A5072342252- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Vector-Borne Animal Diseases
- Toxoplasma gondii Research Studies
- T-cell and Retrovirus Studies
- Leptospirosis research and findings
- Parasites and Host Interactions
- Public Health in Brazil
- Male Reproductive Health Studies
- Animal Disease Management and Epidemiology
- Livestock and Poultry Management
- Comparative Animal Anatomy Studies
- Youth, Drugs, and Violence
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Zoonotic diseases and public health
- Parasite Biology and Host Interactions
- Blood Coagulation and Thrombosis Mechanisms
- Dermatological diseases and infestations
- Animal Genetics and Reproduction
- Fossil Insects in Amber
- Rabies epidemiology and control
Universidade Brasil
2019-2025
Universidade de São Paulo
2017-2025
Leishmaniases are important neglected tropical diseases caused by protozoans of the genus Leishmania spp. The female sandflies bite (Diptera: Psychodidae: Phlebotominae) is main transmission form in epidemiological chains these diseases. Thus, studies on Phlebotominae subfamily fundamental to improve comprehension leishmaniases epidemiology, revealing relationships dipterans with parasite and reservoirs. An entomological survey Phlebotomine was carried out investigate fauna, DNA detection...
Abstract In this study, an evaluation was made of three treatments against feline leishmaniosis (FeL) and their impacts on the transmission Leishmania infantum to its vector, Lutzomyia longipalpis. A cat with clinical signs FeL examined L. diagnosed. Subsequently, subjected xenodiagnosis detected in vectors. The then treated different drugs improvement parasite transmissibility vector were evaluated. Promastigotes observed 21/52 female sandflies (40.38%) a prior treatments. Clinical...
The aim of this study was to compare molecular tests used diagnose Leishmania spp. in dogs with different stages infection. Blood and conjunctival swab (CS) samples from classified four clinical were subjected PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) as without signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) II (moderate disease) 3.2% (7/215) III (severe disease). results showed that blood samples, 13A/13B detected a...
Leishmaniasis is a neglected tropical disease that continues to pose serious public health problem. Albeit dogs have long been held as the major reservoirs of Leishmania infantum, involvement domestic cats in zoonotic cycle visceral leishmaniasis has gained prominence. Here, 240 were evaluated by clinical signs and haematological/biochemical changes compatible with diagnosed serological, molecular, parasitological techniques. Thus, four naturally infected L. infantum submitted xenodiagnosis....
An epidemiological Leishmania spp. and entomological Phlebotomine sandflies survey was performed in cat shelters at leishmaniasis endemic area of Brazil. Blood conjunctival swab (CS) samples were collected from 94 cats two animal protection shelters. These subjected to serological tests using the indirect immunofluorescence antibody test (IFAT) enzyme-linked immunosorbent assay (ELISA) molecular by polymerase chain reaction (PCR). In addition, a same The analyses revealed positivity 31.91%...
One hundred and sixty-six cats from two animal shelters were subjected to enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), conventional polymerase chain reaction (cPCR), quantitative PCR (qPCR) parasitological tests (PA) for the diagnosis of Leishmania spp. Among them, 15% (25/166), 53.6% (89/166), 3.6% (06/166) 1.8% (03/166) positive by ELISA, IFAT, both PCRs PA, respectively. The sequencing ITS-1 amplicons revealed a 100% match with infantum....
Visceral leishmaniasis (VL) is a neglected disease considered serious public health problem, especially in endemic countries. Several studies have discovered monoxenous trypanosomatids (Leptomonas and Crithidia) patients with VL. In different situations of leishmaniasis, investigations examined cases co-infection between Leishmania spp. Crithidia These coinfections been observed wide range vertebrate hosts, indicating that they are not rare. Diagnostic techniques require improvements more...
Abstract Leishmania spp. are important agents of human and animal leishmaniases that have an impact on public health. In this study, we aimed to detect the circulation in cattle from a visceral leishmaniasis non-endemic area state São Paulo, Brazil. DNA was extracted blood samples 100 heifers municipality Pirassununga amplified using primers specific for first internal transcriber spacer (ITS1), assess presence trypanosomatids. The assays revealed one sample presented bands between 300 350...
Visceral leishmaniasis (VL) is a neglected tropical disease caused by the Leishmania infantum parasite. The protozoan able to infect several domestic and wild mammals. Since first report on spp. infection in horses South America, equids has been highlighted Brazil. A molecular epidemiological survey was carried out verify occurrence of DNA donkeys, leishmaniases endemic areas Sao Paulo State, To this end, blood samples were obtained from 107 36 donkeys subjected extraction followed PCR...
INTRODUCTION Visceral leishmaniasis (VL) transmission has been associated with two different populations of the Lutzomyia longipalpis complex in São Paulo state. METHODS In a recent focus VL, we captured and dissected sand flies investigated Leishmania infantum infection by parasitological, PCR, sequencing analysis. RESULTS Flagellates were observed 2 47 (4.2%) cembrene-1 Lu. females. The sequences obtained matched those Le. infantum. CONCLUSIONS We found that females may occur at high rate...
ABSTRACT: Leishmania infantum causes canine leishmaniasis. Using parasitological and molecular analyses, we identified L. in the reproductive organs of male female dogs. histochemistry, immunohistochemistry, PCR, examined tissue samples from 8 dogs 16 diagnosed with Despite absence macroscopic or microscopic lesions these organs, observed amastigotes testis uterus. PCR sequencing tissues revealed sequences that matched 100% DNA available at GenBank. The presence testicular uterine suggested...
Many owners believe to act in their pets' best interest by allowing them go outside, with or without supervision. However, outdoor exposure greatly increases the risk of disease and accidents. In this study, we evaluated association between infectious parasitic diseases habits pet dogs cats. Epidemiological data were obtained from records cats treated at Teaching Clinic Hospital Unit Veterinary Medicine city Pirassununga, state São Paulo, Brazil. Pets any form infection included case study...
O presente trabalho teve como objetivo identificar anticorpos anti-Neospora caninum em fêmeas bovinas da raça Nelore pelos métodos sorológicos de Imunoabsorbância Ligada a Enzima (ELISA) e Imunofluorescência Indireta (RIFI) buscar associação entre soropositividade o histórico reprodutivo até 60 dias gestação. Adicionalmente, nas amostras positivas foram realizados testes ELISA-Avidez ELISA para anti-Toxoplama gondii. Para isto, foi constituído um banco 375 soros, categorizados três grupos,...