A5008150245- Immune Response and Inflammation
- interferon and immune responses
- Influenza Virus Research Studies
- CRISPR and Genetic Engineering
The University of Queensland
2022-2025
Innate immunity relies on Toll-like receptors (TLRs) to detect pathogen-associated molecular patterns. The TIR (Toll/interleukin-1 receptor) domain-containing TLR adaptors TRIF (TIR adaptor-inducing interferon-β) and TRAM (TRIF-related adaptor molecule) are essential for MyD88-independent signaling. However, the structural basis of domain-based signaling remains unclear. Here, we present cryo-EM structures filaments formed by domains at resolutions 3.3 Å 5.6 Å, respectively. Both reveal...
Conventional assays to probe signaling protein interactions and function involve measurement of luciferase reporter expression within the bulk cell population, with lack control over target-protein level. To address this issue, we have developed a rapid robust flow cytometric assay for analysis function. A fluorescent tagging target enables simultaneous assessment individual cells. We applied our technique variants lipopolysaccharide receptor Toll-like 4 (TLR4) its adapter MyD88, using...