A5007838107- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Vector-Borne Animal Diseases
- Leptospirosis research and findings
- Animal health and immunology
- Vector-borne infectious diseases
- Parasites and Host Interactions
- Animal Nutrition and Physiology
- Plant Surface Properties and Treatments
- Helminth infection and control
- Agricultural and Food Sciences
- Insect Pest Control Strategies
- Mast cells and histamine
- Urticaria and Related Conditions
- Male Reproductive Health Studies
- Blood Coagulation and Thrombosis Mechanisms
- Bird parasitology and diseases
- Aquaculture disease management and microbiota
- Entomological Studies and Ecology
- Livestock and Poultry Management
- Parasite Biology and Host Interactions
- Viral gastroenteritis research and epidemiology
- Animal Genetics and Reproduction
- Dermatological diseases and infestations
- Insect behavior and control techniques
Universidade de São Paulo
2017-2024
Governo do Estado de São Paulo
2020
Fundación Pública Andaluza para la Investigación de Málaga en Biomedicina y Salud
2020
Universidade Estadual Paulista (Unesp)
2013-2020
Leishmaniases are important neglected tropical diseases caused by protozoans of the genus Leishmania spp. The female sandflies bite (Diptera: Psychodidae: Phlebotominae) is main transmission form in epidemiological chains these diseases. Thus, studies on Phlebotominae subfamily fundamental to improve comprehension leishmaniases epidemiology, revealing relationships dipterans with parasite and reservoirs. An entomological survey Phlebotomine was carried out investigate fauna, DNA detection...
Atualmente a pesca subaquática tem se destacado como um dos principais meios para captura de peixes partir da utilização técnicas mergulho com petrechos que envolve o uso arpões, sendo classificada uma modalidade não esportiva. O objetivo desta pesquisa foi analisar prevalência bactérias patogênicas na carne abatidos arpões prática subaquática. Diferentes espécies foram capturadas arpão nos rios Tietê e Paraná, região noroeste do estado São Paulo. Os ficaram presos em fieiras dentro água...
This study aimed to investigate the occurrence of Lutzomyia longipalpis and also canine visceral leishmaniasis (CVL) in a rural area Ilha Solteira, state São Paulo. Blood samples were collected from 32 dogs different properties (small farms) analyzed by ELISA indirect immunofluorescence antibody test (IFAT) order diagnose CVL. From these serological tests, 31.25% positive for CVL distributed 66.7% (8/12) properties, which L. longipalpis. CDC (Center Disease Control Prevention) light traps...
The aim of this study was to compare molecular tests used diagnose Leishmania spp. in dogs with different stages infection. Blood and conjunctival swab (CS) samples from classified four clinical were subjected PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) as without signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) II (moderate disease) 3.2% (7/215) III (severe disease). results showed that blood samples, 13A/13B detected a...
We sought to determine risk factors (RFs) associated with the presence of antibodies against Leishmania in dogs from a rural area Ilha Solteira, SP, Brazil.Serum samples were collected 250 and tested using indirect enzyme-linked immunosorbent assay (ELISA) immunofluorescence antibody tests (IFATs). Data concerning dogs, their environment, owners' knowledge leishmaniasis questionnaire. To RFs for contact parasite, univariate statistical analysis based on chi-squared Fisher's exact tests,...
Leishmaniasis is a neglected tropical disease that continues to pose serious public health problem. Albeit dogs have long been held as the major reservoirs of Leishmania infantum, involvement domestic cats in zoonotic cycle visceral leishmaniasis has gained prominence. Here, 240 were evaluated by clinical signs and haematological/biochemical changes compatible with diagnosed serological, molecular, parasitological techniques. Thus, four naturally infected L. infantum submitted xenodiagnosis....
The aim of this work was a correlation study and histopathological description alterations associated with the presence Leishmania infantumamastigote in intestinal wall dogs infected canine visceral leishmaniasis (CVL). Three groups were used: G1 (n = 8), comprising naturally CVL amastigotes L. infantum small large intestines; G2 9), CVL, without amastigotes; G3 3), uninfected dogs. Histochemistry immunohistochemistry methods used for histopathology identification. 47.1% (8/17) from group...
An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood conjunctival swab (CS) samples were collected from 94 cats two animal protection shelters. These subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) enzyme-linked immunosorbent assay (ELISA) molecular by polymerase chain reaction (PCR). In addition, a same The analyses revealed positivity 31.91%...
