDA receptor stimulation downregulates we speculate that unknown mRNA-silencing foci. Here show 5′-3′ exoribonuclease XRN1 forms discrete clusters associated are different from PBs SGs, named them Synaptic XRN1-bodies (SX-bodies). Using...
The dynamic formation of stress granules (SGs), processing bodies (PBs), and related RNA organelles regulates diverse cellular processes, including the coordination functionally connected messengers, translational regulation at synapse, control viruses retrotransposons. Recent studies have shown that pyruvate kinase other enzymes localize in SGs PBs, where they become protected from insults. These observations may implications for enzyme metabolic exerted by RNA-based organelles. these is...
Regulatory sequences used in plasmids for naked DNA vaccination can modulate cytokine production vivo. We demonstrate here that injection of plasmid suppress the prototypic T cell-mediated autoimmune disease, experimental encephalomyelitis, by inducing IFN-γ.
The transcriptome at the synapse consists of thousands messengers encoding a plethora cellular functions, including an important number receptors and ion channels associated proteins. concerted translational regulation all these molecules contributes to dynamic control synaptic strength. Cumulative evidence supports role for dendritic RNA granules mRNA-silencing foci in regulation, several RBPs ‒FMRP, FUS/TLS, TDP-43, Staufen, Smaug, Pumilio, CPEB, HuD, ZBP1 DDX6 among others‒ form that...
The regulated synthesis of specific proteins at the synapse is important for neuron plasticity, and several localized mRNAs are translated upon stimulus. Repression mRNA translation linked to formation mRNA-silencing foci, including Processing Bodies (PBs) Stress Granules (SGs), which macromolecular aggregates that harbor silenced messengers associated proteins. In a recent work, we identified kind foci unique neurons, termed S-foci, contain post-transcriptional regulator Smaug1/SAMD4. Upon...
Many of mRNAs synthesized during pollen development are translated after germination, and we hypothesize that they stored in cytoplasmic granules. We analyzed the cellular localization SKS14 AT59 Arabidopsis mRNAs, which orthologues tobacco NTP303 tomato LAT59 respectively, by artificially labeling transcripts with a MS2-GFP chimera. A MATLAB-automated image analysis helped to identify presence mRNA granules mature grains. These partially colocalized VCS DCP1, two processing body (PB)...
Smaug is a conserved translational regulator that binds numerous mRNAs, including nuclear transcripts encode mitochondrial enzymes. orthologs form cytosolic membrane-less organelles (MLOs) in several organisms and cell types. We have performed single-molecule fluorescence situ hybridization (FISH) assays revealed SDHB UQCRC1 mRNAs associate with Smaug1 bodies U2OS cells. Loss of function Smaug2 (also known as SAMD4A SAMD4B, respectively) affected both respiration morphology the network....
Abstract Changes in morphology during early metamorphosis of the medfly, Ceratitis capitata (Wied.) ( Tephritidae ) were correlated with biochemical differentiation events. Protein profiles studied both 3rd instar larval cuticle further transformed into puparium and newly synthesized pupal cuticle. Beta‐alanine incorporation (0–20 h) correlates concomitant pigmentation (completed by 16 sclerotization phenomena. This ‘tannification program seems to be followed deposition a layer substances,...
The multiple display of protein domains on polymeric scaffolds is an emerging technology for many applications. BLS a highly immunogenic that has oligomeric structure formed by 17.2 kDa subunit arranged as dimer pentamers. Here we describe the production well structural, functional, and immunological properties 9 double-stranded RNA-binding domain (RBD3) fused to BLS. We demonstrate RBD3 modules are stably independently folded in chimera form decameric 255 native oligomers. increases dsRNA...