One hundred and sixty-six cats from two animal shelters were subjected to enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), conventional polymerase chain reaction (cPCR), quantitative PCR (qPCR) parasitological tests (PA) for the diagnosis of Leishmania spp. Among them, 15% (25/166), 53.6% (89/166), 3.6% (06/166) 1.8% (03/166) positive by ELISA, IFAT, both PCRs PA, respectively. The sequencing ITS-1 amplicons revealed a 100% match with infantum....
This study was about a semi-quantitative analysis of T lymphocytes (CD4+ and CD8+, FoxP3+ regulatory cells), macrophages in the gut wall dogs naturally infected with Leishmania infantum. Thirteen were divided into three groups: group 1 (G1, n=5), canine visceral leishmaniasis (CVL) L. infantum amastigotes intestine; 2 (G2, CVL but without intestinal amastigotes; 3 (G3, n=3), uninfected (control group). There no significant difference (p ≥ 0.05) on CD4+ Treg cell numbers among groups, whereas...
Visceral leishmaniasis (VL) is a disease caused by the protozoa Leishmania infantum and can cause an inflammatory reaction in gastrointestinal tract, however role of granulocytic cells (neutrophils, eosinophils, mast cells) intestine dogs infected not fully understood. We performed quantitative analysis these intestinal wall with canine visceral (CVL). Twenty were assigned to one three groups: group 1 (G1, n=8), CVL L. amastigotes intestine; 2 (G2, n=9), but without amastigotes; 3 (G3, n=3),...
O experimento foi realizado com o objetivo de avaliar a administração do óleo Neem na redução dos valores ovos por gramas fezes em ovinos naturalmente infectados nematóides e protozoários gastrintestinais, verificar dinâmica populacional endoparasitas através da coprocultura os padrões hematológicos, bioquímicos, ganho peso escore corporal, condições manejo pastagens. Foram utilizados 32 mestiços Santa Inês divididos dois grupos (C - Controle T tratados Neem), uniformes vivo acordo...
ABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. in the reproductive organs of male female dogs. histochemistry, immunohistochemistry, PCR, examined tissue samples from 8 dogs 16 diagnosed with Despite absence macroscopic or microscopic lesions these organs, observed amastigotes testis uterus. PCR sequencing tissues revealed sequences that matched 100% DNA available at GenBank. The presence testicular uterine suggested...
Piranhas são peixes de pequeno a médio porte endêmicos em diferentes regiões do Brasil. Estes estão amplamente distribuídos nos rios e lagos da região noroeste estado São Paulo numerosos os relatos acidentes envolvendo espécies piranhas com pescadores banhistas. gênero Serrassalmus foram capturadas utilizando isca viva lambari (Astianaxspp.). Paralelamente à lesão causada pela mordedura piranha foi feita uma incisão lâmina bisturi aço carbono nº22 primeiro uso para se comparar por meio...
A grande diversidade das espécies de Aves Silvestres presentes na região Ilha Solteira exige o conhecimento destas. Um desequilíbrio negativo desse ambiente como a construção uma hidrelétrica pode fazer com que esses animais sofram problemas, desde ectoparasitas até endoparasitas e doenças graves. Portanto, objetivo do presente estudo foi identificar ocorrência infestações endoparasitarias em aves silvestres mantidas...
O objetivo foi estudar a presença de neutrófilos e eosinófilos na região intestinal cães naturalmente infectados com Leishmania infantum em ausência do parasita. Foram analisados 11 positivos para Leishmaniose Visceral Canina (LVC) diagnosticados eutanasiados pelo Centro Controle Zoonoses (CCZ) da cidade Ilha Solteira, SP. Dois grupos foram estabelecidos: LVC amastigotas no intestino (P, n=5) LVC, mas sem parasita (N, n=6). Secções...
Na área rural denominada por “Cinturão Verde” do município de Ilha Solteira, localizado na região Noroeste estado São Paulo (S 20º25'36,47"; O 51º 20' 26,47"), foi realizada a captura flebotomíneos ( Lutzomyia longipalpis ) utilizando armadilhas luminosas tipo CDC Center for Disease Control and Prevention ). As capturas foram realizadas das 17 às 07 horas, três vezes consecutivas/mês. Foram colocadas 12 no...